Invitrogen

RAB5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

5 References

View all (22) RAB5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

RAB5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

RAB5 Antibody (PA5-29022) in ICC/IF

MRC-5 cells (fetal lung fibroblasts) were grown on glass coverslips in 6 well dishes and infected for 48 h with VZV. Cells were fixed in 2% Paraformaldehyde and permeabilized with 0.02% Triton X-100 for 1 h at room temperature. Blocked cells for 2 h at RT in 5% non-fat milk plus 2.5% normal goat serum in PBS. Anti-VZV gE mouse monoclonal antibody (red) and anti-Rab5 rabbit polyclonal antibody (green, Product # PA5-29022, 1:100 in PBS) were incubated 2 h at RT (with rocking), then overnight at 4oC (with gentle rocking). After washing with P... View More

RAB5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB5 Antibody in Immunoprecipitation (IP)

Product Image Gallery

RAB5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with RAB5 siRNA and reduction of signal was observed in Western Blot using RAB5 Polyclonal Antibody (Product # PA5-29022). [Note: Non-specific band observed at ~50Kda]. {KD}

Product Details

PA5-29022

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 132 and 215 of Human RAB5A

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546498

Product Specific Information

Recommended positive controls: A431, Raji, mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RAB5A expression is a predictive biomarker for trastuzumab emtansine in breast cancer.

Nature communications

Engebraaten O,Yau C,Berg K,Borgen E,Garred Ø,Berstad MEB,Fremstedal ASV,DeMichele A,Veer LV',Esserman L,Weyergang A

Published figure using RAB5 polyclonal antibody (Product # PA5-29022) in Western Blot

Fri Nov 05 00:00:00 EDT 2021

Characterization of Small-Molecule-Induced Changes in Parkinson's-Related Trafficking via the Nedd4 Ubiquitin Signaling Cascade.

Cell chemical biology

Hatstat AK,Ahrendt HD,Foster MW,Mayne L,Moseley MA,Englander SW,McCafferty DG

PA5-29022 was used in Immunocytochemistry-immunofluorescence to investigate the effect of NAB2 on Nedd4 activity and specificity.

Thu Jan 21 00:00:00 EST 2021

Cutaneous T-Cell Lymphoma (CTCL) Cell Line-Derived Extracellular Vesicles Contain HERV-W-Encoded Fusogenic Syncytin-1.

The Journal of investigative dermatology

Laukkanen K,Saarinen M,Mallet F,Aatonen M,Hau A,Ranki A

PA5-29022 was used in Western Blotting to examine the expression and functional properties of syncytin-1 in verified cell lines of the aforementioned forms of CTCLs and also its presence in the extracellular vesicles (EVs) released by the malignant cells.

Wed Jul 01 00:00:00 EDT 2020

Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles.

Cancers

Palviainen M,Laukkanen K,Tavukcuoglu Z,Velagapudi V,Kärkkäinen O,Hanhineva K,Auriola S,Ranki A,Siljander P

Published figure using RAB5 polyclonal antibody (Product # PA5-29022) in Western Blot

Fri Nov 06 00:00:00 EST 2020

Trappc9 deficiency in mice impairs learning and memory by causing imbalance of dopamine D1 and D2 neurons.

Science advances

Ke Y,Weng M,Chhetri G,Usman M,Li Y,Yu Q,Ding Y,Wang Z,Wang X,Sultana P,DiFiglia M,Li X

PA5-29022 was used in Western Blot to report that trappc9-deficient mice exhibit a broad range of behavioral deficits and postnatal delay in growth of the brain.

Sun Nov 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Rab 5; Rab 5A; Ras related protein; RAS-associated protein RAB5A; Ras-related protein Rab-5A; Small GTP-binding protein rab5

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Gene Aliases: 2410015H04Rik; AI663973; AU021172; nnyRab5a; RAB5; RAB5A

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UniProt ID: (Human) P20339, (Mouse) Q9CQD1, (Rat) M0RC99

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Entrez Gene ID: (Human) 5868, (Mouse) 271457, (Rat) 64633

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Function(s)

GTPase activity protein binding GTP binding GDP binding nucleotide binding guanyl nucleotide binding GDP-dissociation inhibitor binding

Process(es)

endocytosis phagocytosis small GTPase mediated signal transduction blood coagulation protein transport regulation of endocytosis synaptic vesicle recycling early endosome to late endosome transport positive regulation of exocytosis regulation of endosome size regulation of filopodium assembly receptor internalization involved in canonical Wnt signaling pathway regulation of synaptic vesicle exocytosis regulation of autophagosome assembly transport intracellular protein transport endosome organization positive regulation of smooth muscle cell migration Rab protein signal transduction regulation of long-term neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation vesicle-mediated transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAB5 Polyclonal Antibody

RAB5 Antibody (PA5-29022) in ICC/IF

Product Details

PA5-29022

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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