Invitrogen

Phospho-SMAD1 (Ser187) Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-105003

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
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Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMAD1(Accession Q15797), corresponding to amino acid residues around phosphorylated Ser187.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816476

Product Specific Information

Antibody detects endogenous levels of Smad1 only when phosphorylated at Serine 187.

Target Information

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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Gene Aliases: AI528653; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mlp1; MusMLP; SMAD1

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UniProt ID: (Human) Q15797, (Mouse) P70340

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Entrez Gene ID: (Human) 4086, (Mouse) 17125

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding protein kinase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding DNA binding DNA-binding transcription factor gene-specific transcriptional regulator
Process(es)
MAPK cascade ureteric bud development mesodermal cell fate commitment osteoblast fate commitment transcription from RNA polymerase II promoter inflammatory response signal transduction transforming growth factor beta receptor signaling pathway SMAD protein complex assembly gamete generation negative regulation of cell proliferation embryonic pattern specification positive regulation of gene expression BMP signaling pathway midbrain development hindbrain development primary miRNA processing homeostatic process positive regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter cartilage development cardiac muscle cell proliferation bone development SMAD protein signal transduction positive regulation of cartilage development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation response to drug positive regulation of cell differentiation cellular response to organic cyclic compound cellular response to BMP stimulus
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