Invitrogen

c-Myc Monoclonal Antibody (9E10), HRP

FIGURE: 1 / 3

c-Myc Antibody (MA1-980-HRP) in WB

Western blot was performed using Anti-Myc Tag Monoclonal Antibody (Product # MA1-980-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293 lysates: untransfected, 40 µg (Lane 1), empty vector control, 60 µg (Lane 2), Myc-p65-V5, 60 µg (Lane 3), Myc-p65-V5, 40 µg (Lane 4), Myc-p65-V5, 20 µg (Lane 5), Myc-p65-V5, 10 µg (Lane 6), p65-HA, 60 µg (Lane 7) and 25 ng of Positope (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred on... View More

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Product Details

MA1-980-HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9E10

Immunogen

Synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.

View immunogen

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

proprietary buffer with proprietary stabilizer

Contains

0.07% Kathon

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537628

Product Specific Information

MA1-980-HRP detects c-myc protein and c-myc tagged proteins.

MA1-980-HRP has been successfully used in Western blot procedures.

The MA1-980-HRP immunogen corresponds to the synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.

Target Information

The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Excess nitrogen responsive HvMADS27 transcription factor controls barley root architecture by regulating abscisic acid level.

Frontiers in plant science

Smoczynska A,Pacak A,Grabowska A,Bielewicz D,Zadworny M,Singh K,Dolata J,Bajczyk M,Nuc P,Kesy J,Wozniak M,Ratajczak I,Harwood W,Karlowski WM,Jarmolowski A,Szweykowska-Kulinska Z

Published figure using c-Myc monoclonal antibody (Product # MA1-980-HRP) in Western Blot

Mon Sep 18 00:00:00 EDT 2023

A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation.

Immunity

Liu Z,Nailwal H,Rector J,Rahman MM,Sam R,McFadden G,Chan FK

MA1-980-HRP was used in Flow Cytometry to identify a viral inhibitor that binds to the host SKP1-Cullin1-F-box (SCF) machinery and the essential necroptosis kinase receptor interacting protein kinase 3 (RIPK3).

Tue Feb 09 00:00:00 EST 2021

Proteolytic degradation of regulator of G protein signaling 2 facilitates temporal regulation of G_q/11 signaling and vascular contraction.

The Journal of biological chemistry

Kanai SM,Edwards AJ,Rurik JG,Osei-Owusu P,Blumer KJ

MA1-980-HRP was used in Western Blotting to identify degradation signals in regulator of G protein signalling 2, and study the dynamic regulation of Gq/11-evoked Ca2+ signalling and vascular contraction.

Fri Nov 24 00:00:00 EST 2017

HCMV-encoded glycoprotein M (UL100) interacts with Rab11 effector protein FIP4.

Traffic (Copenhagen, Denmark)

Krzyzaniak MA,Mach M,Britt WJ

MA1-980-HRP was used in Immunocytochemistry to argue for a contribution of the ERC during cytoplasmic envelopment of HCMV and showed a novel FIP4 function independent of Arf5 or Arf6 activity.

Thu Oct 01 00:00:00 EDT 2009

HCMV-encoded glycoprotein M (UL100) interacts with Rab11 effector protein FIP4.

Traffic (Copenhagen, Denmark)

Krzyzaniak MA,Mach M,Britt WJ

MA1-980-HRP was used in Immunocytochemistry to argue for a contribution of the ERC during cytoplasmic envelopment of HCMV and showed a novel FIP4 function independent of Arf5 or Arf6 activity.

Thu Oct 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog

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Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC

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UniProt ID: (Human) P01106

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Entrez Gene ID: (Human) 4609

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein complex binding protein dimerization activity repressing transcription factor binding E-box binding basic helix-loop-helix transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter MAPK cascade branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation energy reserve metabolic process chromatin remodeling transcription from RNA polymerase II promoter cellular iron ion homeostasis cellular response to DNA damage stimulus cell cycle arrest Notch signaling pathway positive regulation of cell proliferation response to gamma radiation regulation of gene expression oxygen transport regulation of telomere maintenance negative regulation of stress-activated MAPK cascade cellular response to UV cellular response to drug response to drug negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process fibroblast apoptotic process negative regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation negative regulation of fibroblast proliferation positive regulation of epithelial cell proliferation chromosome organization negative regulation of cell division canonical Wnt signaling pathway response to growth factor positive regulation of metanephric cap mesenchymal cell proliferation beta-catenin-TCF complex assembly positive regulation of DNA biosynthetic process positive regulation of response to DNA damage stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Myc Monoclonal Antibody (9E10), HRP

c-Myc Antibody (MA1-980-HRP) in WB

Product Details

MA1-980-HRP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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