Active Motif

PRMT5 Polyclonal Antibody

FIGURE: 1 / 1

PRMT5 Antibody (61001) in WB

Western blot of PRMT5 antibody. Cytoplasmic extract of Jurkat cells (30 µg per lane) probed with PRMT5 antibody (1:1,000).

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Product Details

61001

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

ChIP-sequencing (ChIP-Seq)

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This PRMT5 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human PRMT5.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

70mM tris, pH 8, with 30% glycerol, 0.07mM EDTA, 105mM NaCl, 31mM glycine

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2615010

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KSHV encoded ORF59 modulates histone arginine methylation of the viral genome to promote viral reactivation.

PLoS pathogens

Strahan RC,McDowell-Sargent M,Uppal T,Purushothaman P,Verma SC

Research topic: Virology - KSHV

Sat Jul 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1 homolog

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Gene Aliases: HRMT1L5; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

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UniProt ID: (Human) O14744

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Entrez Gene ID: (Human) 10419

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Function(s)

core promoter sequence-specific DNA binding transcription corepressor activity protein binding methyltransferase activity methyl-CpG binding histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity protein complex binding protein-arginine omega-N symmetric methyltransferase activity ribonucleoprotein complex binding histone methyltransferase activity (H4-R3 specific) protein heterodimerization activity protein modifying enzyme

Process(es)

spliceosomal snRNP assembly DNA-templated transcription, termination regulation of transcription, DNA-templated regulation of mitotic nuclear division cell proliferation peptidyl-arginine methylation peptidyl-arginine methylation, to symmetrical-dimethyl arginine circadian regulation of gene expression peptidyl-arginine N-methylation endothelial cell activation histone H4-R3 methylation regulation of DNA methylation negative regulation of cell differentiation positive regulation of oligodendrocyte differentiation regulation of ERK1 and ERK2 cascade Golgi ribbon formation liver regeneration regulation of signal transduction by p53 class mediator negative regulation of nucleic acid-templated transcription positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRMT5 Polyclonal Antibody

PRMT5 Antibody (61001) in WB

Product Details

61001

Western Blot (WB)

ChIP-sequencing (ChIP-Seq)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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