Invitrogen

SOD1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

12 References

View all (78) SOD1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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SOD1 Antibody (PA5-27240) in WB

Western blot analysis was performed on whole cell extracts of HEK-293 (Lane 1), HEK-293 treated with H2O2 (10uM, 6hrs) (Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), and SH-SY5Y (Lane 5). The blot was probed with SOD1 Polyclonal Antibody (Product # PA5-27240, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 18kDa band corresponding to SOD1 was observed across the panel and was upregulated upon H2O2 treatmen... View More

SOD1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

SOD1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of SOD1 using SOD1 Polyclonal Antibody (Product # PA5-27240) shows an increase in expression upon treatment with H2O2 in HEK-293 cells. {TM}

Product Details

PA5-27240

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 75 and 154 of Human SOD1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544716

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, NT2D1, PC-3, SK-N-SH, Rat brain, U87-MG.

Predicted reactivity: Mouse (100%), Rat (100%), Chimpanzee (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

Published figure using SOD1 polyclonal antibody (Product # PA5-27240) in Western Blot

Sat Aug 19 00:00:00 EDT 2023

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

Published figure using SOD1 polyclonal antibody (Product # PA5-27240) in Western Blot

Sat Aug 19 00:00:00 EDT 2023

Chronic oxidative stress adaptation in head and neck cancer cells generates slow-cyclers with decreased tumour growth in vivo.

British journal of cancer

Berner J,Miebach L,Kordt M,Seebauer C,Schmidt A,Lalk M,Vollmar B,Metelmann HR,Bekeschus S

PA5-27240 was used in Simple Western - Size to show that combination treatments together with cetuximab, an EGFR inhibitor, may overcome acquired oxidative stress resistance in HNC.

Fri Sep 01 00:00:00 EDT 2023

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

PA5-27240 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 EDT 2022

Expression of antioxidant enzymes in lesions of multiple sclerosis and its models.

Scientific reports

Moezzi D,Dong Y,Jain RW,Lozinski BM,Ghorbani S,D'Mello C,Wee Yong V

PA5-27240 was used in Immunohistochemistry-immunofluorescence to investigate whether antioxidant enzyme elevation in mouse models of neurodegeneration corresponds to their expression in human diseases such as multiple sclerosis.

Tue Jul 26 00:00:00 EDT 2022

Expression of antioxidant enzymes in lesions of multiple sclerosis and its models.

Scientific reports

Moezzi D,Dong Y,Jain RW,Lozinski BM,Ghorbani S,D'Mello C,Wee Yong V

Tue Jul 26 00:00:00 EDT 2022

Hatched Eggshell Membrane Can Be a Novel Source of Antioxidant Hydrolysates to Protect against H₂O₂-Induced Oxidative Stress in Human Chondrocytes.

Antioxidants (Basel, Switzerland)

Zhu L,Ma M,Ahn DU,Guyonnet V,Wang L,Zheng Y,He Q,Xiong H,Huang X

PA5-27240 was used in Immunohistochemistry (Frozen), Western Blot to provide a theoretical basis for the value-added application of hatched ESM waste to produce antioxidant hydrolysates and indicates their potential as functional food and pharmaceuticals.

Fri Dec 09 00:00:00 EST 2022

The Transcriptional Complex Sp1/KMT2A by Up-Regulating Restrictive Element 1 Silencing Transcription Factor Accelerates Methylmercury-Induced Cell Death in Motor Neuron-Like NSC34 Cells Overexpressing SOD1-G93A.

Frontiers in neuroscience

Guida N,Sanguigno L,Mascolo L,Calabrese L,Serani A,Molinaro P,Lau CG,Annunziato L,Formisano L

PA5-27240 was used in ChIP assay to demonstrate that MeHg hastens necroptotic cell death in SOD1-G93A cells via Sp1/KMT2A complex, that by epigenetic mechanisms increases REST gene expression.

Wed Dec 15 00:00:00 EST 2021

Frontiers in neuroscience

Guida N,Sanguigno L,Mascolo L,Calabrese L,Serani A,Molinaro P,Lau CG,Annunziato L,Formisano L

PA5-27240 was used in ChIP assay to demonstrate that MeHg hastens necroptotic cell death in SOD1-G93A cells via Sp1/KMT2A complex, that by epigenetic mechanisms increases REST gene expression.

Wed Dec 15 00:00:00 EST 2021

Resveratrol treatment reduces the vulnerability of SH-SY5Y cells and cortical neurons overexpressing SOD1-G93A to Thimerosal toxicity through SIRT1/DREAM/PDYN pathway.

Neurotoxicology

Laudati G,Mascolo L,Guida N,Sirabella R,Pizzorusso V,Bruzzaniti S,Serani A,Di Renzo G,Canzoniero LMT,Formisano L

PA5-27240 was used in Western Blot to demonstrate the particular vulnerability of SOD1-G93 A neuronal cells to thimerosal and that RSV via SIRT1 counteracts the neurodetrimental effect of this toxicant by preventing DREAM reduction and prodynorphin up-regulation.

Fri Mar 01 00:00:00 EST 2019

Oxidative stress and mitochondrial uncoupling protein 2 expression in hepatic steatosis induced by exposure to xenobiotic DDE and high fat diet in male Wistar rats.

PloS one

Migliaccio V,Scudiero R,Sica R,Lionetti L,Putti R

PA5-27240 was used in Western Blotting to aimed to evaluate hepatic oxidative stress and anti-oxidant systems response in rats exposed to HFD and/or non-toxic dose of dichlorodiphenyldichloroethylene.

Wed Jan 08 00:00:00 EST 2020

Methylmercury alters proliferation, migration, and antioxidant capacity in human HTR8/SV-neo trophoblast cells.

Reproductive toxicology (Elmsford, N.Y.)

Tucker EK,Nowak RA

Published figure using SOD1 polyclonal antibody (Product # PA5-27240) in Western Blot

Fri Jun 01 00:00:00 EDT 2018

In vivo genome editing improves motor function and extends survival in a mouse model of ALS.

Science advances

Gaj T,Ojala DS,Ekman FK,Byrne LC,Limsirichai P,Schaffer DV

PA5-27240 was used in Western Blotting to demonstrate that CRISPR-Cas9 can be harnessed to disrupt mutant SOD1 expression in the G93ASOD1 mouse model of Amyotrophic lateral sclerosis (ALS) following in vivo delivery using an adeno-associated virus vector.

Fri Dec 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic

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Gene Aliases: ALS; ALS1; B430204E11Rik; Cu/Zn-SOD; CuZnSOD; HEL-S-44; homodimer; hSod1; Ipo-1; Ipo1; IPOA; SOD; Sod-1; SOD1; SODC

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UniProt ID: (Human) P00441, (Mouse) P08228, (Rat) P07632

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Entrez Gene ID: (Human) 6647, (Mouse) 20655, (Rat) 24786

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Function(s)

superoxide dismutase activity copper ion binding protein binding zinc ion binding protein phosphatase 2B binding identical protein binding protein homodimerization activity Rac GTPase binding chaperone binding antioxidant activity oxidoreductase activity enzyme binding metal ion binding oxidoreductase

Process(es)

activation of MAPK activity response to reactive oxygen species response to superoxide ovarian follicle development positive regulation of cytokine production placenta development retina homeostasis response to amphetamine myeloid cell homeostasis platelet degranulation glutathione metabolic process superoxide metabolic process cellular iron ion homeostasis spermatogenesis embryo implantation cell aging sensory perception of sound locomotory behavior anterograde axonal transport retrograde axonal transport regulation of blood pressure response to heat response to organic substance transmission of nerve impulse removal of superoxide radicals response to nutrient levels peripheral nervous system myelin maintenance positive regulation of superoxide anion generation regulation of T cell differentiation in thymus response to carbon monoxide cellular response to potassium ion regulation of multicellular organism growth response to drug response to hydrogen peroxide superoxide anion generation positive regulation of apoptotic process positive regulation of catalytic activity regulation of GTPase activity negative regulation of neuron apoptotic process response to ethanol negative regulation of cholesterol biosynthetic process regulation of protein kinase activity regulation of organ growth response to antibiotic response to copper ion muscle cell cellular homeostasis thymus development response to axon injury hydrogen peroxide biosynthetic process regulation of mitochondrial membrane potential oxidation-reduction process heart contraction neurofilament cytoskeleton organization relaxation of vascular smooth muscle auditory receptor cell stereocilium organization cellular response to cadmium ion cellular response to ATP reactive oxygen species metabolic process response to antipsychotic drug positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway response to oxidative stress aging negative regulation of apoptotic process double-strand break repair apoptotic DNA fragmentation cellular response to oxidative stress

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SOD1 Polyclonal Antibody

SOD1 Antibody (PA5-27240) in WB

Product Details

PA5-27240

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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