Invitrogen

MAP2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

2 References

View all (71) MAP2 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

MAP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

MAP2 Antibody (PA5-85755) in ICC/IF

Immunofluorescence analysis of Microtubule-associated protein 2 was performed using 70% confluent log phase SH-SY5Y cells differentiated to neurons (Retinoic acid 10 uM for 5 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MAP2 Polyclonal Antibody (Product # PA5-85755) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit Ig... View More

MAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MAP2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

MAP2 Antibody in Immunohistochemistry (IHC)

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MAP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Increased expression of Microtubule-associated protein 2 was observed in SH-SY5Y following its differentiation into neurons, using Anti-MAP2 Polyclonal Antibody (Product # PA5-85755) in Western Blot. The same antibody was used to observe higher expression levels of MAP2 in IMR-32 against BJ and BeWo. {RE}

Product Details

PA5-85755

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rhesus monkey

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Mouse MAP2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.07 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792892

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%).

Positive Control: mouse brain, DIV9 rat E18 primary cortical neurons.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular Vesicle Treatment Alleviates Neurodevelopmental and Neurodegenerative Pathology in Cortical Spheroid Model of Down Syndrome.

International journal of molecular sciences

Campbell NB,Patel Y,Moore TL,Medalla M,Zeldich E

PA5-85755 was used in Immunohistochemistry (Frozen) to show the efficacy of EVs in mitigating DS and AD-related cellular phenotypes and pathological depositions in human CS.

Thu Feb 09 00:00:00 EST 2023

Extracellular Vesicle Treatment Alleviates Neurodevelopmental and Neurodegenerative Pathology in Cortical Spheroid Model of Down Syndrome.

International journal of molecular sciences

Campbell NB,Patel Y,Moore TL,Medalla M,Zeldich E

PA5-85755 was used in Immunohistochemistry (Frozen) to show the efficacy of EVs in mitigating DS and AD-related cellular phenotypes and pathological depositions in human CS.

Thu Feb 09 00:00:00 EST 2023

Bioinformatics

Protein Aliases: AC091465.1; DKFZp686I2148; MAP-2; map-2a; map2ab; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143

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Gene Aliases: G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4

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UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146

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Entrez Gene ID: (Human) 4133, (Mouse) 17756, (Rat) 25595

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Function(s)

dystroglycan binding structural molecule activity protein binding calmodulin binding microtubule binding cytoskeletal regulatory protein binding tubulin binding

Process(es)

microtubule bundle formation axonogenesis dendrite development cellular response to organic substance microtubule cytoskeleton organization central nervous system neuron development neuron projection development dendrite morphogenesis response to estradiol response to progesterone

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MAP2 Polyclonal Antibody

MAP2 Antibody (PA5-85755) in ICC/IF

Product Details

PA5-85755

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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