Invitrogen

PRMT5 Recombinant Rabbit Monoclonal Antibody (2H25L22)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PRMT5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

PRMT5 Antibody (703704) in ICC/IF

For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized using ice cold 100% Acetone for detection of endogenous PRMT5 using Anti-PRMT5 Recombinant Rabbit Monoclonal Antibody (Product # 703704, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of PRMT5 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifa... View More

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PRMT5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. MCF7 cells were transfected with PRMT5 siRNA and a decrease in signal intensity was observed in Western blot application using Anti-PRMT5 Recombinant Rabbit Monoclonal Antibody (Product # 703704). {KD}

Product Details

703704

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

2H25L22

Immunogen

Protein corresponding to human PRMT5 [aa1-aa291]

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2784600

Product Specific Information

This antibody is predicted to react with Monkey, Cat, Dog.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1 homolog

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Gene Aliases: HRMT1L5; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

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UniProt ID: (Human) O14744

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Entrez Gene ID: (Human) 10419

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Function(s)

core promoter sequence-specific DNA binding transcription corepressor activity protein binding methyltransferase activity methyl-CpG binding histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity protein complex binding protein-arginine omega-N symmetric methyltransferase activity ribonucleoprotein complex binding histone methyltransferase activity (H4-R3 specific) protein heterodimerization activity protein modifying enzyme

Process(es)

spliceosomal snRNP assembly DNA-templated transcription, termination regulation of transcription, DNA-templated regulation of mitotic nuclear division cell proliferation peptidyl-arginine methylation peptidyl-arginine methylation, to symmetrical-dimethyl arginine circadian regulation of gene expression peptidyl-arginine N-methylation endothelial cell activation histone H4-R3 methylation regulation of DNA methylation negative regulation of cell differentiation positive regulation of oligodendrocyte differentiation regulation of ERK1 and ERK2 cascade Golgi ribbon formation liver regeneration regulation of signal transduction by p53 class mediator negative regulation of nucleic acid-templated transcription positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRMT5 Recombinant Rabbit Monoclonal Antibody (2H25L22)

PRMT5 Antibody (703704) in ICC/IF

Product Details

703704

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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