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Invitrogen
Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 615, eBioscience™
Product Details
42-4724-80
Immunohistochemistry (Paraffin) (IHC (P))
Immunocytochemistry (ICC/IF)
Control (Ctrl)
Host/Isotype
Class
Type
Clone
Conjugate
Excitation/Emission Max
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Purification
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RRID
Product Specific Information
Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies.
Applications Reported: This eBM2a antibody has been reported for use in immunohistochemical staining and immunocytochemistry.
Applications Tested: This eBM2a antibody has been tested by immunocytochemistry and immunohistochemical staining. Use isotype control at the same concentration as experimental antibody. This product has not been validated for flow cytometric analysis.
Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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