Invitrogen

TRA-1-60 Monoclonal Antibody (TRA-1-60), DyLight™ 550

TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 1

TRA-1-60 Antibody (MA1-023-D550) in ICC/IF

Immunofluorescent analysis of TRA-1-60 (red) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated TRA-1-60 monoclonal antibody (Product # MA1-023-D550) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magni... View More

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Product Details

MA1-023-D550

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TRA-1-60

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

Excitation/Emission Max

556/570 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536701

Product Specific Information

MA1-023-D550 has been successfully used in ICC/IF applications on human samples.

Target Information

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: TRA160; tra60

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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