Invitrogen

CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 700

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

5 Published Figures

6 References

View all (407) CD45 antibodies

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Datasheet

Protocols

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FIGURE: 1 / 6

CD45 Antibody (MHCD4529) in Flow

Nature 2018 - Published figure using CD45 monoclonal antibody (Product # MHCD4529) in Flow Cytometry

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD4529

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HI30

Immunogen

CD45 antigen

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373857

Product Specific Information

The Alexa Fluor® 700 dye conjugate provides an excellent spectral match to the common long-wavelength excitation sources, with a high extinction coefficient.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

ZNF384 Fusion Oncoproteins Drive Lineage Aberrancy in Acute Leukemia.

Blood cancer discovery

Dickerson KM,Qu C,Gao Q,Iacobucci I,Gu Z,Yoshihara H,Backhaus EA,Chang Y,Janke LJ,Xu B,Wu G,Papachristou EK,D'Santos CS,Roberts KG,Mullighan CG

MHCD4529 was used in Flow cytometry/Cell sorting to reveal the interplay between cell of origin and expression of ZNF384 FO to mediate lineage ambiguity and leukemia development.

Thu May 05 00:00:00 EDT 2022

The genetic basis and cell of origin of mixed phenotype acute leukaemia.

Nature

Alexander TB,Gu Z,Iacobucci I,Dickerson K,Choi JK,Xu B,Payne-Turner D,Yoshihara H,Loh ML,Horan J,Buldini B,Basso G,Elitzur S,de Haas V,Zwaan CM,Yeoh A,Reinhardt D,Tomizawa D,Kiyokawa N,Lammens T,De Moerloose B,Catchpoole D,Hori H,Moorman A,Moore AS,Hrusak O,Meshinchi S,Orgel E,Devidas M,Borowitz M,Wood B,Heerema NA,Carrol A,Yang YL,Smith MA,Davidsen TM,Hermida LC,Gesuwan P,Marra MA,Ma Y,Mungall AJ,Moore RA,Jones SJM,Valentine M,Janke LJ,Rubnitz JE,Pui CH,Ding L,Liu Y,Zhang J,Nichols KE,Downing JR,Cao X,Shi L,Pounds S,Newman S,Pei D,Guidry Auvil JM,Gerhard DS,Hunger SP,Inaba H,Mullighan CG

MHCD4529 was used in Flow cytometry/Cell sorting to show that the two principal subtypes of mixed phenotype acute leukaemia, T/myeloid and B/myeloid, are genetically distinct.

Mon Oct 01 00:00:00 EDT 2018

The genetic basis and cell of origin of mixed phenotype acute leukaemia.

Nature

MHCD4529 was used in Flow cytometry/Cell sorting to show that the two principal subtypes of mixed phenotype acute leukaemia, T/myeloid and B/myeloid, are genetically distinct.

Mon Oct 01 00:00:00 EDT 2018

Chronic stress is associated with reduced circulating hematopoietic progenitor cell number: A maternal caregiving model.

Brain, behavior, and immunity

Aschbacher K,Milush JM,Gilbert A,Almeida C,Sinclair E,Epling L,Grenon SM,Marco EJ,Puterman E,Epel E

MHCD4529 was used in Flow cytometry/Cell sorting to determine the role of circulating hematopoeitic progenitor cells on chronic psychological stress.

Sun Jan 01 00:00:00 EST 2017

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Mucosal immunology

Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M

MHCD4529 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.

Fri Jan 01 00:00:00 EST 2016

Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels^¿.

Biotechnology reports (Amsterdam, Netherlands)

Sun Z,Lawson DA,Sinclair E,Wang CY,Lai MD,Hetts SW,Higashida RT,Dowd CF,Halbach VV,Werb Z,Su H,Cooke DL

Published figure using CD45 monoclonal antibody (Product # MHCD4529) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Long BR,Stoddart CA

Published figure using CD45 monoclonal antibody (Product # MHCD4529) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 700

CD45 Antibody (MHCD4529) in Flow

Product Details

MHCD4529

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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