Invitrogen

RAD51 Polyclonal Antibody

View all (42) RAD51 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-86645

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, corresponding to amino acids 280-330 of Human Rad51.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2803411

Product Specific Information

Purity is > 95% by SDS-PAGE.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

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Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA

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UniProt ID: (Human) Q06609, (Mouse) Q08297

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Entrez Gene ID: (Human) 5888, (Mouse) 19361

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Function(s)
recombinase activity four-way junction DNA binding chromatin binding double-stranded DNA binding single-stranded DNA binding endodeoxyribonuclease activity protein binding ATP binding protein C-terminus binding identical protein binding single-stranded DNA-dependent ATPase activity DNA polymerase binding nucleotide binding DNA binding damaged DNA binding DNA-dependent ATPase activity DNA metabolism protein
Process(es)
telomere maintenance via recombination double-strand break repair via homologous recombination DNA recombinase assembly DNA synthesis involved in DNA repair strand displacement regulation of protein phosphorylation DNA unwinding involved in DNA replication DNA repair DNA recombination mitotic recombination cellular response to DNA damage stimulus meiotic nuclear division reciprocal meiotic recombination response to toxic substance response to X-ray regulation of double-strand break repair via homologous recombination telomere maintenance via telomere lengthening replication fork processing strand invasion response to drug positive regulation of DNA ligation protein homooligomerization chromosome organization involved in meiotic cell cycle cellular response to ionizing radiation cellular response to gamma radiation cellular response to hydroxyurea cellular response to cisplatin cellular response to camptothecin response to etoposide replication-born double-strand break repair via sister chromatid exchange mitotic recombination-dependent replication fork processing DNA metabolic process telomere organization cellular response to alkaloid
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