Invitrogen

TRAIL-R2 (DR5) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

6 References

View all (59) TRAIL-R2 (DR5) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TRAIL-R2 (DR5) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

TRAIL-R2 (DR5) Antibody (PA1-957) in ICC/IF

Immunofluorescence analysis of TRAIL-R2 was performed using HCT 116 cells and HCT 116 treated with Thapsigargin (1 µM, 36 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRAIL-R2 Rabbit Polyclonal Antibody (Product # PA1-957) at 2 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled wi... View More

TRAIL-R2 (DR5) Antibody in Western Blot (WB)

TRAIL-R2 (DR5) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

PA1-957

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-3 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C V(255) P E Q E M E V Q E P A E P T G(270)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2303474

Product Specific Information

PA1-957 detects death receptor 5 (DR5) from human and rat tissues and cells.

PA1-957 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~58 kDa protein representing DR5 from HEK293 cell lysate.

PA1-957 immunizing peptide corresponds to amino acid residues 255-270 of rat DR5. PA1-957 immunizing peptide (Cat. # PEP-048) is available for use in neutralization and control experiments.

Target Information

DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Use of Single Cell Transcriptomic Techniques to Study the Role of High-Risk Human Papillomavirus Infection in Cervical Cancer.

Frontiers in immunology

Meng L,Chen S,Shi G,He S,Wang Z,Shen J,Wang J,Sooranna SR,Zhao J,Song J

PA1-957 was used in Immunohistochemistry (Frozen) to provide a basis for the development of a rational post-operative follow-up program and the development of an appropriate treatment plan for patients with cervical cancer.

Fri Jul 01 00:00:00 EDT 2022

Oxaliplatin resistance in colorectal cancer enhances TRAIL sensitivity via death receptor 4 upregulation and lipid raft localization.

eLife

Greenlee JD,Lopez-Cavestany M,Ortiz-Otero N,Liu K,Subramanian T,Cagir B,King MR

PA1-957 was used in Western Blot to examine whether surviving oxaliplatin-resistant subpopulations of cancer cells have an enhanced sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and elucidate mechanisms that drive this sensitization.

Tue Aug 03 00:00:00 EDT 2021

HIF-1α Mediates TRAIL-Induced Neuronal Apoptosis via Regulating DcR1 Expression Following Traumatic Brain Injury.

Frontiers in cellular neuroscience

Fang Y,Lu J,Wang X,Wu H,Mei S,Zheng J,Xu S,Lenahan C,Chen S,Zhang J,Hong Y

PA1-957 was used in Western Blot, Immunocytochemistry to study the expression and cell localization of tumor necrosis factor-related apoptosis-inducing ligand and death receptor 5 (DR5) after traumatic brain injury in rats.

Mon Sep 28 00:00:00 EDT 2020

HIF-1α Mediates TRAIL-Induced Neuronal Apoptosis via Regulating DcR1 Expression Following Traumatic Brain Injury.

Frontiers in cellular neuroscience

Fang Y,Lu J,Wang X,Wu H,Mei S,Zheng J,Xu S,Lenahan C,Chen S,Zhang J,Hong Y

Mon Sep 28 00:00:00 EDT 2020

BCL2L10 protein regulates apoptosis/proliferation through differential pathways in gastric cancer cells.

The Journal of pathology

Xu JD,Cao XX,Long ZW,Liu XP,Furuya T,Xu JW,Liu XL,De Xu Z,Sasaki K,Li QQ

PA1-957 was used in western blot to investigate the role of BCL2L10 in cell cycle regulation in tumors

Tue Feb 01 00:00:00 EST 2011

Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors.

Science (New York, N.Y.)

Sheridan JP,Marsters SA,Pitti RM,Gurney A,Skubatch M,Baldwin D,Ramakrishnan L,Gray CL,Baker K,Wood WI,Goddard AD,Godowski P,Ashkenazi A

PA1-957 was used in western blot to investigate the regulation of apoptosis induced by TRAIL

Fri Aug 08 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: apoptosis inducing protein TRICK2A/2B; apoptosis inducing receptor TRAIL-R2; CD262; cytotoxic TRAIL receptor-2; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; dr-5; Fas-like protein; KILLER/DR5 TRAIL death-inducing receptor; MK; p53-regulated DNA damage-inducible cell death receptor(killer); sCD262; soluble CD262; TNF-related apoptosis-inducing ligand receptor 2; TRAIL receptor 2; Tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9

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Gene Aliases: CD262; DR5; KILLER; KILLER/DR5; Ly98; MK; TNFRSF10B; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; UNQ160/PRO186; ZTNFR9

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UniProt ID: (Human) O14763, (Mouse) Q9QZM4

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Entrez Gene ID: (Human) 8795, (Mouse) 21933, (Rat) 364420

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Function(s)

receptor activity tumor necrosis factor-activated receptor activity protein binding TRAIL binding protease binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process molecular_function transmembrane signal receptor

Process(es)

apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response immune response cell surface receptor signaling pathway activation of NF-kappaB-inducing kinase activity multicellular organism development extrinsic apoptotic signaling pathway via death domain receptors response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway response to endoplasmic reticulum stress regulation of cell proliferation regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to mechanical stimulus activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors signal transduction TRAIL-activated apoptotic signaling pathway extrinsic apoptotic signaling pathway male gonad development response to organic substance response to organic cyclic compound

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TRAIL-R2 (DR5) Polyclonal Antibody

TRAIL-R2 (DR5) Antibody (PA1-957) in ICC/IF

Product Details

PA1-957

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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