Invitrogen

ZO-1 Polyclonal Antibody

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This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

4 Published Figures

4 References

View all (43) ZO-1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

ZO-1 Antibody (PA5-28869) in ICC/IF

Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 Rabbit Polyclonal Antibody (Product # PA5-28869) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

Product Image Gallery

ZO-1 Antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Immunofluorescence of ZO-1 using ZO-1 Polyclonal Antibody (Product # PA5-28869) performed along with another ZO-1 antibody (Product # PA5-19090) shows similar localization pattern in Caco-2 cells. {IAV}

Product Details

PA5-28869

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 556 and 939 of Human ZO-1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.98 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546345

Product Specific Information

Recommended positive controls: HeLa, HCT116, HaCaT.

Predicted reactivity: Mouse (99%), Rat (99%), Dog (100%).

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peanut lipids influence the response of bronchial epithelial cells to the peanut allergens Ara h 1 and Ara h 2 by decreasing barrier permeability.

Frontiers in molecular biosciences

Palladino C,Ellinger I,Kalic T,Humeniuk P,Ret D,Mayr V,Hafner C,Hemmer W,Hoffmann-Sommergruber K,Untersmayr E,Bublin M,Radauer C,Breiteneder H

PA5-28869 was used in Immunocytochemistry-immunofluorescence to provides evidence of the transport of Ara h 1 and Ara h 2 across the airway epithelium, of the induction of a pro-inflammatory milieu, and identifies an important role for PNL in controlling the amount of allergens that can cross the epithelial barrier.

Tue Feb 28 00:00:00 EST 2023

Vardenafil increases intracellular accumulation of the most prevalent mutant cystic fibrosis transmembrane conductance regulator (CTFR) in human bronchial epithelial cells.

Biology open

Dhooghe B,Bouzin C,Mottais A,Hermans E,Delion M,Panin N,Noel S,Leal T

PA5-28869 was used in Immunocytochemistry-immunoflourescence to support the view that vardenafil partially rescues F508del through cGMP/PKG-independent mechanisms.

Tue Aug 25 00:00:00 EDT 2020

Vardenafil increases intracellular accumulation of the most prevalent mutant cystic fibrosis transmembrane conductance regulator (CTFR) in human bronchial epithelial cells.

Biology open

Dhooghe B,Bouzin C,Mottais A,Hermans E,Delion M,Panin N,Noel S,Leal T

PA5-28869 was used in Immunocytochemistry-immunoflourescence to support the view that vardenafil partially rescues F508del through cGMP/PKG-independent mechanisms.

Tue Aug 25 00:00:00 EDT 2020

Inhibition of MicroRNA-155 Supports Endothelial Tight Junction Integrity Following Oxygen-Glucose Deprivation.

Journal of the American Heart Association

Pena-Philippides JC,Gardiner AS,Caballero-Garrido E,Pan R,Zhu Y,Roitbak T

Published figure using ZO-1 polyclonal antibody (Product # PA5-28869) in Western Blot

Tue Jun 26 00:00:00 EDT 2018

Resveratrol increases F508del-CFTR dependent salivary secretion in cystic fibrosis mice.

Biology open

Dhooghe B,Bouckaert C,Capron A,Wallemacq P,Leal T,Noel S

Published figure using ZO-1 polyclonal antibody (Product # PA5-28869) in Immunofluorescence

Fri Jun 19 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

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Gene Aliases: TJP1; ZO-1; ZO1

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UniProt ID: (Human) Q07157

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Entrez Gene ID: (Human) 7082

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Function(s)

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion tight junction

Process(es)

blastocyst formation cell-cell junction assembly sensory perception of sound hippo signaling establishment of endothelial intestinal barrier cell-cell adhesion cell-cell signaling involved in cell-cell junction organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZO-1 Polyclonal Antibody

ZO-1 Antibody (PA5-28869) in ICC/IF

Product Details

PA5-28869

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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