Invitrogen

FAS Monoclonal Antibody (UT-1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (107) FAS (CD95) antibodies

Datasheet

Protocols

Datasheet

Protocols

FIGURE: 1 / 2

FAS Antibody (MA1-19024) in WB

Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of Jurkat (Lane 1), Raji (Lane 2), MOLT-4 (Lane 3), HeLa (Lane 4), MCF7 (Lane 5) and SW-480 (Lane 6). The blot was probed with anti-FAS Monoclonal Antibody (Product # MA1-19024, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 31 kDa band corresponding to FAS receptor was observed across all the cell lines tested. HeLa, MCF7 and SW-4... View More

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FAS Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of FAS receptor was observed in cells of lymphoid origin (Jurkat, Raji and Molt4) showing higher expression in comparison to epithelial cell lines (HeLa, MCF7 and SW-480) using product (Product # MA1-19024) in western blot. {RE}

Product Details

MA1-19024

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Flow Cytometry (Flow)

2-10 µg/mL

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

UT-1

Immunogen

T cell lymphoma cells HUT-78.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_1073502

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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