Invitrogen

RAD51 Monoclonal Antibody (14B4)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (42) RAD51 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

RAD51 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

RAD51 Antibody (MA1-23271) in ICC/IF

RAD51 Monoclonal Antibody (14B4) detects Rad51 protein at nuclear foci by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad51 stained by RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) diluted at 1:500. Blue: Fluoroshield with DAPI .

RAD51 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. 293T cells were transfected with RAD51 siRNA and decrease in signal intensity was observed in western blot application using RAD51 Antibody (Product # MA1-23271). {KD}

Product Details

MA1-23271

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

in situ PLA (PLA)

Assay-dependent

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14B4

Immunogen

Full length Rad51 protein (amino acids 1-338) expressed in E. coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560832

Product Specific Information

A suggested positive control for this product is T24.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage.

Cell death & disease

Steffens Reinhardt L,Zhang X,Groen K,Morten BC,De Iuliis GN,Braithwaite AW,Bourdon JC,Avery-Kiejda KA

MA1-23271 was used in Immunocytochemistry-immunofluorescence to suggest that the response of breast cancer cells to standard of care DNA-damaging therapies is dependent on the expression of p53 isoforms, which may contribute to outcomes in breast cancer.

Fri Oct 28 00:00:00 EDT 2022

A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage.

Genes & development

van Sluis M,McStay B

MA1-23271 was used in immunocytochemistry to study the response to double-stranded breaks at the nucleolar organizer regions.

Mon Jun 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HsRAD51; RAD51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

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Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603

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UniProt ID: (Human) Q06609, (Chicken) P37383, (Mouse) Q08297

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Entrez Gene ID: (Human) 5888, (Chicken) 396086, (Mouse) 19361, (Rat) 499870

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Function(s)

recombinase activity four-way junction DNA binding chromatin binding double-stranded DNA binding single-stranded DNA binding endodeoxyribonuclease activity protein binding ATP binding protein C-terminus binding identical protein binding single-stranded DNA-dependent ATPase activity DNA polymerase binding DNA-dependent ATPase activity single-stranded DNA-dependent ATP-dependent DNA helicase activity nucleotide binding DNA binding damaged DNA binding molecular_function DNA metabolism protein

Process(es)

telomere maintenance via recombination double-strand break repair via homologous recombination DNA recombinase assembly DNA synthesis involved in DNA repair strand displacement regulation of protein phosphorylation DNA unwinding involved in DNA replication DNA repair DNA recombination mitotic recombination cellular response to DNA damage stimulus meiotic nuclear division reciprocal meiotic recombination response to toxic substance response to X-ray regulation of double-strand break repair via homologous recombination telomere maintenance via telomere lengthening replication fork processing strand invasion response to drug positive regulation of DNA ligation protein homooligomerization chromosome organization involved in meiotic cell cycle cellular response to ionizing radiation cellular response to gamma radiation cellular response to hydroxyurea cellular response to cisplatin cellular response to camptothecin response to etoposide replication-born double-strand break repair via sister chromatid exchange mitotic recombination-dependent replication fork processing response to ionizing radiation interstrand cross-link repair meiotic cell cycle DNA metabolic process telomere organization cellular response to alkaloid

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RAD51 Monoclonal Antibody (14B4)

RAD51 Antibody (MA1-23271) in ICC/IF

Product Details

MA1-23271

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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