Invitrogen

Red Firefly Luciferase Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (23) Firefly Luciferase antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Red Firefly Luciferase Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

Red Firefly Luciferase Antibody (PA1-179) in ICC/IF

Immunofluorescent analysis of Red firefly luciferase (from the Japanese firefly Luciola cruciata) in pCMV-Red Firefly (RFF) Luc transfected (green, left panel) and untransfected (UT) 293 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cel... View More

Red Firefly Luciferase Antibody in Western Blot (WB)

Red Firefly Luciferase Antibody in Immunoprecipitation (IP)

Red Firefly Luciferase Antibody in Neutralization (Neu)

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Product Details

PA1-179

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:4,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:500-1:1,000

Immunoprecipitation (IP)

5 µg

Neutralization (Neu)

Assay-dependent

Product Specifications

Species Reactivity

Luciferase

Published species

Luciferase

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified full length recombinant Red Firefly luciferase expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539910

Product Specific Information

Western blot analysis of PA1-179 detects a prominent ~62 kDa protein in Red Firefly luciferase expressing cells. No signal was detected in Green Renilla transfected or untransfected control HEK293 cells. This antibody has also been successfully tested in IP, Immunofluorescence and neutralization assays.

Red Firefly luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash
or Glow Assay Kits or Luciferase Dual Assay Kits.

Target Information

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Knockout of the caspase 8-associated protein 2 gene improves recombinant protein expression in HEK293 cells through up-regulation of the cyclin-dependent kinase inhibitor 2A gene.

Biotechnology and bioengineering

Abaandou L,Sharma AK,Shiloach J

Published figure using Red Firefly Luciferase polyclonal antibody (Product # PA1-179) in Western Blot

Fri Jan 01 00:00:00 EST 2021

Knocking out Ornithine Decarboxylase Antizyme 1 (OAZ1) Improves Recombinant Protein Expression in the HEK293 Cell Line.

Medical sciences (Basel, Switzerland)

Abaandou L,Shiloach J

PA1-179 was used in Western Blotting to indicate that genetic inactivation of ornithine decarboxylase antizyme 1 in human embryonic kidney 293 cells can improve recombinant protein expression.

Fri Jun 08 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; luciferin 4-monooxygenase; Photinus pyralis; photinus pyralis luciferase

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