Invitrogen

14-3-3 beta/epsilon/zeta Monoclonal Antibody (3C8)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (3) 14-3-3 Pan antibodies

Datasheet

Protocols

Datasheet

Protocols

14-3-3 beta/epsilon/zeta Antibody in Western Blot (WB)

FIGURE: 1 / 5

14-3-3 beta/epsilon/zeta Antibody (MA1-34561) in WB

Western blot was performed using Anti-14-3-3 beta/epsilon/zeta Monoclonal Antibody (3C8) (Product # MA1-34561) and a 28 kDa band corresponding to 14-3-3 protein beta/epsilon/zeta was observed across the cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), PC-3 (Lane 3), A549 (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6), Mouse Bra... View More

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Product Details

MA1-34561

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

Product Specifications

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3C8

Immunogen

Human 14-3-3 beta fusion protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1954768

Product Specific Information

Recommended positive controls: HeLa Cell Lysate, Mouse Brain Tissue Extract, Rat Brain Tissue Extract.

Target Information

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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