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Invitrogen
VISTA Monoclonal Antibody (MIH64), PerCP-eFluor™ 710, eBioscience™
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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VISTA Antibody (46-1083-82) in Flow
Product Details
46-1083-82
Species Reactivity
Host/Isotype
Recommended Isotype Control
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Conjugate
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Product Specific Information
Description: This MIH64 monoclonal antibody recognizes mouse VISTA also known as B7-H5 or PD-H1.
This MIH64 antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells.
Applications Reported: This MIH64 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH64 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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References (0)
Bioinformatics
Protein Aliases: differentiation of ESCs 1; PD-H1; Platelet receptor Gi24; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation
Gene Aliases: Dies1; PD-1H; VISTA; Vsir
UniProt ID: (Mouse) Q9D659
Entrez Gene ID: (Mouse) 74048
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