Invitrogen

SSEA3 Monoclonal Antibody (MC-631), DyLight™ 488

SSEA3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

SSEA3 Antibody (MA1-020-D488) in ICC/IF

Immunofluorescent analysis of SSEA-3 (green) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SSEA-3 monoclonal antibody (Product # MA1-020-D488) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834)... View More

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Product Details

MA1-020-D488

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

1:50-1:500

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgM

Class

Monoclonal

Type

Antibody

Clone

MC-631

Immunogen

Fischer rat immunized with 4-8 cell stage mouse embryos

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536683

Product Specific Information

MA1-020-D488 has been successfully used in ICC/IF and flow cytometry applications on human samples.

MA1-020-D488 can be used for immunofluoresence analysis of SSEA3 in human iPSCs.

Target Information

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: SSEA-3; stage-specific embryonic antigen 3

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