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STAT5A Polyclonal Antibody

STAT5A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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STAT5A Antibody (13179-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13179-1-AP ( STAT5A antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

STAT5A Antibody in Flow Cytometry (Flow)

STAT5A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13179-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

STAT5A Fusion Protein Ag3846 (445-794 aa encoded by BC027036)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.

Immunogen sequence: ESQFSVGSN ELVFQVKTLS LPVVVIVHGS QDHNATATVL WDNAFAEPGR VPFAVPDKVL WPQLCEALNM KFKAEVQSNR GLTKENLVFL AQKLFNNSSS HLEDYSGLSV SWSQFNRENL PGWNYTFWQW FDGVMEVLKK HHKPHWNDGA ILGFVNKQQA HDLLINKPDG TFLLRFSDSE IGGITIAWKF DSPERNLWNL KPFTTRDFSI RSLADRLGDL SYLIYVFPDR PKDEVFSKYY TPVLAKAVDG YVKPQIKQVV PEFVNASADA GGSSATYMDQ APSPAVCPQA PYNMYPQNPD HVLDQDGEFD LDETMDVARH VEELLRRPMD SLDSRLSPPA GLFTSARGSL S (445-794 aa encoded by BC027036)

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FOXA2 and STAT5A regulate oncogenic activity of KIF5B-RET fusion.

American journal of cancer research

Lee MR,Shin JY,Kim MY,Kim JO,Jung CK,Kang J

Sat Mar 11 00:00:00 EST 2023

The long non-coding RNA T cell leukemia homeobox 1 neighbor enhances signal transducer and activator of transcription 5A phosphorylation to promote colon cancer cell invasion, migration, and metastasis.

Bioengineered

Chen G,Lian D,Zhao L,Wang Z,Wuyun Q,Zhang N

Fri Apr 01 00:00:00 EDT 2022

Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy.

Journal of agricultural and food chemistry

Shi X,Zhao L,Niu L,Yan Y,Chen Q,Jin Y,Li X

Wed Oct 19 00:00:00 EDT 2022

In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer.

Frontiers in oncology

Gong X,Liu X

Thu Dec 22 00:00:00 EST 2022

In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer.

Frontiers in oncology

Gong X,Liu X

Thu Dec 22 00:00:00 EST 2022

MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.

Chemosphere

Zhang Y,Hui J,Xu Y,Ma Y,Sun Z,Zhang M,Nie L,Ye L

Sun May 01 00:00:00 EDT 2022

MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.

Chemosphere

Zhang Y,Hui J,Xu Y,Ma Y,Sun Z,Zhang M,Nie L,Ye L

Sun May 01 00:00:00 EDT 2022

Protective effects of chlorogenic acid on inflammatory responses induced by Staphylococcus aureus and milk protein synthesis in bovine mammary epithelial cells.

Microbial pathogenesis

Ji Q,Zhang M,Wang Y,Chen Y,Wang L,Lu X,Bai L,Wang M,Bao L,Hao H,Wang Z

Sat Oct 01 00:00:00 EDT 2022

Intracellular Staphylococcus aureus Infection Decreases Milk Protein Synthesis by Preventing Amino Acid Uptake in Bovine Mammary Epithelial Cells.

Frontiers in veterinary science

Chen Y,Ma Y,Ji Q,Yang X,Feng X,Yao R,Cheng X,Li T,Wang Y,Wang Z

Tue Dec 07 00:00:00 EST 2021

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl₄/2-AAF.

Pharmaceutical biology

Xu Y,Xu W,Liu W,Chen G,Jiang S,Chen J,Jian X,Zhang H,Liu P,Mu Y

Wed Dec 01 00:00:00 EST 2021

C/EBPβ regulates the JAK/STAT signaling pathway in triple-negative breast cancer.

FEBS open bio

Wang S,Xia D,Wang X,Cao H,Wu C,Sun Z,Zhang D,Liu H

Thu Apr 01 00:00:00 EDT 2021

Effects of di (2-ethylhexyl) phthalate and high-fat diet on lipid metabolism in rats by JAK2/STAT5.

Environmental science and pollution research international

Zhang Y,Zhou L,Zhang Z,Xu Q,Han X,Zhao Y,Song X,Zhao T,Ye L

Sat Feb 01 00:00:00 EST 2020

IL-27 Rα⁺ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation.

Journal of cellular and molecular medicine

Zhao S,Liang T,Zhang C,Shi D,Jiang W,Su C,Hou G

Tue Sep 01 00:00:00 EDT 2020

LNK promotes the growth and metastasis of triple negative breast cancer via activating JAK/STAT3 and ERK1/2 pathway.

Cancer cell international

Lv J,Yu W,Zhang Y,Cao X,Han L,Hu H,Wang C

Thu Apr 14 00:00:00 EDT 2022

Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.

Ecotoxicology and environmental safety

Zhang Y,Wang S,Zhao T,Yang L,Guo S,Shi Y,Zhang X,Zhou L,Ye L

Sat Nov 30 00:00:00 EST 2019

Identification of miR-29c and its Target FBXO31 as a Key Regulatory Mechanism in Esophageal Cancer Chemoresistance: Functional Validation and Clinical Significance.

Theranostics

Li B,Hong P,Zheng CC,Dai W,Chen WY,Yang QS,Han L,Tsao SW,Chan KT,Lee NPY,Law S,Xu LY,Li EM,Chan KW,Qin YR,Guan XY,Lung ML,He QY,Xu WW,Cheung AL

Mon Mar 16 00:00:00 EDT 2020

Identification of miR-29c and its Target FBXO31 as a Key Regulatory Mechanism in Esophageal Cancer Chemoresistance: Functional Validation and Clinical Significance.

Theranostics

Li B,Hong P,Zheng CC,Dai W,Chen WY,Yang QS,Han L,Tsao SW,Chan KT,Lee NPY,Law S,Xu LY,Li EM,Chan KW,Qin YR,Guan XY,Lung ML,He QY,Xu WW,Cheung AL

Mon Mar 16 00:00:00 EDT 2020

IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation.

BMC pulmonary medicine

Dong Z,Lu X,Yang Y,Zhang T,Li Y,Chai Y,Lei W,Li C,Ai L,Tai W

Wed Feb 18 00:00:00 EST 2015

IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation.

BMC pulmonary medicine

Dong Z,Lu X,Yang Y,Zhang T,Li Y,Chai Y,Lei W,Li C,Ai L,Tai W

Wed Feb 18 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Mammary gland factor; mammary gland factor STAT5A; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; STAT; STAT5B

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Gene Aliases: AA959963; MGF; Mpf; STAT5; STAT5A

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UniProt ID: (Human) P42229, (Rat) Q62771, (Mouse) P42230

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Entrez Gene ID: (Human) 6776, (Rat) 24918, (Mouse) 20850

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding double-stranded DNA binding protein phosphatase binding sequence-specific DNA binding DNA-binding transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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STAT5A Polyclonal Antibody

STAT5A Antibody (13179-1-AP) in IHC (P)

Product Details

13179-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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