Invitrogen

PDGFRA Recombinant Rabbit Monoclonal Antibody (7H13L1)

2 References
View all (78) PDGFRA (CD140a) antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

701142

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-3 µg/mL
View 1 publication 1 publication

ChIP assay (ChIP)

1 µL
-
Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

7H13L1

Immunogen

Recombinant protein corresponding to amino acids 259-436 of human platelet-derived growth factor receptor - alpha polypeptide
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532408

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; CD140a antigen; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF Receptor alpha; PDGF-R-alpha; PDGFR-2; pdgfr-a; PDGFRA/BCR fusion; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein

View more View less

Gene Aliases: CD140A; GAS9; PDGFR-2; PDGFR2; PDGFRA; RHEPDGFRA

View more View less

UniProt ID: (Human) P16234

View more View less

Entrez Gene ID: (Human) 5156

View more View less

Function(s)
protein kinase activity transmembrane receptor protein tyrosine kinase activity platelet-derived growth factor alpha-receptor activity vascular endothelial growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding ATP binding vascular endothelial growth factor binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity platelet-derived growth factor binding transmembrane signal receptor
Process(es)
MAPK cascade luteinization cell activation positive regulation of cytosolic calcium ion concentration positive regulation of cell proliferation negative regulation of platelet activation positive regulation of phospholipase C activity regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling viral process peptidyl-tyrosine phosphorylation positive regulation of cell migration cellular response to reactive oxygen species platelet-derived growth factor receptor-alpha signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway wound healing positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication protein autophosphorylation phosphatidylinositol phosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis regulation of chemotaxis cardiac myofibril assembly cell chemotaxis retina vasculature development in camera-type eye positive regulation of ERK1 and ERK2 cascade platelet aggregation metanephric glomerular capillary formation regulation of actin cytoskeleton reorganization regulation of mesenchymal stem cell differentiation
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support