Invitrogen

HSP90 Monoclonal Antibody (D7A), APC

View all (35) HSP90 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45157

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100,000
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

5 µg
-

Antibody Microarray (AM)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

D7A

Immunogen

Full length protein HSP90 purified from chicken brain
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

47.35mM phosphate/2.15mM MES, pH 7.2, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931611

Product Specific Information

2 µg/mL was sufficient for detection of HSP90 alpha in 20 µg of heat shocked HeLa cell lysate as well as in 100 ng of human HSP90 alpha protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Recognizes 90kDa.approximately Can isolate complexes of HSP90, Src kinase and cec37.

Target Information

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 90-kDa heat shock protein; epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 84 kDa; Heat shock 86 kDa; heat shock 90 kDa protein; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein 1, beta; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, alpha; heat shock protein 1, beta; heat shock protein 86; heat shock protein 90 beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein 90kDa alpha family class B member 1; Heat shock protein family C member 1; Heat shock protein family C member 3; Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta; heat shock protein HSP90 alpha; heat shock protein, 1; heat shock protein, 84 kDa 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; HSP; HSP 84; HSP 86; HSP 90; HSP84; HSP86; hsp90 beta; HSP90-beta; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; retinal degeneration slow protein; TSTA; Tumor-specific transplantation 84 kDa antigen; Tumor-specific transplantation 86 kDa antigen

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Gene Aliases: 86kDa; 89kDa; 90kDa; AL022974; AL024080; AL024147; C81438; D6S182; EL52; HEL-S-65p; hsp4; HSP84; Hsp84-1; HSP86; Hsp86-1; Hsp89; HSP89A; HSP90; HSP90-BETA; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSP90N; HSPC1; HSPC2; HSPC3; HSPCA; HSPCAL1; HSPCAL4; HSPCB; HSPN; LAP-2; LAP2

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UniProt ID: (Chicken) P11501, (Bovine) Q76LV2, (Pig) O02705, (Human) P07900, (Bovine) Q76LV1, (Rabbit) P30947, (Human) P08238, (Mouse) P07901, (Rat) P82995, (Mouse) P11499, (Rat) P34058

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Entrez Gene ID: (Chicken) 423463, (Bovine) 281832, (Pig) 397028, (Human) 3320, (Rabbit) 100338694, (Bovine) 767874, (Rabbit) 100358690, (Human) 3326, (Chicken) 396188, (Pig) 396742, (Mouse) 15519, (Rat) 299331, (Mouse) 15516, (Rat) 301252

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Function(s)
ATP binding protein C-terminus binding ATPase activity nitric-oxide synthase regulator activity estrogen receptor binding TPR domain binding heat shock protein binding progesterone receptor binding identical protein binding virion binding unfolded protein binding chaperone binding Hsp90 protein binding disordered domain specific binding protein binding protein homodimerization activity nucleotide binding UTP binding CTP binding mRNA binding protein tyrosine kinase activity GTP binding sulfonylurea receptor binding protein kinase binding protein phosphatase binding MHC class II protein complex binding dATP binding histone deacetylase binding ion channel binding poly(A) RNA binding GTPase binding Rho GDP-dissociation inhibitor binding double-stranded RNA binding protein kinase regulator activity ubiquitin protein ligase binding peptide binding ATP-dependent protein binding protein binding involved in protein folding protein dimerization activity tau protein binding DNA polymerase binding histone methyltransferase binding kinase binding cadherin binding involved in cell-cell adhesion Hsp90 family chaperone
Process(es)
protein folding positive regulation of cell proliferation response to heat response to cold response to organonitrogen compound response to organic cyclic compound response to progesterone cellular response to heat positive regulation of nitric oxide biosynthetic process response to antibiotic regulation of catalytic activity protein stabilization positive regulation of cell cycle G2/M phase transition G2/M transition of mitotic cell cycle neuron migration skeletal muscle contraction mitochondrial transport receptor-mediated endocytosis response to unfolded protein signal transduction response to salt stress positive regulation of lamellipodium assembly cardiac muscle cell apoptotic process peptidyl-tyrosine phosphorylation regulation of protein ubiquitination positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway protein refolding regulation of protein complex assembly protein unfolding response to estrogen protein import into mitochondrial outer membrane positive regulation of cell size vascular endothelial growth factor receptor signaling pathway regulation of nitric-oxide synthase activity chaperone-mediated protein complex assembly positive regulation of cardiac muscle contraction chaperone-mediated autophagy regulation of cellular response to heat placenta development telomere maintenance via telomerase virion attachment to host cell central nervous system neuron axonogenesis establishment of cell polarity positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of phosphoprotein phosphatase activity positive regulation of peptidyl-serine phosphorylation negative regulation of apoptotic process positive regulation of cell differentiation axon extension positive regulation of protein kinase B signaling positive regulation of telomerase activity regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway negative regulation of cell cycle arrest cellular response to interleukin-4 fibril organization negative regulation of transforming growth factor beta activation positive regulation of cyclin-dependent protein kinase activity telomerase holoenzyme complex assembly positive regulation of protein localization to cell surface positive regulation of protein binding negative regulation of neuron apoptotic process cellular response to organic cyclic compound positive regulation of protein serine/threonine kinase activity cell-cell adhesion nitric oxide biosynthetic process response to stress positive regulation of cytotoxic T cell differentiation
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