Invitrogen

Phospho-p70 S6 Kinase (Thr389) Monoclonal Antibody (R.566.2)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Reference
View all (87) p70 S6 Kinase antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15202

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication
Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

R.566.2

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr389 of human p70 S6 kinase
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

719 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10978757

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; CG10539-PA; CG10539-PB; Dp70s6k; dS6 kinase; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase; p70 ribosomal S6 kinase alpha; p70 S6 kinase; p70 S6 kinase 1; p70 S6 kinase alpha; p70 S6 kinase, alpha; p70 S6K-alpha; p70 S6KA; p70(S6K)-alpha; p70-S6K 1; p70S6 kinase; P70S6K; p70S6K kinase; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; protein kinase-like 64F; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; ribosomal S6 protein kinase; RPS6-p70-protein kinase; S6 kinase; S6-kinase; S6K; S6K kinase; S6K-beta-1; S6k-PA; S6k-PB; S6K1; S6kinase; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A

View more View less

Gene Aliases: 10539; 2610318I15Rik; 4732464A07Rik; 7-10; 7084; 70kDa; AA959758; AI256796; AI314060; CG10539; d-S6K; Dmel\CG10539; Dmel_CG10539; Dp70[s6k]; Dp70S6k; dps6k; DS6K; dS6K1; fs(3)07084; l(3)07084; p-S6K; p70 S6K; p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; p70/85s6k; p70/S6K; p70[S6 kinase]; p70[S6K]; p70[S6kinase]; p70S6K; Pk64F; Pk?6; PS6K; RPS6KB1; S6 K; S6K; S6K-beta-1; S6K1; s6k11; S6k|S6K; STK14A

View more View less

UniProt ID: (Human) P23443, (Mouse) Q8BSK8, (Rat) P67999

View more View less

Entrez Gene ID: (Human) 6198, (Mouse) 72508, (Rat) 83840, (Fruit fly) 38654

View more View less

Function(s)
protein kinase activity ribosomal protein S6 kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity PDZ domain binding peptide binding identical protein binding protein phosphatase 2A binding nucleotide binding protein serine/threonine kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein modifying enzyme
Process(es)
G1/S transition of mitotic cell cycle behavioral fear response skeletal muscle contraction protein phosphorylation apoptotic process signal transduction germ cell development aging response to nutrient long-term memory response to heat response to wounding response to mechanical stimulus response to toxic substance response to glucose skeletal muscle atrophy response to electrical stimulus involved in regulation of muscle adaptation positive regulation of smooth muscle cell migration cell migration TOR signaling response to lipopolysaccharide response to insulin cellular response to hormone stimulus response to testosterone response to glucagon response to tumor necrosis factor response to drug negative regulation of apoptotic process response to leucine protein kinase B signaling response to ethanol positive regulation of translation positive regulation of mitotic cell cycle positive regulation of translational initiation regulation of glucose import negative regulation of insulin receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of skeletal muscle tissue growth positive regulation of smooth muscle cell proliferation response to glucocorticoid cellular response to growth factor stimulus cellular response to organic cyclic compound negative regulation of extrinsic apoptotic signaling pathway regulation of translation cell cycle phosphorylation cellular response to insulin stimulus response to organic substance response to organonitrogen compound response to organic cyclic compound response to amino acid response to peptide hormone response to oxidative stress insulin receptor signaling pathway determination of adult lifespan positive regulation of autophagy positive regulation of cell growth larval feeding behavior multicellular organism growth intracellular signal transduction female germ-line stem cell population maintenance TORC1 signaling positive regulation of cell size vascular endothelial growth factor receptor signaling pathway dendrite morphogenesis synaptic growth at neuromuscular junction lipid homeostasis cellular response to amino acid stimulus positive regulation of axon guidance regulation of terminal button organization regulation of response to drug
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support