Invitrogen

JNK1 Monoclonal Antibody (1E5)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-17117

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E5

Immunogen

Purified recombinant fragment of human MAPK8 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538588

Product Specific Information

MA5-17117 targets MAPK8 in FACS, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17117 immunogen is purified recombinant fragment of human MAPK8 expressed in E. Coli.

MA5-17117 detects MAPK8 which has a predicted molecular weight of approximately 48.3kDa.

Target Information

MAPK8 (JNK1) is activated by the presence of misfolded proteins in the endoplasmic reticulum. It is also a crucial mediator of insulin resistance and obesity. It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-Jun N-terminal kinase 1; FLJ12099; FLJ33785; JNK-46; JNK1 beta1 protein kinase; JNK3; JNK3A; JUN N-terminal kinase; MAP kinase 8; MAPK 10; MAPK 8; MAPK10; MGC50974; mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; MK10; OTTHUMP00000019552; p493F12; p54bSAPK; Phospho-JNK; PRKM10; protein kinase JNK1; protein kinase mitogen-activated 8; SAPK1c; stress-activated protein kinase 1; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1

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Gene Aliases: AI849689; JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; MAPK8; PRKM8; SAPK1; SAPK1C

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UniProt ID: (Human) P45983, (Mouse) Q91Y86

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Entrez Gene ID: (Human) 5599, (Mouse) 26419

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Function(s)
protein serine/threonine kinase activity JUN kinase activity protein binding ATP binding kinase activity enzyme binding histone deacetylase regulator activity histone deacetylase binding nucleotide binding protein kinase activity MAP kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
protein phosphorylation response to stress JNK cascade JUN phosphorylation response to UV positive regulation of gene expression regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation regulation of histone deacetylation positive regulation of cyclase activity negative regulation of protein binding regulation of protein localization Fc-epsilon receptor signaling pathway regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process rhythmic process regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein metabolic process cellular response to lipopolysaccharide cellular response to mechanical stimulus positive regulation of deacetylase activity positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway ossification regulation of transcription, DNA-templated inflammatory response response to osmotic stress signal transduction response to heat regulation of gene expression phosphorylation positive regulation of cell migration positive regulation of microtubule polymerization neuron projection development response to hydrogen peroxide positive regulation of DNA replication response to cadmium ion determination of dorsal identity cellular response to hydrogen peroxide cellular response to nitric oxide type B pancreatic cell apoptotic process neuronal stem cell population maintenance programmed necrotic cell death positive regulation of determination of dorsal identity positive regulation of apoptotic signaling pathway
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