GenScript

beta-tubulin Monoclonal Antibody (2G7D4)

beta-tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

beta-tubulin Antibody (A01717-40) in IHC (P)

Immunohistochemistry analysis of human colon tissue slide (Paraffin embedded) using beta-tubulin Antibody (2G7D4), mAb, Mouse (GenScript, Cat. No. A01717, 15 µg/mL).

beta-tubulin Antibody in Western Blot (WB)

beta-tubulin Antibody in Flow Cytometry (Flow)

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Product Details

A01717-40

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

Flow Cytometry (Flow)

1 µg 1 x 10^6 cells

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Chicken, Fish, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2G7D4

Immunogen

purified human recombinant full-length beta-tubulin protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (a) and beta (ß) chains. ß-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots.GenScript ß-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length ß-tubulin protein.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway.

Journal of Alzheimer's disease : JAD

Fisher CL,Resnick RJ,De S,Acevedo LA,Lu KP,Schroeder FC,Nicholson LK

The cis/trans isomerization of X-Pro peptide bonds in proteins in some instances acts as a molecular switch in biological pathways. Our prior work suggests that the cis isomer of the phospho-Thr668-Pro669 motif located in the cytoplasmic domain of the amyloid-β protein precursor (AβPP) is correlated with an increase in amyloidogenic processing of AβPP and production of amyloid-beta (Aβ) the neurotoxic peptide fragment in Alzheimer's disease (AD). We designed a 100% cis-locked

Thu Feb 15 00:00:00 EST 2018

Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain.

The FEBS journal

Huang LH,Nishi K,Li S,Ho T,Dong R,Chang CC,Chang TY

Acyl-Coenzyme A:cholesterol Acyltransferases (ACATs), members of the membrane bound O-acyltransferase (MBOAT) family, catalyze the conversion of cholesterol to cholesteryl esters. Mammals contain two isoenzymes ACAT1 and ACAT2. Both enzymes are drug targets for treating human diseases. ACAT1 is present ubiquitously in various cell types. It contains 9 transmembrane domains (TMDs) with the active site H460 locating within TMD #7, and another active site N421 locating within the 4th large cytoplas

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: beta 5-tubulin; beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-4-tubulin; beta-5 tubulin; beta-tubulin; Beta-tubulin class-I; Beta-tubulin class-II; beta-tubulin class-III; Beta-tubulin class-V; Beta-tubulin class-VI; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; c(beta)7 tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; TUBB; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 4'; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-7 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 5; unnamed protein product

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Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8

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Entrez Gene ID: (Rabbit) 100349792, (Goat) 102168953, (Chicken) 396254, (Human) 203068, (Pig) 733686, (Rabbit) 100358689, (Bovine) 615087, (Chicken) 396427, (Pig) 100134983, (Bovine) 541271, (Goat) 102188330, (Human) 81027, (Rabbit) 100347858, (Chicken) 768337, (Goat) 108633269, (Human) 7280, (Human) 347733, (Chicken) 420883, (Bovine) 281555, (Pig) 100153507, (Bovine) 768070, (Human) 10381, (Chicken) 431043, (Bovine) 540236, (Human) 10382, (Goat) 102178062, (Pig) 100736584, (Rabbit) 100348290, (Pig) 100623583, (Rabbit) 100350252, (Goat) 102178409, (Human) 84617, (Chicken) 421037, (Bovine) 534206, (Human) 347688, (Mouse) 545486, (Rat) 679312, (Rat) 498736, (Mouse) 22151, (Mouse) 73710, (Rat) 291081, (Mouse) 22152, (Rat) 246118, (Rat) 29213, (Mouse) 22153, (Mouse) 22154, (Rat) 29214, (Mouse) 67951, (Rat) 307351

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Function(s)

GTPase activity structural constituent of cytoskeleton GTP binding ubiquitin protein ligase binding MHC class I protein binding structural molecule activity protein binding protein domain specific binding protein complex binding GTPase activating protein binding calcium ion binding protein heterodimerization activity netrin receptor binding peptide binding molecular_function nucleotide binding tubulin

Process(es)

microtubule cytoskeleton organization mitotic cell cycle microtubule-based process regulation of synapse organization spindle assembly G2/M transition of mitotic cell cycle movement of cell or subcellular component cellular process cytoskeleton-dependent intracellular transport natural killer cell mediated cytotoxicity cell division cytoskeleton organization neuron migration negative regulation of microtubule polymerization positive regulation of axon guidance axon guidance netrin-activated signaling pathway dorsal root ganglion development mitotic nuclear division neuron differentiation biological_process protein polymerization metabolic process cell projection organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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beta-tubulin Monoclonal Antibody (2G7D4)

beta-tubulin Antibody (A01717-40) in IHC (P)

Product Details

A01717-40

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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