Invitrogen

MUSK Polyclonal Antibody

MUSK Antibody in Immunocytochemistry (ICC/IF)

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MUSK Antibody (PA1-1741) in ICC/IF

Immunofluorescent analysis of muscle-specific kinase (MuSK) (green) in C2C12 mouse myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody (Product # PA1-1741, right panel), at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary... View More

MUSK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA1-1741

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:100

Immunocytochemistry (ICC/IF)

1:20-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein containing residues 210-304 from the extracellular domain of rat MuSK

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2147253

Product Specific Information

PA1-1741 detects a predominant band at ~98kD. Other lower MW nonspecific bands of unknown identity were also detected and are likely to be proteolytic fragments.

Target Information

MUSK plays an important and selective role in many aspects of skeletal muscle development and function through the initial step of postsynaptic differentiation and formation of end-plate bands in cooperation with Rapsyn. Communication between cells is often mediated by receptors on the surface of the target cell that are triggered by specific ligands released by other cells. Receptor tyrosine kinase activity is strictly regulated by the binding of the appropriate ligand to the extracellular active site of the receptor. MuSK has been shown to be part of the agrin signaling complex and plays an important role in the development and architectural maintenance of the neuromuscular junction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction.

eLife

Afshar Bakooshli M,Lippmann ES,Mulcahy B,Iyer N,Nguyen CT,Tung K,Stewart BA,van den Dorpel H,Fuehrmann T,Shoichet M,Bigot A,Pegoraro E,Ahn H,Ginsberg H,Zhen M,Ashton RS,Gilbert PM

PA1-1741 was used in Immunocytochemistry-immunoflourescence to evaluate and model 3D human neuromuscular junction de novo development or disease in culture.

Tue May 14 00:00:00 EDT 2019

Interaction between ROR1 and MuSK activation complex in myogenic cells.

FEBS letters

Karvonen H,Summala K,Niininen W,Barker HR,Ungureanu D

PA1-1741 was used in Immunoprecipitation to identify MuSK as a ROR1 as a new interacting partner for MuSK and Dok-7, which may have an important role in myogenic cell signalling.

Thu Feb 01 00:00:00 EST 2018

Interaction between ROR1 and MuSK activation complex in myogenic cells.

FEBS letters

Karvonen H,Summala K,Niininen W,Barker HR,Ungureanu D

PA1-1741 was used in Immunoprecipitation to identify MuSK as a ROR1 as a new interacting partner for MuSK and Dok-7, which may have an important role in myogenic cell signalling.

Thu Feb 01 00:00:00 EST 2018

The Agrin/MuSK signaling pathway is spatially segregated from the neuregulin/ErbB receptor signaling pathway at the neuromuscular junction.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Trinidad JC,Fischbach GD,Cohen JB

PA1-1741 was used in immunohistochemistry to investigate the distribution of protein components of the neuregulin/erbB receptor and agrin/MuSK pathways at NMJs of adult rat gastrocnemius muscle

Fri Dec 01 00:00:00 EST 2000

Laminin and alpha-dystroglycan mediate acetylcholine receptor aggregation via a MuSK-independent pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Montanaro F,Gee SH,Jacobson C,Lindenbaum MH,Froehner SC,Carbonetto S

PA1-1741 was used in immunoprecipitation to investigate the regulation of AChR aggregation by laminin and alpha-dystroglycan

Sun Feb 15 00:00:00 EST 1998

Dimerization of the muscle-specific kinase induces tyrosine phosphorylation of acetylcholine receptors and their aggregation on the surface of myotubes.

The Journal of biological chemistry

Hopf C,Hoch W

PA1-1741 was used in western blot to investigate the effect of MuSK dimerization on acetylcholine receptor's tyrosine phosphorylation and aggregation

Fri Mar 13 00:00:00 EST 1998

Bioinformatics

Protein Aliases: MGC126323; MGC126324; muscle localized kinase 2; muscle specific kinase (neural fold/somite kinase 1); Muscle, skeletal receptor tyrosine protein kinase; Muscle, skeletal receptor tyrosine-protein kinase; muscle, skeletal, receptor tyrosine kinase; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine kinase receptor MuSK; Muscle-specific tyrosine protein kinase receptor; Muscle-specific tyrosine-protein kinase receptor; MuSK; RP23-170A3.1

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Gene Aliases: CMS9; FADS; Mdk4; Mlk; MUSK; Nsk1; Nsk2; Nsk3

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UniProt ID: (Human) O15146, (Mouse) Q61006, (Rat) Q62838

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Entrez Gene ID: (Human) 4593, (Mouse) 18198, (Rat) 81725

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Function(s)

protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding metal ion binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding PDZ domain binding transmembrane signal receptor

Process(es)

positive regulation of protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development neuromuscular junction development memory regulation of synaptic growth at neuromuscular junction positive regulation of gene expression peptidyl-tyrosine phosphorylation cell differentiation protein autophosphorylation skeletal muscle acetylcholine-gated channel clustering positive regulation of protein geranylgeranylation regulation of transcription, DNA-templated protein phosphorylation signal transduction negative regulation of gene expression phosphorylation positive regulation of synaptic transmission, cholinergic receptor clustering positive regulation of neuron apoptotic process positive regulation of synaptic growth at neuromuscular junction positive regulation of peptidyl-tyrosine phosphorylation response to electrical stimulus long-term synaptic potentiation response to muscle activity response to axon injury retina development in camera-type eye cochlea development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MUSK Polyclonal Antibody

MUSK Antibody (PA1-1741) in ICC/IF

Product Details

PA1-1741

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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