Invitrogen

CENPA Monoclonal Antibody (H.577.2)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (23) CENPA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CENPA Antibody in Immunocytochemistry (ICC/IF)

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CENPA Antibody (MA5-14829) in ICC/IF

Immunofluorescence analysis of CENPA was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (H.577.2) (Product # MA5-14829) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More

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CENPA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. NIH/3T3 cells were transfected with CENPA siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CENPA Monoclonal Antibody (H.577.2) (Product # MA5-14829). {KD}

Product Details

MA5-14829

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:200

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

H.577.2

Immunogen

Synthetic peptide corresponding to the amino terminus of mouse CENP-A protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

23.4 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10980200

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other histone proteins, including Histone H3.

Target Information

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CENP A; CENP-A; Centromere protein A; Histone H3-like centromeric protein A

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Gene Aliases: Cenp-A; Cenpa

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UniProt ID: (Mouse) O35216

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Entrez Gene ID: (Mouse) 12615

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Function(s)

histone

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CENPA Monoclonal Antibody (H.577.2)

CENPA Antibody (MA5-14829) in ICC/IF

Product Details

MA5-14829

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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