Invitrogen

SOX2 Monoclonal Antibody (10F10)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SOX2 Antibody in Immunocytochemistry (ICC/IF)

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SOX2 Antibody (MA5-15734) in ICC/IF

Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Mouse Monoclonal Antibody (Product # MA5-15734) at 1:250 dilution in 0.1% BSA,incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More

SOX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SOX2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis showed expression of SOX2 in NTERA-2 and not in HeLa which is reported as a negative model for SOX2 using SOX2 Monoclonal Antibody (Product # MA5-15734). {RE}

Product Details

MA5-15734

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

ELISA (ELISA)

1:10,000

ChIP assay (ChIP)

4 µl/10^6 cells

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10F10

Immunogen

Purified recombinant fragment of human SOX2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10980318

Product Specific Information

MA5-15734 targets SOX2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15734 immunogen is purified recombinant fragment of human SOX2 expressed in E. Coli.

MA5-15734 detects SOX2 which has a predicted molecular weight of approximately 34kDa.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

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Gene Aliases: ANOP3; MCOPS3; SOX2

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UniProt ID: (Human) P48431

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Entrez Gene ID: (Human) 6657

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Function(s)

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding HMG box transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation eye development endodermal cell fate specification chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell cycle arrest response to wounding regulation of gene expression glial cell fate commitment pituitary gland development adenohypophysis development positive regulation of cell-cell adhesion forebrain development somatic stem cell population maintenance tissue regeneration regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter inner ear development negative regulation of epithelial cell proliferation response to growth factor negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance

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SOX2 Monoclonal Antibody (10F10)

SOX2 Antibody (MA5-15734) in ICC/IF

Product Details

MA5-15734

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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