Invitrogen

N-cadherin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

8 References

View all (97) N-cadherin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

N-cadherin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

N-cadherin Antibody (PA5-29570) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of N-cadherin was performed in NT2D1 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: N-cadherin Polyclonal Antibody (Product # PA5-29570) diluted at 1:500. Blue: Hoechst 33342 staining.

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

N-cadherin Antibody in Western Blot (WB)

N-cadherin Antibody in Immunohistochemistry (IHC)

N-cadherin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Product Details

PA5-29570

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 79 and 387 of Human N-cadherin

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547046

Product Specific Information

Recommended positive controls: NT2D1, U87-MG, SK-N-SH, IMR-32, SK-N-AS, mouse brain, rat brain, PC-12, Rat2.

Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (85%), Dog (96%), Chicken (91%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

Published figure using N-cadherin polyclonal antibody (Product # PA5-29570) in Western Blot

Wed Feb 02 00:00:00 EST 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

Published figure using N-cadherin polyclonal antibody (Product # PA5-29570) in Western Blot

Wed Feb 02 00:00:00 EST 2022

Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer Cells.

Molecules (Basel, Switzerland)

Wang H,Luo Y,Chu Z,Ni T,Ou S,Dai X,Zhang X,Liu Y

PA5-29570 was used in Western Blotting to show that Triterpenoids from P. cocos have significant biological activity against gastric cancer, and the mechanism may be involved in the process of epithelial-mesenchymal transformation.

Mon Jun 06 00:00:00 EDT 2022

Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer Cells.

Molecules (Basel, Switzerland)

Wang H,Luo Y,Chu Z,Ni T,Ou S,Dai X,Zhang X,Liu Y

Mon Jun 06 00:00:00 EDT 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

PA5-29570 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to conclude that CircATXN7 accelerated the malignancy of NSCLC cells through adsorbing miR-7-5p and upregulating PFN2, offering evidence to support circATXN7 as a target for NSCLC treatment.

Wed Jun 01 00:00:00 EDT 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

Wed Jun 01 00:00:00 EDT 2022

A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems.

Scientific reports

Wysmolek PM,Kiessler FD,Salbaum KA,Shelton ER,Sonntag SM,Serwane F

PA5-29570 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to create a 3D connectivity map of a stem cell derived neuron spheroid by imaging its activity.

Mon Nov 28 00:00:00 EST 2022

A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.

Nature communications

Quach C,Song Y,Guo H,Li S,Maazi H,Fung M,Sands N,O'Connell D,Restrepo-Vassalli S,Chai B,Nemecio D,Punj V,Akbari O,Idos GE,Mumenthaler SM,Wu N,Martin SE,Hagiya A,Hicks J,Cui H,Liang C

Published figure using N-cadherin polyclonal antibody (Product # PA5-29570) in Immunohistochemistry

Thu Dec 12 00:00:00 EST 2019

Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.

Nature communications

He S,Zhao Z,Yang Y,O'Connell D,Zhang X,Oh S,Ma B,Lee JH,Zhang T,Varghese B,Yip J,Dolatshahi Pirooz S,Li M,Zhang Y,Li GM,Ellen Martin S,Machida K,Liang C

PA5-29570 was used in immunohistochemistry to study the role of autophagy factors in cancer progression and treatment response

Mon Aug 03 00:00:00 EDT 2015

Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.

Nature communications

He S,Zhao Z,Yang Y,O'Connell D,Zhang X,Oh S,Ma B,Lee JH,Zhang T,Varghese B,Yip J,Dolatshahi Pirooz S,Li M,Zhang Y,Li GM,Ellen Martin S,Machida K,Liang C

PA5-29570 was used in immunohistochemistry to study the role of autophagy factors in cancer progression and treatment response

Mon Aug 03 00:00:00 EDT 2015

Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.

Nature communications

He S,Zhao Z,Yang Y,O'Connell D,Zhang X,Oh S,Ma B,Lee JH,Zhang T,Varghese B,Yip J,Dolatshahi Pirooz S,Li M,Zhang Y,Li GM,Ellen Martin S,Machida K,Liang C

PA5-29570 was used in immunohistochemistry to study the role of autophagy factors in cancer progression and treatment response

Mon Aug 03 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

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Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

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UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3

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Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501

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Function(s)

calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding enzyme binding metal ion binding cadherin

Process(es)

cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration cerebral cortex development regulation of myelination adherens junction organization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation positive regulation of muscle cell differentiation protein heterooligomerization radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering synapse assembly regulation of signal transduction single organismal cell-cell adhesion regulation of protein localization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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N-cadherin Polyclonal Antibody

N-cadherin Antibody (PA5-29570) in ICC/IF

Product Details

PA5-29570

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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