Invitrogen

SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), eBioscience™

FIGURE: 1 / 2

SSEA3 Antibody (14-8833-80) in Flow

Staining of the 2102Ep cell line with 0.5 µg of Rat IgM, K Isotype Control Purified (Product # 14-4341-82) (open histogram) or 0.5 µg of Anti-Human/Mouse SSEA-3 Purified (filled histogram) followed by Anti-Rat IgM PE (Product # 12-0990-82).Total viable cells were used for analysis.

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Product Details

14-8833-80

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rat / IgM

Class

Monoclonal

Type

Antibody

Clone

eBioMC-631 (MC-631)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657839

Product Specific Information

Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.

Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This eBioMC-631 (MC-631) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparison of the glycosphingolipids of human-induced pluripotent stem cells and human embryonic stem cells.

Glycobiology

Säljö K,Barone A,Vizlin-Hodzic D,Johansson BR,Breimer ME,Funa K,Teneberg S

14-8833 was used in Binding experiments to define the cell-surface carbohydrate antigen profile of human induced pluripotent stem cells relative to human embryonic stem cells.

Sat Apr 01 00:00:00 EDT 2017

ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Berger RP,Sun YH,Kulik M,Lee JK,Nairn AV,Moremen KW,Pierce M,Dalton S

Published figure using SSEA3 monoclonal antibody (Product # 14-8833-80) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Tian L,Prasad N,Jang YY

14-8833 was used in Flow cytometry/Cell sorting to study the effects of alcohol exposure on multistage hepatic cells to model the early stages of liver tissue injury associated with alcoholic liver disease.

Thu Aug 18 00:00:00 EDT 2016

Characterization of moose intestinal glycosphingolipids.

Glycoconjugate journal

Johansson MM,Dedic B,Lundholm K,Branzell FB,Barone A,Benktander J,Teneberg S

14-8833 was used in Affinity chromatography to isolate and identify glycolipids from three small intestines and one large intestine of the moose.

Sat Aug 01 00:00:00 EDT 2015

Sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells.

The Journal of biological chemistry

Barone A,Säljö K,Benktander J,Blomqvist M,Månsson JE,Johansson BR,Mölne J,Aspegren A,Björquist P,Breimer ME,Teneberg S

14-8833 was used in Flow cytometry/Cell sorting to characterise acid glycosphingolipids isolated from human embryonic stem cells, revealing a novel marker of undifferentiated cells, sialyl-lactotetra.

Fri Jul 04 00:00:00 EDT 2014

Structural complexity of non-acid glycosphingolipids in human embryonic stem cells grown under feeder-free conditions.

The Journal of biological chemistry

Barone A,Benktander J,Ångström J,Aspegren A,Björquist P,Teneberg S,Breimer ME

14-8833 was used in Binding experiments to show that the glycan diversity of human embryonic stem cells, including cell surface immune determinants, is more complex than previously appreciated.

Fri Apr 05 00:00:00 EDT 2013

Lectin-functionalized microchannels for characterizing pluripotent cells and early differentiation.

Biomicrofluidics

Vickers DA,Kulik M,Hincapie M,Hancock WS,Dalton S,Murthy SK

14-8833 was used in Flow cytometry/Cell sorting to track the differentiation of embryonic stem cells into the three embryonic germ layers; endoderm, mesoderm, and ectoderm, using surface lectins.

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: SSEA-3; stage-specific embryonic antigen 3

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