Invitrogen

RICTOR Monoclonal Antibody (7B3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 References

View all (25) RICTOR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

RICTOR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

RICTOR Antibody (MA5-15681) in ICC/IF

Immunofluorescent analysis of Rictor (magenta) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a Rictor monoclonal antibody (Product # MA5-15681) at a dilution of 1:200 in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-mouse IgG Secondary Antibody, Alexa Fluor 647 conjugate (Product # A32728) at a dilution of 1:1000 for 1 hou... View More

RICTOR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RICTOR Antibody in Flow Cytometry (Flow)

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Product Details

MA5-15681

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7B3

Immunogen

Purified recombinant fragment of human RICTOR expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10987411

Product Specific Information

MA5-15681 targets RICTOR in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.

The MA5-15681 immunogen is purified recombinant fragment of human RICTOR expressed in E. Coli.

MA5-15681 detects RICTOR which has a predicted molecular weight of approximately 192kDa.

Target Information

RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.

Cancers

Moritz S,Krause M,Schlatter J,Cordes N,Vehlow A

MA5-15681 was used in Proximity Ligation Assay (PLA) to show that the cytoskeleton regulator Lamellipodin mediates invasiveness, proliferation and radiosensitivity of glioblastoma cells.

Sun Oct 24 00:00:00 EDT 2021

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.

Nature communications

Mathieu J,Detraux D,Kuppers D,Wang Y,Cavanaugh C,Sidhu S,Levy S,Robitaille AM,Ferreccio A,Bottorff T,McAlister A,Somasundaram L,Artoni F,Battle S,Hawkins RD,Moon RT,Ware CB,Paddison PJ,Ruohola-Baker H

MA5-15681 was used in Western Blotting to demonstrate the mechanisms behind how cells exit human pluripotency by CRISPR-Cas9.

Thu Feb 07 00:00:00 EST 2019

Bioinformatics

Protein Aliases: AVO3 homolog; DKFZp686B11164; hAVO3; mAVO3; MGC39830; pianissimo; Protein pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3

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Gene Aliases: 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik; hAVO3; KIAA1999; PIA; RICTOR

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UniProt ID: (Human) Q6R327, (Mouse) Q6QI06

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Entrez Gene ID: (Human) 253260, (Mouse) 78757

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Function(s)

protein binding enzyme activator activity protein kinase binding ribosome binding

Process(es)

positive regulation of endothelial cell proliferation multicellular organism development embryo development regulation of gene expression peptidyl-serine phosphorylation positive regulation of actin filament polymerization actin cytoskeleton reorganization TOR signaling positive regulation of TOR signaling regulation of actin cytoskeleton organization regulation of peptidyl-serine phosphorylation regulation of phosphorylation regulation of GTPase activity regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation regulation of protein kinase B signaling positive regulation of protein kinase B signaling regulation of establishment of cell polarity establishment of cell polarity establishment or maintenance of actin cytoskeleton polarity T cell costimulation positive regulation of catalytic activity phosphatidylinositol-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RICTOR Monoclonal Antibody (7B3)

RICTOR Antibody (MA5-15681) in ICC/IF

Product Details

MA5-15681

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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