Invitrogen

RXRA Monoclonal Antibody (K8508)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 References
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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

433900

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
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Immunohistochemistry (Paraffin) (IHC (P))

3-20 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

ELISA (ELISA)

0.1 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
-

ChIP assay (ChIP)

Assay-dependent
View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

K8508

Immunogen

Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant human RXR--alpha (2-133 aa).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

physiological saline

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Wet ice

RRID

AB_2532208

Product Specific Information

This antibody specifically recognizes human RXR alpha and cross reacts with mouse and rat RXR alpha. This antibody does not recognize human RXR beta and gamma.

Target Information

Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 2 group B member 1; Retinoic acid receptor RXR-alpha; retinoid X nuclear receptor alpha; retinoid X receptor; Retinoid X receptor alpha; retinoid X receptor, alpha; rxr alpha; RXR alpha 1

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Gene Aliases: 9530071D11Rik; NR2B1; RXRA; RXRalpha1

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UniProt ID: (Human) P19793, (Mouse) P28700

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Entrez Gene ID: (Human) 6256, (Rat) 25271, (Mouse) 20181

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity retinoic acid receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding 9-cis retinoic acid receptor activity protein binding zinc ion binding enzyme binding protein domain specific binding chromatin DNA binding protein complex binding vitamin D receptor binding retinoic acid receptor binding sequence-specific DNA binding transcription regulatory region DNA binding retinoic acid-responsive element binding protein heterodimerization activity vitamin D response element binding double-stranded DNA binding transcription factor binding metal ion binding C4 zinc finger nuclear receptor
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development liver development maternal placenta development transcription initiation from RNA polymerase II promoter vitamin metabolic process inflammatory response midgut development embryo implantation aging cholesterol metabolic process negative regulation of cell proliferation response to selenium ion bile acid and bile salt transport modulation by virus of host morphology or physiology axon regeneration regulation of myelination response to retinoic acid cellular response to insulin stimulus response to vitamin A response to vitamin D peroxisome proliferator activated receptor signaling pathway camera-type eye development positive regulation of apoptotic process steroid hormone mediated signaling pathway cellular lipid metabolic process response to ethanol positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron retinoic acid receptor signaling pathway protein homotetramerization response to glucocorticoid ventricular cardiac muscle tissue morphogenesis ventricular cardiac muscle cell differentiation cardiac muscle cell proliferation secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development regulation of branching involved in prostate gland morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus placenta development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter heart development female pregnancy response to nutrient response to organic cyclic compound positive regulation of transcription, DNA-templated heart morphogenesis negative regulation of gene expression cardiac muscle cell differentiation mesenchyme development angiogenesis involved in coronary vascular morphogenesis visceral serous pericardium development
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