Invitrogen

SNAIL Monoclonal Antibody (F.31.8)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

10 References

View all (36) SNAIL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SNAIL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

SNAIL Antibody (MA5-14801) in ICC/IF

Immunofluorescence analysis of SNAIL was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SNAIL Monoclonal Antibody (F.31.8) (Product # MA5-14801) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2817... View More

Product Image Gallery

SNAIL Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HCT 116 cells were transfected with SNAIL siRNA and loss of signal was observed in Western Blot using SNAIL Monoclonal Antibody (F.31.8) (Product # MA5-14801). {KD}

Product Details

MA5-14801

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.31.8

Immunogen

Recombinant human Snail protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

110 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10981481

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of stemness-related genes TIMP1, PGF, and SNAI1 in the prognosis of colorectal cancer through single-cell RNA-seq.

Cancer medicine

Shen Y,Ni S,Li S,Lv B

MA5-14801 was used in Western Blot to analyze stemness-related prognostic genes of CRC based on single-cell RNA-sequencing (scRNA-seq) data.

Mon May 01 00:00:00 EDT 2023

Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

International journal of molecular sciences

Huang GK,Huang CC,Kang CH,Cheng YT,Tsai PC,Kao YH,Chung YH

MA5-14801 was used in Immunocytochemistry-immunofluorescence to profile the expression of FGFR3 in Asian and Caucasian UTUC tissues and to evaluate the in vitro therapeutic efficacy of small interference RNA (siRNA)-mediated FGFR3 silencing in UTUC treatment.

Mon Jan 16 00:00:00 EST 2023

Tbx18 Orchestrates Cytostructural Transdifferentiation of Cardiomyocytes to Pacemaker Cells by Recruiting the Epithelial-Mesenchymal Transition Program.

Journal of proteome research

Foster DB,Gu JM,Kim EH,Wolfson DW,O'Meally R,Cole RN,Cho HC

MA5-14801 was used in Western Blot to demonstrate that heterologous expression of an embryonic transcription factor, Tbx18, reprograms ventricular cardiomyocytes into induced pacemaker cells (Tbx18-iPMs), though the key pathways are unknown.

Fri Oct 07 00:00:00 EDT 2022

Silibinin Overcomes EMT-Driven Lung Cancer Resistance to New-Generation ALK Inhibitors.

Cancers

Verdura S,Encinar JA,Teixidor E,Segura-Carretero A,Micol V,Cuyàs E,Bosch-Barrera J,Menendez JA

MA5-14801 was used in Western Blotting to investigate whether first-generation ALK-TKI therapy-induced EMT promotes cross-resistance to new-generation ALK-TKIs and whether this could be circumvented by the flavonolignan silibinin, an EMT inhibitor.

Sun Dec 11 00:00:00 EST 2022

Adiponectin Suppresses Metastasis of Nasopharyngeal Carcinoma through Blocking the Activation of NF-κB and STAT3 Signaling.

International journal of molecular sciences

Zhang Z,Du J,Xu Q,Xing C,Li Y,Zhou S,Zhao Z,Mu Y,Zhao ZA,Cao S,Li F

MA5-14801 was used in Western Blotting to demonstrate that adiponectin could not only regulate metabolism and inhibit cancer growth, but also suppress the metastasis of NPC.

Sat Oct 22 00:00:00 EDT 2022

LINC00461 facilitates HNSCC development and reduces chemosensitivity by impairing miR-195-mediated inhibition of HOXA10.

Molecular therapy oncolytics

Guan Y,Guan A,Chen L,Gong A

MA5-14801 was used in Immunocytochemistry to demonstrate that LINC00461 modulates the expression of HOXA10 by regulating micro-RNA-195 to affect the epithelial-to-mesenchymal transition.

Fri Jun 25 00:00:00 EDT 2021

LINC00461 facilitates HNSCC development and reduces chemosensitivity by impairing miR-195-mediated inhibition of HOXA10.

Molecular therapy oncolytics

Guan Y,Guan A,Chen L,Gong A

MA5-14801 was used in Immunocytochemistry to demonstrate that LINC00461 modulates the expression of HOXA10 by regulating micro-RNA-195 to affect the epithelial-to-mesenchymal transition.

Fri Jun 25 00:00:00 EDT 2021

Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma.

Journal of chemotherapy (Florence, Italy)

Li SL,Jiang TQ,Cao QW,Liu SM

Published figure using SNAIL monoclonal antibody (Product # MA5-14801) in Western Blot

Mon Feb 01 00:00:00 EST 2021

CHD4 Predicts Aggressiveness in PTC Patients and Promotes Cancer Stemness and EMT in PTC Cells.

International journal of molecular sciences

Pratheeshkumar P,Siraj AK,Divya SP,Parvathareddy SK,Alobaisi K,Al-Sobhi SS,Al-Dayel F,Al-Kuraya KS

Published figure using SNAIL monoclonal antibody (Product # MA5-14801) in Western Blot

Wed Jan 06 00:00:00 EST 2021

Loss of ZNF677 Expression Is an Independent Predictor for Distant Metastasis in Middle Eastern Papillary Thyroid Carcinoma Patients.

International journal of molecular sciences

Siraj AK,Poyil PK,Parvathareddy SK,Alobaisi K,Ahmed SO,Al-Sobhi SS,Al-Dayel F,Al-Kuraya KS

Published figure using SNAIL monoclonal antibody (Product # MA5-14801) in Western Blot

Thu Jul 22 00:00:00 EDT 2021

Diversity in the Extracellular Vesicle-Derived Microbiome of Tissues According to Tumor Progression in Pancreatic Cancer.

Cancers

Jeong JY,Kim TB,Kim J,Choi HW,Kim EJ,Yoo HJ,Lee S,Jun HR,Yoo W,Kim S,Kim SC,Jun E

MA5-14801 was used in Western Blotting to investigate the role, composition and diversity of the microbiome in tissues of pancreatic cancer.

Wed Aug 19 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1

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Gene Aliases: AI194338; dJ710H13.1; SLUGH2; SNA; Sna1; SNAH; SNAI1; SNAIL; SNAIL1

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UniProt ID: (Human) O95863, (Mouse) Q02085

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Entrez Gene ID: (Human) 6615, (Mouse) 20613, (Rat) 116490

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding kinase binding metal ion binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding C2H2 zinc finger transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation mesoderm formation epithelial to mesenchymal transition epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition negative regulation of vitamin D biosynthetic process cell migration positive regulation of cell migration hair follicle morphogenesis negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of transcription, DNA-templated palate development cartilage morphogenesis trophoblast giant cell differentiation negative regulation of cell differentiation involved in embryonic placenta development left/right pattern formation Notch signaling involved in heart development negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage regulation of bicellular tight junction assembly regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development mesoderm development negative regulation of transcription, DNA-templated negative regulation of cell cycle

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SNAIL Monoclonal Antibody (F.31.8)

SNAIL Antibody (MA5-14801) in ICC/IF

Product Details

MA5-14801

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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