Invitrogen

SIRT6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (26) SIRT6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SIRT6 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

SIRT6 Antibody (PA5-17215) in ICC/IF

Immunofluorescence analysis of SIRT6 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SIRT6 Polyclonal Antibody (Product # PA5-17215) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

Product Image Gallery

SIRT6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with SIRT6 siRNA and reduction of signal was observed in Western Blot using SIRT6 Polyclonal Antibody (Product # PA5-17215). {KD}

Product Details

PA5-17215

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

1:50

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the carboxy terminus of the human SirT6 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

16 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977684

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other sirtuin proteins.

Target Information

Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6), a mouse Sir2 homolog, is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in human aging.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Natural polyphenols as sirtuin 6 modulators.

Scientific reports

Rahnasto-Rilla M,Tyni J,Huovinen M,Jarho E,Kulikowicz T,Ravichandran S,A Bohr V,Ferrucci L,Lahtela-Kakkonen M,Moaddel R

Published figure using SIRT6 polyclonal antibody (Product # PA5-17215) in Western Blot

Wed Mar 07 00:00:00 EST 2018

Myeloid deletion of SIRT1 induces inflammatory signaling in response to environmental stress.

Molecular and cellular biology

Schug TT,Xu Q,Gao H,Peres-da-Silva A,Draper DW,Fessler MB,Purushotham A,Li X

PA5-17215 was used in ChIP assay to study the role of SIRT1 in the inflammatory/immune response.

Fri Oct 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: hSIRT6; NAD-dependent protein deacetylase sirtuin-6; NAD-dependent protein deacylase sirtuin-6; Protein mono-ADP-ribosyltransferase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2; SIR2 like 6; SIR2-like protein 6; sir2-related protein type 6; sirtuin type 6

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Gene Aliases: SIR2L6; SIRT6

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UniProt ID: (Human) Q8N6T7

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Entrez Gene ID: (Human) 51548

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Function(s)

chromatin binding transcription corepressor activity NAD+ ADP-ribosyltransferase activity NAD(P)+-protein-arginine ADP-ribosyltransferase activity protein binding zinc ion binding NAD-dependent histone deacetylase activity NAD-dependent protein deacetylase activity NAD-dependent histone deacetylase activity (H3-K9 specific) NAD+ binding

Process(es)

post-embryonic cardiac muscle cell growth involved in heart morphogenesis base-excision repair protein ADP-ribosylation negative regulation of cell proliferation regulation of double-strand break repair via homologous recombination protein destabilization response to nutrient levels glucose homeostasis negative regulation of glycolytic process negative regulation of transcription, DNA-templated negative regulation of glucose import positive regulation of fibroblast proliferation histone H3-K9 modification histone H3-K9 deacetylation positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SIRT6 Polyclonal Antibody

SIRT6 Antibody (PA5-17215) in ICC/IF

Product Details

PA5-17215

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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