Product References
Berberine Reduces Lipid Accumulation in Obesity via Mediating Transcriptional Function of PPARδ.
International journal of molecular sciences
Shou JW,Shaw PC
AM4302 was used in Western Blot to elucidate the role of PPARs in BBR treatment on obesity and the underlying molecular mechanisms.
Tue Jul 18 00:00:00 EDT 2023
A non-coding variant in the Kozak sequence of RARS2 strongly decreases protein levels and causes pontocerebellar hypoplasia.
BMC medical genomics
Nicolle R,Altin N,Siquier-Pernet K,Salignac S,Blanc P,Munnich A,Bole-Feysot C,Malan V,Caron B,Nitschké P,Desguerre I,Boddaert N,Rio M,Rausell A,Cantagrel V
AM4302 was used in Western Blotting to show here that this variant, additionally to its effect on the transcription, also disrupts the consensus Kozak sequence, and has a major impact on RARS2 protein translation.
Wed Jun 21 00:00:00 EDT 2023
Deletion of the mRNA stability factor ELAVL1 (HuR) in pancreatic cancer cells disrupts the tumor microenvironment integrity.
NAR cancer
McCarthy GA,Di Niro R,Finan JM,Jain A,Guo Y,Wyatt CR,Guimaraes AR,Waugh TA,Keith D,Morgan TK,Sears RC,Brody JR
AM4302 was used in Western Blotting to identify a novel role of tumor-intrinsic human antigen R in its ability to modify the surrounding tumor microenvironment and regulate platelet-derived growth factor AA (PDGFAA).
Thu Jun 01 00:00:00 EDT 2023
Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus.
iScience
Chen DY,Turcinovic J,Feng S,Kenney DJ,Chin CV,Choudhary MC,Conway HL,Semaan M,Close BJ,Tavares AH,Seitz S,Khan N,Kapell S,Crossland NA,Li JZ,Douam F,Baker SC,Connor JH,Saeed M
AM4302 was used in Western Blotting to establish a cell culture system using human cell lines overexpressing SARS-CoV-2 entry factors, particularly Caco-2/AT and HuH-6/AT, which demonstrated exceptional susceptibility, providing a robust platform for generating authentic SARS-CoV-2 stocks and studying the virus and its emerging variants.
Fri May 19 00:00:00 EDT 2023
Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus.
iScience
Chen DY,Turcinovic J,Feng S,Kenney DJ,Chin CV,Choudhary MC,Conway HL,Semaan M,Close BJ,Tavares AH,Seitz S,Khan N,Kapell S,Crossland NA,Li JZ,Douam F,Baker SC,Connor JH,Saeed M
AM4302 was used in Western Blotting to establish a cell culture system using human cell lines overexpressing SARS-CoV-2 entry factors, particularly Caco-2/AT and HuH-6/AT, which demonstrated exceptional susceptibility, providing a robust platform for generating authentic SARS-CoV-2 stocks and studying the virus and its emerging variants.
Fri May 19 00:00:00 EDT 2023
The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.
Nature cell biology
Krastev DB,Li S,Sun Y,Wicks AJ,Hoslett G,Weekes D,Badder LM,Knight EG,Marlow R,Pardo MC,Yu L,Talele TT,Bartek J,Choudhary JS,Pommier Y,Pettitt SJ,Tutt ANJ,Ramadan K,Lord CJ
AM4302 was used in Western Blot to identify an interaction between trapped PARP1 and the ubiquitin-regulated p97 ATPase/segregase.
Sat Jan 01 00:00:00 EST 2022
Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer.
American journal of human genetics
Nakamura K,Reid BM,Chen A,Chen Z,Goode EL,Permuth JB,Teer JK,Tyrer J,Yu X,Kanetsky PA,Pharoah PD,Gayther SA,Sellers TA,Lawrenson K,Karreth FA
AM4302 was used in Western Blotting to integrate in silico variant-to-function analysis with functional studies to characterize the oncogenic potential of candidate genes in the 1p34.3 locus.
Thu Jan 06 00:00:00 EST 2022
Recurrent evolution of an inhibitor of ESCRT-dependent virus budding and LINE-1 retrotransposition in primates.
Current biology : CB
Rheinemann L,Downhour DM,Davenport KA,McKeown AN,Sundquist WI,Elde NC
AM4302 was used in Western Blot to reveal the evolutionary process of detoxification for retroCHMP3 in New World monkeys using a combination of ancestral reconstructions, cytotoxicity, and virus release assays.
Mon Apr 11 00:00:00 EDT 2022
Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.
Oncology letters
Mao X,Wang J,Luo F
AM4302 was used in Western Blot to investigate the interaction between alpha-fetoprotein and metastasis-associated colon cancer 1.
Tue Mar 01 00:00:00 EST 2022
Mild membrane depolarization in neurons induces immediate early gene transcription and acutely subdues responses to a successive stimulus.
The Journal of biological chemistry
Rienecker KDA,Poston RG,Segales JS,Finholm IW,Sono MH,Munteanu SJ,Ghaninejad-Esfahani M,Rejepova A,Tejeda-Garibay S,Wickman K,Marron Fernandez de Velasco E,Thayer SA,Saha RN
AM4302 was used in Western Blotting to suggest that a recent history of graded potential changes acutely depress neuronal intrinsic properties and subsequent responses.
Thu Sep 01 00:00:00 EDT 2022
Caffeine Inhibits NLRP3 Inflammasome Activation by Downregulating TLR4/MAPK/NF-κB Signaling Pathway in an Experimental NASH Model.
International journal of molecular sciences
Vargas-Pozada EE,Ramos-Tovar E,Rodriguez-Callejas JD,Cardoso-Lezama I,Galindo-Gómez S,Talamás-Lara D,Vásquez-Garzón VR,Arellanes-Robledo J,Tsutsumi V,Villa-Treviño S,Muriel P
AM4302 was used in Western Blot to evaluate the effect of caffeine on the NLRP3 inflammasome signaling pathway in a rat model of NASH.
Thu Sep 01 00:00:00 EDT 2022
Prognostic value of integrin αV expression and localization pattern in invasive breast carcinomas.
Neoplasia (New York, N.Y.)
Cerqueira OLD,Botelho MCS,Fiore APZP,Osório CABT,Tomasin R,Morais MCC,López RVM,Cardoso EC,Vilella-Arias SA,Reis EM,Bruni-Cardoso A
AM4302 was used in Western Blotting to indicate that both the expression levels of integrin αV and its tissue localization in primary tumors have prognostic value, and thus, could be used to help predict patients at higher risk of developing metastasis.
Mon Aug 01 00:00:00 EDT 2022
lncRNA HAGLR modulates myocardial ischemia-reperfusion injury in mice through regulating miR-133a-3p/MAPK1 axis.
Open medicine (Warsaw, Poland)
Wang Z,Luo W,Zhong P,Feng Y,Wang H
AM4302 was used in Western Blot to show that knockdown of HAGLR could suppress inflammatory response and cell apoptosis induced by I/R and, thus, alleviate myocardial I/R injury.
Tue Aug 09 00:00:00 EDT 2022
β1 integrin monoclonal antibody treatment ameliorates cerebral cavernous malformations.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
McCurdy S,Lin J,Shenkar R,Moore T,Lightle R,Faurobert E,Lopez-Ramirez MA,Awad I,Ginsberg MH
AM4302 was used in Western Blotting to conclude that targeting β1 integrin with an activated-specific antibody reduces acute murine CCM lesion development, which was found to be associated with suppression of endothelial ROCK activity.
Thu Dec 01 00:00:00 EST 2022
Nuclear speckle integrity and function require TAO2 kinase.
Proceedings of the National Academy of Sciences of the United States of America
Gao S,Esparza M,Dehghan I,Aksenova V,Zhang K,Batten K,Ferretti MB,Begg BE,Cagatay T,Shay JW,García-Sastre A,Goldsmith EJ,Chen ZJ,Dasso M,Lynch KW,Cobb MH,Fontoura BMA
AM4302 was used in Western Blotting to find that a nuclear pool of TAO2 is localized at nuclear speckles and interacts with nuclear speckle factors involved in RNA splicing and nuclear export, including SRSF1 and Aly/Ref.
Tue Jun 21 00:00:00 EDT 2022
Inhibition of Metastasis by Polypyridyl Ru(II) Complexes through Modification of Cancer Cell Adhesion - In Vitro Functional and Molecular Studies.
Journal of medicinal chemistry
Gurgul I,Janczy-Cempa E,Mazuryk O,Lekka M,Łomzik M,Suzenet F,Gros PC,Brindell M
AM4302 was used in Western Blotting to assess the impact of polypyridyl Ru(II) complexes on various aspects of cancer cell behavior, including inhibiting cell detachment, migration, invasion, transmigration, re-adhesion, and increasing cell elasticity, while also affecting the activity of integrins and upregulating the expression of focal adhesion components.
Thu Aug 11 00:00:00 EDT 2022
The novel anti-colitic effect of β-adrenergic receptors via modulation of PS1/BACE-1/Aβ axis and NOTCH signaling in an ulcerative colitis model.
Frontiers in pharmacology
Nasser S,Abdallah DM,Ahmed KA,Abdel-Mottaleb Y,El-Abhar HS
AM4302 was used in Western Blotting to investigate the impact of mirabegron (β3-agonist) and/or carvedilol (β1/β2 antagonist) on iodoacetamide-induced ulcerative colitis with emphasis on the novel pathomechanism of the PS1/BACE-1/Aβ axis in ulcerative colitis, and its relation to the inflammatory cascade, fibrotic processes, and the gut barrier dysfunction.
Fri Nov 18 00:00:00 EST 2022
A Series of BRAF- and NRAS-Driven Murine Melanoma Cell Lines with Inducible Gene Modulation Capabilities.
JID innovations : skin science from molecules to population health
Bok I,Angarita A,Douglass SM,Weeraratna AT,Karreth FA
AM4302 was used in Western Blot to established and characterized 21 melanoma cell lines driven by alleles that model the most frequent genetic alterations in human melanoma.
Tue Mar 01 00:00:00 EST 2022
Lymphoedema conditions disrupt endothelial barrier function in vitro.
Journal of the Royal Society, Interface
Hall JD,Farzaneh S,Babakhani Galangashi R,Pujari A,Sweet DT,Kahn ML,Jiménez JM
AM4302 was used in Western Blotting to characterize the lymphatic flow field of a platelet-specific receptor C-type lectin-like receptor 2 (CLEC2) deficient lymphoedema mouse model.
Mon Aug 01 00:00:00 EDT 2022
Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis.
Cell metabolism
Kang YP,Mockabee-Macias A,Jiang C,Falzone A,Prieto-Farigua N,Stone E,Harris IS,DeNicola GM
AM4302 was used in Western Blot to understand the metabolic consequences of cystine starvation in non-small cell lung cancer cells.
Tue Jan 05 00:00:00 EST 2021
LXH254, a Potent and Selective ARAF-Sparing Inhibitor of BRAF and CRAF for the Treatment of MAPK-Driven Tumors.
Clinical cancer research : an official journal of the American Association for Cancer Research
Monaco KA,Delach S,Yuan J,Mishina Y,Fordjour P,Labrot E,McKay D,Guo R,Higgins S,Wang HQ,Liang J,Bui K,Green J,Aspesi P,Ambrose J,Mapa F,Griner L,Jaskelioff M,Fuller J,Crawford K,Pardee G,Widger S,Hammerman PS,Engelman JA,Stuart DD,Cooke VG,Caponigro G
AM4302 was used in Western Blot to describe in detail novel properties of the type II RAF inhibitor, LXH254.
Thu Apr 01 00:00:00 EDT 2021
Up-regulation of gasdermin C in mouse small intestine is associated with lytic cell death in enterocytes in worm-induced type 2 immunity.
Proceedings of the National Academy of Sciences of the United States of America
Xi R,Montague J,Lin X,Lu C,Lei W,Tanaka K,Zhang YV,Xu X,Zheng X,Zhou X,Urban JF,Iwatsuki K,Margolskee RF,Matsumoto I,Tizzano M,Li J,Jiang P
AM4302 was used in Immunohistochemistry to propose that the up-regulated Gsdmc family may be major effectors for type 2 responses in the gut and that Gsdmc-mediated pyroptosis may provide a conduit for the release of antiparasitic factors from enterocytes to facilitate the clearance of worms.
Tue Jul 27 00:00:00 EDT 2021
Pseudomonas aeruginosa survives in epithelia by ExoS-mediated inhibition of autophagy and mTOR.
EMBO reports
Rao L,De La Rosa I,Xu Y,Sha Y,Bhattacharya A,Holtzman MJ,Gilbert BE,Eissa NT
AM4302 was used in Western Blot to show that the type III secretion system effector protein ExoS facilitates P. aeruginosa survival in airway epithelial cells by inhibiting autophagy in host cells.
Wed Feb 03 00:00:00 EST 2021
SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.
Journal of virology
Chen DY,Khan N,Close BJ,Goel RK,Blum B,Tavares AH,Kenney D,Conway HL,Ewoldt JK,Chitalia VC,Crossland NA,Chen CS,Kotton DN,Baker SC,Fuchs SY,Connor JH,Douam F,Emili A,Saeed M
AM4302 was used in Western Blot to generate a panel of phenotypically diverse, SARS-CoV-2-infectible human cell lines representing different body organs and performed longitudinal survey of cellular proteins and pathways broadly affected by the virus.
Thu Sep 09 00:00:00 EDT 2021
MAGI-1 PDZ2 Domain Blockade Averts Adenovirus Infection via Enhanced Proteolysis of the Apical Coxsackievirus and Adenovirus Receptor.
Journal of virology
Alghamri MS,Sharma P,Williamson TL,Readler JM,Yan R,Rider SD,Hostetler HA,Cool DR,Kolawole AO,Excoffon KJDA
AM4302 was used in Western Blot to show that the interactions between the MAGI-1 PDZ2 domain and CAREx8 would decrease the apical CAREx8 expression level and prevent AdV infection.
Thu Jun 10 00:00:00 EDT 2021
Low-Density Lipoprotein Receptor Suppresses the Endogenous Cholesterol Synthesis Pathway To Oppose Gammaherpesvirus Replication in Primary Macrophages.
Journal of virology
Aurubin CA,Knaack DA,Sahoo D,Tarakanova VL
AM4302 was used in Western Blot to show that LDL-R protein levels are decreased in infected cells to mitigate the antiviral effects, revealing an intriguing tug of war between the virus and the host.
Tue Aug 10 00:00:00 EDT 2021
Functional expression of complement factor I following AAV-mediated gene delivery in the retina of mice and human cells.
Gene therapy
Dreismann AK,McClements ME,Barnard AR,Orhan E,Hughes JP,Lachmann PJ,MacLaren RE
AM4302 was used in Western Blot to demonstrate the generation of AAV constructs carrying the human CFI sequence and expression of CFI in cell lines and in the retina of C57BL/6 J mice.
Sat May 01 00:00:00 EDT 2021
LncRNA MNX1-AS1 Contributes to Laryngeal Squamous Cell Carcinoma Growth and Migration by Regulating mir-744-5p/bcl9/β-Catenin Axis.
Cell transplantation
Ma B,Ren G,Xu J,Yin C,Shi Y
AM4302 was used in Western Blot to disclose the role of MNX1-AS1 in LSCC progression, and explore whether MNX1-AS1 participates in LSCC progression via targeting miR-744-5p to active BCL9/β-catenin signaling.
Wed Nov 17 00:00:00 EST 2021
Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy.
American journal of translational research
Cai M,Du B,Si Y,Miao J,Ge J,Zhang J,Song J,Bao H
AM4302 was used in Western Blot to elucidate the underlying mechanisms where knockdown of voltage dependent anion channel (VDAC1) alleviates the cognitive dysfunction caused by sepsis-associated encephalopathy.
Thu Nov 18 00:00:00 EST 2021
CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs.
Molecular medicine reports
Xian W,Wu J,Li Q,Du X,Wang N,Chen D,Gao W,Cao J
AM4302 was used in Western Blot to elucidate whether overexpressed C‚X‚C motif chemokine receptor 3 affects regulatory T cells and consequently reduces renal ischemia‚reperfusion injury.
Thu Jul 01 00:00:00 EDT 2021
Identification of Nucleolin as a Novel AEG-1-Interacting Protein in Breast Cancer via Interactome Profiling.
Cancers
Lee SJ,Choi KM,Bang G,Park SG,Kim EB,Choi JW,Chung YH,Kim J,Lee SG,Kim E,Kim JY
AM4302 was used in Western Blot to identify NCL as a novel mediator of the oncogenic function of AEG-1, and suggest that c-Met could be associated with the oncogenic function of the AEG-1-NCL complex in the context of breast cancer.
Mon Jun 07 00:00:00 EDT 2021
Glycogen Synthase Kinase 3 Beta Regulates the Human Aryl Hydrocarbon Receptor Cellular Content and Activity.
International journal of molecular sciences
Yang Y,Chan WK
AM4302 was used in Western Blot to investigate how the aryl hydrocarbon receptor undergoes protein degradation and how its activity is modulated using HeLa cells.
Sat Jun 05 00:00:00 EDT 2021
The Aryl Hydrocarbon Receptor Undergoes Chaperone-Mediated Autophagy in Triple-Negative Breast Cancer Cells.
International journal of molecular sciences
Chen J,Yang Y,Russu WA,Chan WK
AM4302 was used in Western Blotting to report that AHR undergoes chaperone-mediated autophagy in MDA-MB-468 triple-negative breast cancer cells.
Sat Feb 06 00:00:00 EST 2021
RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.
Cell
Rheinemann L,Downhour DM,Bredbenner K,Mercenne G,Davenport KA,Schmitt PT,Necessary CR,McCullough J,Schmitt AP,Simon SM,Sundquist WI,Elde NC
AM4302 was used in Western Blotting to describe duplicated and truncated copies of the ESCRT-III factor CHMP3 that block ESCRT-dependent virus budding and arose independently in New World monkeys and mice.
Thu Oct 14 00:00:00 EDT 2021
The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction.
Biomolecules
Wanschel ACBA,Guizoni DM,Lorza-Gil E,Salerno AG,Paiva AA,Dorighello GG,Davel AP,Balkan W,Hare JM,Oliveira HCF
AM4302 was used in Western Blotting to support the notion that CETP expression negatively impacts endothelial cell function, revealing a new mechanism that might contribute to atherosclerosis.
Thu Jan 07 00:00:00 EST 2021
Melatonin Reduces Oxidative Stress in the Right Ventricle of Newborn Sheep Gestated under Chronic Hypoxia.
Antioxidants (Basel, Switzerland)
Gonzaléz-Candia A,Arias PV,Aguilar SA,Figueroa EG,Reyes RV,Ebensperger G,Llanos AJ,Herrera EA
AM4302 was used in Western Blot to assess whether melatonin improves cardiac function and modulates right ventricle (RV) oxidative stress.
Fri Oct 22 00:00:00 EDT 2021
Expression of Rab Prenylation Pathway Genes and Relation to Disease Progression in Choroideremia.
Translational vision science & technology
Fry LE,Patrício MI,Jolly JK,Xue K,MacLaren RE
AM4302 was used in Western Blotting to investigate whether the transcription and expression of other genes involved in the prenylation of Rab proteins correlates with disease progression in a cohort of patients with choroideremia.
Thu Jul 01 00:00:00 EDT 2021
Interleukin-6 promotes microtubule stability in axons via Stat3 protein-protein interactions.
iScience
Wareham LK,Echevarria FD,Sousa JL,Konlian DO,Dallas G,Formichella CR,Sankaran P,Goralski PJ,Gustafson JR,Sappington RM
AM4302 was used in Immunohistochemistry, Immunocytochemistry, Western Blot to investigate IL-6 in STAT3 in axonal development and repair, and associated changes in microtubules.
Fri Oct 22 00:00:00 EDT 2021
Pulmonary ACE2 expression in neonatal and adult rats.
FEBS open bio
Zhao D,Chen X,Han D,Zhong J,Zhang SE,Yang C
AM4302 was used in Western Blot to investigate whether differences exist in the level of expression and distribution of angiotensin-converting enzyme 2 between neonatal and adult rat lungs.
Sun Aug 01 00:00:00 EDT 2021
Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity.
Molecular autism
Sarn N,Thacker S,Lee H,Eng C
AM4302 was used in Western Blot to explore the effects of PTEN localization on different neurophenotypes.
Fri Jun 04 00:00:00 EDT 2021
Geranylgeranylacetone attenuates cerebral ischemia-reperfusion injury in rats through the augmentation of HSP 27 phosphorylation: a preliminary study.
BMC neuroscience
Matsuo K,Hosoda K,Tanaka J,Yamamoto Y,Imahori T,Nakai T,Irino Y,Shinohara M,Sasayama T,Kohmura E
AM4302 was used in Western Blot to suggest that geranylgeranyl acetone may be a promising agent for the treatment of ischemic stroke and would be worthy of further study.
Mon Feb 08 00:00:00 EST 2021
p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation.
Cell reports
Kilgas S,Singh AN,Paillas S,Then CK,Torrecilla I,Nicholson J,Browning L,Vendrell I,Konietzny R,Kessler BM,Kiltie AE,Ramadan K
AM4302 was used in Western Blotting to find that p97 physically and functionally interacts with the MRE11-RAD50-NBS1 (MRN) complex on chromatin and that inactivation of p97 blocks the disassembly of the MRN complex from the sites of DNA damage upon ionizing radiation (IR).
Tue May 25 00:00:00 EDT 2021
Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents.
Pharmaceuticals (Basel, Switzerland)
Gajda-Morszewski P,Gurgul I,Janczy-Cempa E,Mazuryk O,Łomzik M,Brindell M
AM4302 was used in Western Blotting to show a reduction of metastatic cells escaping from the primary lesion to the surrounding tissue by prevention of their detachment and by influencing the activity of MMP-2 and MMP-9.
Fri Oct 01 00:00:00 EDT 2021
siRNA Knockdown of REDD1 Facilitates Aspirin-Mediated Dephosphorylation of mTORC1 Target 4E-BP1 in MDA-MB-468 Human Breast Cancer Cell Line.
Cancer management and research
Savukaitytė A,Gudoitytė G,Bartnykaitė A,Ugenskienė R,Juozaitytė E
AM4302 was used in Western Blot to suggest that REDD1 downregulation might improve the anticancer activity of aspirin in a subset of breast tumours.
Wed Apr 20 00:00:00 EDT 2022
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability.
Cell reports
Ruggiano A,Vaz B,Kilgas S,Popović M,Rodriguez-Berriguete G,Singh AN,Higgins GS,Kiltie AE,Ramadan K
AM4302 was used in Western Blotting to identify ubiquitylation and SUMOylation as two major signaling events in DNA replication-coupled DNA-protein crosslinks repair.
Tue Dec 07 00:00:00 EST 2021
The protein kinase Akt acts as a coat adaptor in endocytic recycling.
Nature cell biology
Hsu JW,Bai M,Li K,Yang JS,Chu N,Cole PA,Eck MJ,Li J,Hsu VW
AM4302 was used in Western Blotting to show that the protein kinase Akt acts as a co-adaptor in this complex, and is needed in conjunction with ACAP1 to bind to cargo proteins to promote their recycling.
Sat Aug 01 00:00:00 EDT 2020
Cell-Based Phenotypic Drug Screening Identifies Luteolin as Candidate Therapeutic for Nephropathic Cystinosis.
Journal of the American Society of Nephrology : JASN
De Leo E,Elmonem MA,Berlingerio SP,Berquez M,Festa BP,Raso R,Bellomo F,Starborg T,Janssen MJ,Abbaszadeh Z,Cairoli S,Goffredo BM,Masereeuw R,Devuyst O,Lowe M,Levtchenko E,Luciani A,Emma F,Rega LR
AM4302 was used in Western Blot to identify luteolin as a restorer of normal autophagy defective in cystinosis.
Wed Jul 01 00:00:00 EDT 2020
Context-dependent activation of SIRT3 is necessary for anchorage-independent survival and metastasis of ovarian cancer cells.
Oncogene
Kim YS,Gupta Vallur P,Jones VM,Worley BL,Shimko S,Shin DH,Crawford LC,Chen CW,Aird KM,Abraham T,Shepherd TG,Warrick JI,Lee NY,Phaeton R,Mythreye K,Hempel N
AM4302 was used in Western Blotting to highlight the novel context-specific, pro-metastatic role of SIRT3 in ovarian cancer.
Sat Feb 01 00:00:00 EST 2020
Association of Messenger RNA Level With Phenotype in Patients With Choroideremia: Potential Implications for Gene Therapy Dose.
JAMA ophthalmology
Fry LE,Patrício MI,Williams J,Aylward JW,Hewitt H,Clouston P,Xue K,Barnard AR,MacLaren RE
AM4302 was used in Immunocytochemistry to demonstrate the first genotype-phenotype association in choroideremia.
Sat Feb 01 00:00:00 EST 2020
Interplay of ERα binding and DNA methylation in the intron-2 determines the expression and estrogen regulation of cystatin A in breast cancer cells.
Molecular and cellular endocrinology
John Mary DJS,Sikarwar G,Kumar A,Limaye AM
AM4302 was used in Western Blot to investigate the relationship between Cystatin A expression and DNA methylation in 97 specific regions within the CSTA locus in breast cancer cell lines, and breast tumors of the TCGA cohort.
Sun Mar 15 00:00:00 EDT 2020
HSPA12B Secreted by Tumor-Associated Endothelial Cells Might Induce M2 Polarization of Macrophages via Activating PI3K/Akt/mTOR Signaling.
OncoTargets and therapy
Zhou J,Zhang A,Fan L
AM4302 was used in Western Blotting to speculate that aberrantly expressed and secreted HSPA12B by TECs could be taken by macrophages partly via OLR1, leading to subsequent activation of the PI3K/Akt/mTOR signaling pathway and elevated expression of M2 markers.
Sun Apr 17 00:00:00 EDT 2022
Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers.
OncoTargets and therapy
Chintala S,Levan J,Robinson K,Quist K,Katzenellenbogen RA
AM4302 was used in Western Blotting to determine the expression of NFX1-123 in cervical cancer and what genes regulate this.
Fri Apr 15 00:00:00 EDT 2022
Dose and duration of interferon γ pre-licensing interact with donor characteristics to influence the expression and function of indoleamine-2,3-dioxygenase in mesenchymal stromal cells.
Journal of the Royal Society, Interface
Boyt DT,Boland LK,Burand AJ,Brown AJ,Ankrum JA
AM4302 was used in Western Blotting to systematically examine the relative role that the dose of IFNγ, the duration of pre-licensing and the donor of origin play in dictating MSC production of functional IDO.
Mon Jun 01 00:00:00 EDT 2020
Eugenol attenuates inflammatory response and enhances barrier function during lipopolysaccharide-induced inflammation in the porcine intestinal epithelial cells.
Journal of animal science
Hui Q,Ammeter E,Liu S,Yang R,Lu P,Lahaye L,Yang C
AM4302 was used in Western Blot to a low dose of eugenol attenuated inflammatory responses and enhanced selectively permeable barrier function during LPS-induced inflammation in the IPEC-J2 cell line.
Sat Aug 01 00:00:00 EDT 2020
Generation of genomic-integration-free human induced pluripotent stem cells and the derived cardiomyocytes of X-linked dilated cardiomyopathy from DMD gene mutation.
Stem cell research
Zhu S,Law AHY,Deng R,Poon ENY,Lo CW,Kwong AKY,Liang R,Chan KYK,Wong WL,Tan-Un KC,Pijnappel WWMP,Chan GCF,Chan SHS
AM4302 was used in Western Blotting to derived an integration-free induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells (PBMCs) of a 23-year-old male patient.
Tue Dec 01 00:00:00 EST 2020
PHGDH supports liver ceramide synthesis and sustains lipid homeostasis.
Cancer & metabolism
Kang YP,Falzone A,Liu M,González-Sánchez P,Choi BH,Coloff JL,Saller JJ,Karreth FA,DeNicola GM
AM4302 was used in Western Blotting to study PHGDH inhibition in adult animals using a knock-in mouse model.
Fri Apr 15 00:00:00 EDT 2022
MINPP1 prevents intracellular accumulation of the chelator inositol hexakisphosphate and is mutated in Pontocerebellar Hypoplasia.
Nature communications
Ucuncu E,Rajamani K,Wilson MSC,Medina-Cano D,Altin N,David P,Barcia G,Lefort N,Banal C,Vasilache-Dangles MT,Pitelet G,Lorino E,Rabasse N,Bieth E,Zaki MS,Topcu M,Sonmez FM,Musaev D,Stanley V,Bole-Feysot C,Nitschké P,Munnich A,Bahi-Buisson N,Fossoud C,Giuliano F,Colleaux L,Burglen L,Gleeson JG,Boddaert N,Saiardi A,Cantagrel V
AM4302 was used in Western Blotting to describe an early-onset neurodegenerative syndrome caused by loss-of-function mutations in the multiple inositol-polyphosphate phosphatase 1 gene (MINPP1).
Mon Nov 30 00:00:00 EST 2020
HIV Tat-mediated induction of autophagy regulates the disruption of ZO-1 in brain endothelial cells.
Tissue barriers
Liao K,Niu F,Hu G,Guo ML,Sil S,Buch S
AM4302 was used in Western Blotting to identify autophagy as an important mechanism underlying Tat-mediated disruption of the BBB.
Thu Apr 02 00:00:00 EDT 2020
Inclusion of PF68 Surfactant Improves Stability of rAAV Titer when Passed through a Surgical Device Used in Retinal Gene Therapy.
Molecular therapy. Methods & clinical development
Patrício MI,Cox CI,Blue C,Barnard AR,Martinez-Fernandez de la Camara C,MacLaren RE
AM4302 was used in Western Blotting to assess the biocompatibility and stability of rAAV2-REP1 (Rab Escort Protein-1) in a mock clinical scenario.
Fri Jun 12 00:00:00 EDT 2020
Dynamic and regulated TAF gene expression during mouse embryonic germ cell development.
PLoS genetics
Gura MA,Mikedis MM,Seymour KA,de Rooij DG,Page DC,Freiman RN
AM4302 was used in Western Blot to study the transcriptional regulation underlying embryonic development including epigenetic reprogramming, quiescence, and meiosis.
Wed Jan 01 00:00:00 EST 2020
KRAS K104 modification affects the KRASG12D-GEF interaction and mediates cell growth and motility.
Scientific reports
Chen CC,Hsu CY,Lin HY,Zeng HQ,Cheng KH,Wu CW,Tsai EM,Hsieh TH
AM4302 was used in Western Blotting to investigate the regulatory role of KRAS K104 modification on the KRASG12D mutant.
Thu Oct 15 00:00:00 EDT 2020
Certain ortho-hydroxylated brominated ethers are promiscuous kinase inhibitors that impair neuronal signaling and neurodevelopmental processes.
The Journal of biological chemistry
Poston RG,Murphy L,Rejepova A,Ghaninejad-Esfahani M,Segales J,Mulligan K,Saha RN
AM4302 was used in Western Blotting to support that certain ortho-hydroxylated PBDE metabolites are promiscuous kinase inhibitors and can cause disruptions of critical neurodevelopmental processes.
Fri May 01 00:00:00 EDT 2020
The Hepatitis B Virus Pre-Core Protein p22 Activates Wnt Signaling.
Cancers
Tran BM,Flanagan DJ,Ebert G,Warner N,Tran H,Fifis T,Kastrappis G,Christophi C,Pellegrini M,Torresi J,Phesse TJ,Vincan E
AM4302 was used in Western Blotting to investigate the interaction between Wnt-signaling and hepatitis B virus infection.
Sun May 31 00:00:00 EDT 2020
SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.
bioRxiv : the preprint server for biology
Chen DY,Khan N,Close BJ,Goel RK,Blum B,Tavares AH,Kenney D,Conway HL,Ewoldt JK,Kapell S,Chitalia VC,Crossland NA,Chen CS,Kotton DN,Baker SC,Connor JH,Douam F,Emili A,Saeed M
AM4302 was used in Flow Cytometry to indicate that the suppression of interferon signaling is a mechanism widely used by SARS-CoV-2 in diverse tissues to evade antiviral innate immunity, and that targeting the viral mediators of immune evasion may help block virus replication in patients with COVID-19.
Wed Oct 28 00:00:00 EDT 2020
SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.
bioRxiv : the preprint server for biology
Chen DY,Khan N,Close BJ,Goel RK,Blum B,Tavares AH,Kenney D,Conway HL,Ewoldt JK,Kapell S,Chitalia VC,Crossland NA,Chen CS,Kotton DN,Baker SC,Connor JH,Douam F,Emili A,Saeed M
AM4302 was used in Flow Cytometry to indicate that the suppression of interferon signaling is a mechanism widely used by SARS-CoV-2 in diverse tissues to evade antiviral innate immunity, and that targeting the viral mediators of immune evasion may help block virus replication in patients with COVID-19.
Wed Oct 28 00:00:00 EDT 2020
An improved method for high-throughput quantification of autophagy in mammalian cells.
Scientific reports
Koepke L,Winter B,Grenzner A,Regensburger K,Engelhart S,van der Merwe JA,Krebs S,Blum H,Kirchhoff F,Sparrer KMJ
AM4302 was used in Western Blotting to help offer ready-to-use protocols to generate sensitive autophagy reporter cells and quantify autophagy in high-throughput assays.
Wed Jul 22 00:00:00 EDT 2020
Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.
Methods in molecular biology (Clifton, N.J.)
Berardinelli SJ,Haltiwanger RS
AM4302 was used in Western Blotting to describe in detail the methods developed to analyze secretion defects of ADAMTS proteins upon loss of either POFUT2 or B3GLCT.
Fri Apr 10 00:00:00 EDT 2020
4'-Phosphopantetheine corrects CoA, iron, and dopamine metabolic defects in mammalian models of PKAN.
EMBO molecular medicine
Jeong SY,Hogarth P,Placzek A,Gregory AM,Fox R,Zhen D,Hamada J,van der Zwaag M,Lambrechts R,Jin H,Nilsen A,Cobb J,Pham T,Gray N,Ralle M,Duffy M,Schwanemann L,Rai P,Freed A,Wakeman K,Woltjer RL,Sibon OC,Hayflick SJ
AM4302 was used in Western Blotting to present a mouse model of PKAN with CoA, iron, and dopamine metabolic defects that can be specifically attributed to loss of pantothenate kinase 2 function.
Sun Dec 01 00:00:00 EST 2019
CoCl2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis.
Biological research
Rana NK,Singh P,Koch B
AM4302 was used in Western Blotting to investigate the expression pattern of the hypoxia associated genes and their association during breast cancer progression under hypoxic microenvironment in breast cancer cells.
Fri Mar 15 00:00:00 EDT 2019
Antioxidant and immunomodulatory activity induced by stevioside in liver damage: In vivo, in vitro and in silico assays.
Life sciences
Casas-Grajales S,Ramos-Tovar E,Chávez-Estrada E,Alvarez-Suarez D,Hernández-Aquino E,Reyes-Gordillo K,Cerda-García-Rojas CM,Camacho J,Tsutsumi V,Lakshman MR,Muriel P
AM4302 was used in Western Blotting to study whether stevioside has beneficial effects in liver injury induced by long-term thioacetamide (TAA) administration and investigate the possible underlying molecular mechanism using in vivo, in vitro and in silico approaches.
Wed May 01 00:00:00 EDT 2019
Cul3 regulates cyclin E1 protein abundance via a degron located within the N-terminal region of cyclin E.
Journal of cell science
Davidge B,Rebola KGO,Agbor LN,Sigmund CD,Singer JD
AM4302 was used in Immunopurification to describe a mechanism whereby N-terminally truncated cyclin E can avoid the Cul3-mediated degradation pathway.
Wed Nov 06 00:00:00 EST 2019
Thyronamine regulation of TAAR1 expression in breast cancer cells and investigation of its influence on viability and migration.
Breast cancer (Dove Medical Press)
Tremmel E,Hofmann S,Kuhn C,Heidegger H,Heublein S,Hermelink K,Wuerstlein R,Harbeck N,Mayr D,Mahner S,Ditsch N,Jeschke U,Vattai A
AM4302 was used in Western Blot to study the regulation of TAAR1 in breast cancer cells and the influence of thyronamines on its expression.
Fri Apr 08 00:00:00 EDT 2022
An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.
Cellular microbiology
Eshghi A,Gaultney RA,England P,Brûlé S,Miras I,Sato H,Coburn J,Bellalou J,Moriarty TJ,Haouz A,Picardeau M
AM4302 was used in Western Blotting to investigate if LIC10831 elicits an antibody response in infected animals, is actively secreted by the bacterium, and binds human E- and VE-cadherins.
Fri Feb 01 00:00:00 EST 2019
Identification of NFAT5 as a transcriptional regulator of the EDN1 gene in collecting duct.
American journal of physiology. Renal physiology
Lakshmipathi J,Wheatley W,Kumar A,Mercenne G,Rodan AR,Kohan DE
AM4302 was used in Western Blotting to represent the first description that NFAT5 is a direct transcriptional regulator of the EDN1 gene in IMCD cells and point to a potentially important mechanism by which body Na+ homeostasis is maintained.
Fri Mar 01 00:00:00 EST 2019
Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice.
The Biochemical journal
Salerno AG,Rentz T,Dorighello GG,Marques AC,Lorza-Gil E,Wanschel ACBA,de Moraes A,Vercesi AE,Oliveira HCF
AM4302 was used in Western Blotting to show that Nnt plays a critical role in the maintenance of macrophage redox, inflammatory and cholesterol homeostasis, which is relevant for delaying the atherogenesis process.
Mon Dec 23 00:00:00 EST 2019
Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer.
PloS one
Lee KJ,Piett CG,Andrews JF,Mann E,Nagel ZD,Gassman NR
AM4302 was used in Western Blot to evaluate the potential of XRCC1 as an indicator of BER defects in TNBC, we examined XRCC1 expression in the TCGA database and its expression and localization in triple negative breast cancers cell lines.
Mon Mar 09 00:00:00 EDT 2020
Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.
Genome biology
Sharma S,Wang J,Alqassim E,Portwood S,Cortes Gomez E,Maguire O,Basse PH,Wang ES,Segal BH,Baysal BE
AM4302 was used in Western Blotting to study the physiological significance of the APOBEC3A-mediated editing of many mRNAs in monocytes and macrophages in response to hypoxia and interferons.
Thu Feb 21 00:00:00 EST 2019
Determination and analysis of agonist and antagonist potential of naturally occurring flavonoids for estrogen receptor (ERα) by various parameters and molecular modelling approach.
Scientific reports
Puranik NV,Srivastava P,Bhatt G,John Mary DJS,Limaye AM,Sivaraman J
AM4302 was used in Western Blotting to study the effect of the flavonoids on viability, progesterone receptor expression and 3xERE/3XERRE-driven reporter gene expression in ERα positive and estrogen responsive MCF-7 breast cancer cells.
Wed May 15 00:00:00 EDT 2019
Stevia prevents experimental cirrhosis by reducing hepatic myofibroblasts and modulating molecular profibrotic pathways.
Hepatology research : the official journal of the Japan Society of Hepatology
Ramos-Tovar E,Buendia-Montaño LD,Galindo-Gómez S,Hernández-Aquino E,Tsutsumi V,Muriel P
AM4302 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to explore the action mechanism involved.
Fri Feb 01 00:00:00 EST 2019
Stevia prevents experimental cirrhosis by reducing hepatic myofibroblasts and modulating molecular profibrotic pathways.
Hepatology research : the official journal of the Japan Society of Hepatology
Ramos-Tovar E,Buendia-Montaño LD,Galindo-Gómez S,Hernández-Aquino E,Tsutsumi V,Muriel P
AM4302 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to explore the action mechanism involved.
Fri Feb 01 00:00:00 EST 2019
Hyperosmotic stress induces cell-dependent aggregation of α-synuclein.
Scientific reports
Fragniere AMC,Stott SRW,Fazal SV,Andreasen M,Scott K,Barker RA
AM4302 was used in Western Blotting to study the role of hyperosmotic stress in inducing human α-synuclein to aggregate in cells in vitro, through rapid treatment of the cells with three different osmolytes.
Tue Feb 19 00:00:00 EST 2019
TRAF6 Plays a Proviral Role in Tick-Borne Flavivirus Infection through Interaction with the NS3 Protease.
iScience
Youseff BH,Brewer TG,McNally KL,Izuogu AO,Lubick KJ,Presloid JB,Alqahtani S,Chattopadhyay S,Best SM,Hu X,Taylor RT
AM4302 was used in Immunocytochemistry to analyse tick borne flavivirus polyproteins in silico for antiviral protein binding motifs to identify therapeutic targets.
Fri May 31 00:00:00 EDT 2019
Rebaudioside A administration prevents experimental liver fibrosis: an in vivo and in vitro study of the mechanisms of action involved.
Journal of applied toxicology : JAT
Casas-Grajales S,Reyes-Gordillo K,Cerda-García-Rojas CM,Tsutsumi V,Lakshman MR,Muriel P
AM4302 was used in Immunocytochemistry, Western Blot to suggest that Reb A prevents liver damage by blocking oxidative processes via upregulation of nuclear erythroid factor 2, exerts immunomodulatory effects by downregulating the nuclear factor-κB system and acts as an antifibrotic agent by maintaining collagen content.
Thu Aug 01 00:00:00 EDT 2019
Analysis of Vascular Hydrogen Sulfide Biosynthesis.
Methods in molecular biology (Clifton, N.J.)
Lechuga TJ,Chen DB
AM4302 was used in Western Blotting to describe a series of methods that have been optimized for analyzing vascular H2S biosynthesis.
Thu Mar 12 00:00:00 EDT 2020
ALDH7A1 inhibits the intracellular transport pathways during hypoxia and starvation to promote cellular energy homeostasis.
Nature communications
Yang JS,Hsu JW,Park SY,Lee SY,Li J,Bai M,Alves C,Tseng W,Michelet X,Ho IC,Hsu VW
AM4302 was used in Western Blotting to advance the understanding of intracellular transport by revealing how the coordination of multiple pathways can be achieved and demonstrate the role of NADH in cellular energetics.
Fri Sep 06 00:00:00 EDT 2019
Downregulation of SNCA Expression by Targeted Editing of DNA Methylation: A Potential Strategy for Precision Therapy in PD.
Molecular therapy : the journal of the American Society of Gene Therapy
Kantor B,Tagliafierro L,Gu J,Zamora ME,Ilich E,Grenier C,Huang ZY,Murphy S,Chiba-Falek O
AM4302 was used in Western Blotting to establish that DNA hypermethylation at SNCA intron 1 allows an effective and sufficient tight downregulation of SNCA expression levels, suggesting the potential of this target sequence combined with the CRISPR-dCas9 technology as a novel epigenetic-based therapeutic approach for PD.
Wed Nov 07 00:00:00 EST 2018
Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury.
Asian journal of andrology
Lee JW,Lee DH,Park JK,Han JS
AM4302 was used in Western Blotting to investigate whether nitric oxide-derived from nitrite is beneficial in a rat model of testicular ischaemia/reperfusion injury which eventually results in impaired spermatogenesis.
Fri Oct 12 00:00:00 EDT 2018
Nephron prorenin receptor deficiency alters renal medullary endothelin-1 and endothelin receptor expression.
Physiological research
Ramkumar N,Stuart D,Abraham N,Kohan DE
AM4302 was used in Western Blotting to examine the effects of deleting collecting duct renin or nephron prorenin/renin/prorenin receptor on CD endothelin system components.
Wed Jun 27 00:00:00 EDT 2018
Double-stranded RNA innate immune response activation from long-term adeno-associated virus vector transduction.
JCI insight
Shao W,Earley LF,Chai Z,Chen X,Sun J,He T,Deng M,Hirsch ML,Ting J,Samulski RJ,Li C
AM4302 was used in Western Blotting to study the role of the innate immune response at the later time points following adeno-associated viral vector transduction.
Thu Jun 21 00:00:00 EDT 2018
Oligomannosidic glycans at Asn-110 are essential for secretion of human diamine oxidase.
The Journal of biological chemistry
Gludovacz E,Maresch D,Lopes de Carvalho L,Puxbaum V,Baier LJ,Sützl L,Guédez G,Grünwald-Gruber C,Ulm B,Pils S,Ristl R,Altmann F,Jilma B,Salminen TA,Borth N,Boehm T
AM4302 was used in Western Blotting to study the role of oligomannosidic glycans at Asn-110 in the secretion of human diamine oxidase.
Fri Jan 19 00:00:00 EST 2018
HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction.
Nature communications
Akiyama H,Miller CM,Ettinger CR,Belkina AC,Snyder-Cappione JE,Gummuluru S
AM4302 was used in Western Blotting to study the causative mechanisms for persistent type I interferon signalling in HIV-1 infected individuals.
Mon Aug 27 00:00:00 EDT 2018
Ex Vivo Enteroids Recapitulate In Vivo Citrulline Production in Mice.
The Journal of nutrition
Wang X,Yuan Y,Didelija IC,Mohammad MA,Marini JC
AM4302 was used in Immunohistochemistry, Immunocytochemistry, Western Blot to study the regulation of citrulline production in a highly manipulable system.
Sat Sep 01 00:00:00 EDT 2018
High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.
eLife
Medina-Cano D,Ucuncu E,Nguyen LS,Nicouleau M,Lipecka J,Bizot JC,Thiel C,Foulquier F,Lefort N,Faivre-Sarrailh C,Colleaux L,Guerrera IC,Cantagrel V
AM4302 was used in Western Blotting to study the connection between N-glycan multiplicity and fine-tuned neural cell adhesion during mammalian brain development.
Fri Oct 12 00:00:00 EDT 2018
The Biological Activity of AAV Vectors for Choroideremia Gene Therapy Can Be Measured by In Vitro Prenylation of RAB6A.
Molecular therapy. Methods & clinical development
Patrício MI,Barnard AR,Cox CI,Blue C,MacLaren RE
AM4302 was used in Western Blotting to compare the response of RAB27A and RAB6A to the incorporation of a biotinylated lipid donor in a prenylation reaction in vitro.
Fri Jun 15 00:00:00 EDT 2018
Collecting duct principal, but not intercalated, cell prorenin receptor regulates renal sodium and water excretion.
American journal of physiology. Renal physiology
Ramkumar N,Stuart D,Mironova E,Abraham N,Gao Y,Wang S,Lakshmipathi J,Stockand JD,Kohan DE
AM4302 was used in Western Blotting to indicate that PC, but not IC, PRR modulates ENaC activity, urinary Na+ excretion, and water transport.
Sat Sep 01 00:00:00 EDT 2018
Glycation potentiates α-synuclein-associated neurodegeneration in synucleinopathies.
Brain : a journal of neurology
Vicente Miranda H,Szego ÉM,Oliveira LMA,Breda C,Darendelioglu E,de Oliveira RM,Ferreira DG,Gomes MA,Rott R,Oliveira M,Munari F,Enguita FJ,Simões T,Rodrigues EF,Heinrich M,Martins IC,Zamolo I,Riess O,Cordeiro C,Ponces-Freire A,Lashuel HA,Santos NC,Lopes LV,Xiang W,Jovin TM,Penque D,Engelender S,Zweckstetter M,Klucken J,Giorgini F,Quintas A,Outeiro TF
AM4302 was used in Western Blotting to demonstrate glycation constitutes a novel drug target that can be explored in synucleinopathies as well as in other neurodegenerative conditions.
Mon May 01 00:00:00 EDT 2017
Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.
Developmental cell
Bajikar SS,Wang CC,Borten MA,Pereira EJ,Atkins KA,Janes KA
AM4302 was used in Western Blotting to study the tumour-suppressive mode of action of growth-differentiation factor 11, and an unusual mechanism of its inactivation in triple negative breast cancer.
Mon Nov 20 00:00:00 EST 2017
Codon-Optimized RPGR Improves Stability and Efficacy of AAV8 Gene Therapy in Two Mouse Models of X-Linked Retinitis Pigmentosa.
Molecular therapy : the journal of the American Society of Gene Therapy
Fischer MD,McClements ME,Martinez-Fernandez de la Camara C,Bellingrath JS,Dauletbekov D,Ramsden SC,Hickey DG,Barnard AR,MacLaren RE
AM4302 was used in Western Blotting to describe an optimised gene replacement therapy for human X-linked retinitis pigmentosa disease using an adeno-associated viral vector.
Wed Aug 02 00:00:00 EDT 2017
The mechanism of sirtuin 2-mediated exacerbation of alpha-synuclein toxicity in models of Parkinson disease.
PLoS biology
de Oliveira RM,Vicente Miranda H,Francelle L,Pinho R,Szegö ÉM,Martinho R,Munari F,Lázaro DF,Moniot S,Guerreiro P,Fonseca-Ornelas L,Marijanovic Z,Antas P,Gerhardt E,Enguita FJ,Fauvet B,Penque D,Pais TF,Tong Q,Becker S,Kügler S,Lashuel HA,Steegborn C,Zweckstetter M,Outeiro TF
AM4302 was used in western blot to analyze the mechanistic relationship between sirtuin 2 and alpha-synuclein in Parkinson's disease
Wed Mar 01 00:00:00 EST 2017
BRAF-inhibitor Associated MEK Mutations Increase RAF-Dependent and -Independent Enzymatic Activity.
Molecular cancer research : MCR
Emery CM,Monaco KA,Wang P,Balak M,Freeman A,Meltzer J,Delach SM,Rakiec D,Ruddy DA,Korn JM,Haling J,Acker MG,Caponigro G
AM4302 was used in Western Blotting to show that alternate modes of target inhibition like ERK inhibition is required to effectively treat tumours harbouring MEK1/2-resistant alleles.
Sun Oct 01 00:00:00 EDT 2017
Mitochondrial complex II regulates a distinct oxygen sensing mechanism in monocytes.
Human molecular genetics
Sharma S,Wang J,Cortes Gomez E,Taggart RT,Baysal BE
AM4302 was used in Western Blotting to test the hypothesis that inhibition of complex II facilitates hypoxic gene expression in monocytes using an integrative experimental approach.
Sat Apr 01 00:00:00 EDT 2017
Brilliant cresyl blue staining does not present cytotoxic effects on human luteinized follicular cells, according to gene/protein expression, as well as to cytotoxicity tests.
Reproductive biology
Alcoba DD,Schneider J,Arruda L,Martiny PB,Capp E,von Eye Corleta H,Brum IS
AM4302 was used in western blot to investigate the effects of Brilliant Cresyl Blue on human follicular cells exposed to Brilliant Cresyl Blue
Wed Mar 01 00:00:00 EST 2017
Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins.
Oncotarget
Mitkevich VA,Burnysheva KM,Petrushanko IY,Adzhubei AA,Schulga AA,Chumakov PM,Makarov AA
AM4302 was used in Western Blotting to determine whether binase increases interferon sensitivity and apoptosis in SiHa cervical cancer cells infected with high-risk human papilloma virus strain 16.
Fri Sep 22 00:00:00 EDT 2017
Interaction of melatonin and gonadotropin-inhibitory hormone on the zebrafish brain-pituitary-reproductive axis.
Molecular reproduction and development
Yumnamcha T,Khan ZA,Rajiv C,Devi SD,Mondal G,Sanjita Devi H,Bharali R,Chattoraj A
AM4302 was used in Western Blotting to demonstrate the role of melatonin in oocyte maturation in the zebrafish (Danio rerio), via the brain-pituitary-reproductive axis, under different photic conditions.
Mon May 01 00:00:00 EDT 2017
High-Order Drug Combinations Are Required to Effectively Kill Colorectal Cancer Cells.
Cancer research
Horn T,Ferretti S,Ebel N,Tam A,Ho S,Harbinski F,Farsidjani A,Zubrowski M,Sellers WR,Schlegel R,Porter D,Morris E,Wuerthner J,Jeay S,Greshock J,Halilovic E,Garraway LA,Caponigro G,Lehár J
AM4302 was used in Western Blotting to systematically investigate the efficacy of combinations of two to six drugs that target critical oncogenic pathways in colorectal cancer cell lines.
Thu Dec 01 00:00:00 EST 2016
A Syndromic Intellectual Disability Disorder Caused by Variants in TELO2, a Gene Encoding a Component of the TTT Complex.
American journal of human genetics
You J,Sobreira NL,Gable DL,Jurgens J,Grange DK,Belnap N,Siniard A,Szelinger S,Schrauwen I,Richholt RF,Vallee SE,Dinulos MBP,Valle D,Armanios M,Hoover-Fong J
AM4302 was used in western blot to elucidate variants in TELO2, a gene encoding a component of the TTT complex, causing a syndromic intellectual disability disorder
Thu May 05 00:00:00 EDT 2016
Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor α.
Molecular and cellular biology
Okumura F,Uematsu K,Byrne SD,Hirano M,Joo-Okumura A,Nishikimi A,Shuin T,Fukui Y,Nakatsukasa K,Kamura T
AM4302 was used in western blot to study pVHL-mediated degradation of B-Myb and hypoxia-inducible factor alpha by parallele regulation of von Hippel-Lindau disease
Wed Jun 15 00:00:00 EDT 2016
Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30.
Data in brief
Manjegowda MC,Gupta PS,Limaye AM
AM4302 was used in Western Blotting to present the methodology used for the generation and purification of rabbit antiserum polyclonal antibody against the N-terminus of human GPR30.
Wed Jun 01 00:00:00 EDT 2016
Glycation potentiates neurodegeneration in models of Huntington's disease.
Scientific reports
Vicente Miranda H,Gomes MA,Branco-Santos J,Breda C,Lázaro DF,Lopes LV,Herrera F,Giorgini F,Outeiro TF
AM4302 was used in Western Blotting to provide evidence that glycation modulates HTT exon-1 aggregation and toxicity, and may constitute a therapeutic target for Huntington's disease.
Fri Nov 18 00:00:00 EST 2016
Mutations in NONO lead to syndromic intellectual disability and inhibitory synaptic defects.
Nature neuroscience
Mircsof D,Langouët M,Rio M,Moutton S,Siquier-Pernet K,Bole-Feysot C,Cagnard N,Nitschke P,Gaspar L,Žnidarič M,Alibeu O,Fritz AK,Wolfer DP,Schröter A,Bosshard G,Rudin M,Koester C,Crestani F,Seebeck P,Boddaert N,Prescott K,Hines R,Moss SJ,Fritschy JM,Munnich A,Amiel J,Brown SA,Tyagarajan SK,Colleaux L
AM4302 was used in western blot to characterize NONO-deficient mice
Tue Dec 01 00:00:00 EST 2015
The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea.
Proceedings of the National Academy of Sciences of the United States of America
Golden EJ,Benito-Gonzalez A,Doetzlhofer A
AM4302 was used in western blot to elucidate the molecular mechanisms involved in the timing and coordination of auditory prosensory proliferation and differentiation.
Tue Jul 21 00:00:00 EDT 2015
An analysis of critical factors for quantitative immunoblotting.
Science signaling
Janes KA
AM4302 was used in western blot to examine an immunoblot-analysis workflow for accuracy and precision
Tue Apr 07 00:00:00 EDT 2015
Estrogen Replacement Therapy in Ovariectomized Nonpregnant Ewes Stimulates Uterine Artery Hydrogen Sulfide Biosynthesis by Selectively Up-Regulating Cystathionine β-Synthase Expression.
Endocrinology
Lechuga TJ,Zhang HH,Sheibani L,Karim M,Jia J,Magness RR,Rosenfeld CR,Chen DB
AM4302 was used in Western Blotting to investigate whether oestrogen replacement therapy selectively stimulates hydrogen sulfide biosynthesis in the uterine artery and other systemic arteries.
Mon Jun 01 00:00:00 EDT 2015
Activation of Adenosine Monophosphate-Activated Protein Kinase Is an Additional Mechanism That Participates in Mediating Inhibitory Actions of Prostaglandin F2Alpha in Mature, but Not Developing, Bovine Corpora Lutea.
Biology of reproduction
Bowdridge EC,Goravanahally MP,Inskeep EK,Flores JA
AM4302 was used in western blot to study the inhibitory action of prostaglandin F2alpha in bovine corpora lutea and its mechanism
Wed Jul 01 00:00:00 EDT 2015
Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.
Cell death & disease
Oliveira LM,Falomir-Lockhart LJ,Botelho MG,Lin KH,Wales P,Koch JC,Gerhardt E,Taschenberger H,Outeiro TF,Lingor P,Schüle B,Arndt-Jovin DJ,Jovin TM
AM4302 was used in western blot to research the role of increased alpha-synuclein due to SNCA gene triplication and its role in Parkinson stem cells
Thu Nov 26 00:00:00 EST 2015
Impact of Diverse Immune Evasion Mechanisms of Cancer Cells on T Cells Engaged by EpCAM/CD3-Bispecific Antibody Construct AMG 110.
PloS one
Deisting W,Raum T,Kufer P,Baeuerle PA,Münz M
AM4302 was used in western blot to study the use of bispecific T cell engager
Thu Jun 16 00:00:00 EDT 2016
Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells.
Journal of stem cell research & therapy
Rutering J,Ilmer M,Recio A,Coleman M,Vykoukal J,Alt E
AM4302 was used in Immunocytochemistry-immunoflourescence to create a protocol for improved isolation of neonatal rat cardiomyocytes that also enables enhanced yields of cardiac progenitor cells.
Tue Sep 29 00:00:00 EDT 2020
Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells.
Journal of stem cell research & therapy
Rutering J,Ilmer M,Recio A,Coleman M,Vykoukal J,Alt E
AM4302 was used in Immunocytochemistry-immunoflourescence to create a protocol for improved isolation of neonatal rat cardiomyocytes that also enables enhanced yields of cardiac progenitor cells.
Tue Sep 29 00:00:00 EDT 2020
Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis.
PloS one
Mello AA,Leal MF,Rey JA,Pinto GR,Lamarão LM,Montenegro RC,Alves AP,Assumpção PP,Borges Bdo N,Smith MC,Burbano RR
AM4302 was used in western blot to assess SRC, LYN, and CKB expression, as well as their promoter methylation, in gastric cancer
Mon Jun 06 00:00:00 EDT 2016
Cyclin C is a haploinsufficient tumour suppressor.
Nature cell biology
Li N,Fassl A,Chick J,Inuzuka H,Li X,Mansour MR,Liu L,Wang H,King B,Shaik S,Gutierrez A,Ordureau A,Otto T,Kreslavsky T,Baitsch L,Bury L,Meyer CA,Ke N,Mulry KA,Kluk MJ,Roy M,Kim S,Zhang X,Geng Y,Zagozdzon A,Jenkinson S,Gale RE,Linch DC,Zhao JJ,Mullighan CG,Harper JW,Aster JC,Aifantis I,von Boehmer H,Gygi SP,Wei W,Look AT,Sicinski P
AM4302 was used in western blot to elucidate the regulation of Notch by Cyclin C.
Sat Nov 01 00:00:00 EDT 2014
Determinants of Moloney murine leukemia virus Gag-Pol and genomic RNA proportions.
Journal of virology
Johnson SF,Collins JT,D'Souza VM,Telesnitsky A
AM4302 was used in western blot to study virion component proportions using moloney murine leukemia virus.
Tue Jul 01 00:00:00 EDT 2014
Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea.
Biology of reproduction
Wright MF,Bowdridge E,McDermott EL,Richardson S,Scheidler J,Syed Q,Bush T,Inskeep EK,Flores JA
AM4302 was used in western blot to study mechanisms of calcium homeostasis in bovine corpora lutea.
Sat Mar 01 00:00:00 EST 2014
Serum stability and physicochemical characterization of a novel amphipathic peptide C6M1 for siRNA delivery.
PloS one
Jafari M,Xu W,Pan R,Sweeting CM,Karunaratne DN,Chen P
AM4302 was used in western blot to investigate the use of peptides as carriers of short interfering RNA.
Mon Jun 15 00:00:00 EDT 2015
Mesenchymal stem cells contribute to the chemoresistance of hepatocellular carcinoma cells in inflammatory environment by inducing autophagy.
Cell & bioscience
Han Z,Jing Y,Xia Y,Zhang S,Hou J,Meng Y,Yu F,Liu X,Wu M,Zhang P,Wei L
AM4302 was used in western blot to examine the role of mesenchymal stem cells in the development of hepatocellular carcinoma resistance to chemotherapy.
Thu May 29 00:00:00 EDT 2014
A noncatalytic function of the ligation complex during nonhomologous end joining.
The Journal of cell biology
Cottarel J,Frit P,Bombarde O,Salles B,Négrel A,Bernard S,Jeggo PA,Lieber MR,Modesti M,Calsou P
AM4302 was used in Western Blotting to elucidate a noncatalytic function of the ligation complex during nonhomologous end joining.
Mon Jan 21 00:00:00 EST 2013
Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators.
Development (Cambridge, England)
Lavado A,He Y,Paré J,Neale G,Olson EN,Giovannini M,Cao X
AM4302 was used in western blot to demonstrate that tumor suppressor neurofibromatosis 2 limits the expansion of neural progenitor cells.
Thu Aug 01 00:00:00 EDT 2013
The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.
Biochimica et biophysica acta
Incontro S,Ciruela F,Ziff E,Hofmann F,Sánchez-Prieto J,Torres M
AM4302 was used in Western Blotting to demonstrate that the type II cGMP-dependent protein kinase phosphorylates GluA1 at S845, augmenting the surface expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors at both synaptic and extrasynaptic sites.
Thu Aug 01 00:00:00 EDT 2013
FcγR-driven release of IL-6 by macrophages requires NOX2-dependent production of reactive oxygen species.
The Journal of biological chemistry
Franchini AM,Hunt D,Melendez JA,Drake JR
AM4302 was used in Western Blotting to elucidate the role of NADPH oxidase-generated reactive oxygen species in macrophage activation, following FcγR engagement.
Fri Aug 30 00:00:00 EDT 2013
Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.
American journal of physiology. Heart and circulatory physiology
Cheung TM,Ganatra MP,Peters EB,Truskey GA
AM4302 was used in western blot to study permeability and tight junctions in endothelial cells derived from human cord blood.
Sat Dec 01 00:00:00 EST 2012
Moloney murine leukemia virus genomic RNA packaged in the absence of a full complement of wild type nucleocapsid protein.
Virology
Johnson SF,Garcia EL,Summers MF,Telesnitsky A
AM4302 was used in western blot to study the role of Gag proteins in gRNA packaging.
Sat Sep 01 00:00:00 EDT 2012
Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress.
The Journal of biological chemistry
Liu XD,Ko S,Xu Y,Fattah EA,Xiang Q,Jagannath C,Ishii T,Komatsu M,Eissa NT
AM4302 was used in western blot to investigate the regulatory mechanisms of aggresome-like induced structures formation and clearance.
Fri Jun 01 00:00:00 EDT 2012
Spatial regulation of interleukin-6 signaling in response to neurodegenerative stressors in the retina.
American journal of neurodegenerative disease
Sims SM,Holmgren L,Cathcart HM,Sappington RM
AM4302 was used in western blot to study the effect of IL-6 on retinal ganglion cells.
Tue Feb 05 00:00:00 EST 2013
Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40.
Analytical biochemistry
Park MS,Chu F,Xie J,Wang Y,Bhattacharya P,Chan WK
AM4302 was used in Western Blotting to identify proteins that interact with cyclophilin-40, elucidating a potential cellular function.
Tue Mar 15 00:00:00 EDT 2011
