Invitrogen

PRMT5 Recombinant Mouse Monoclonal Antibody

PRMT5 Antibody in Immunocytochemistry (ICC/IF)

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PRMT5 Antibody (730054) in ICC/IF

Immunofluorescence analysis of PRMT5 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRMT5 Mouse Monoclonal Antibody (Product # 730054) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilutio... View More

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunoprecipitation (IP)

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Product Details

730054

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Recombinant Monoclonal

Type

Antibody

Immunogen

269-637 of 637 of PRMT5 Protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Wet ice

RRID

AB_2532855

Product Specific Information

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.

Nature methods

Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gräslund S,Greenblatt JF,Edwards AM

730054 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality

Sat Aug 01 00:00:00 EDT 2015

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