Invitrogen

Influenza A PA Polyclonal Antibody

Influenza A PA Antibody in Immunocytochemistry (ICC/IF)

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Influenza A PA Antibody (PA5-31315) in ICC/IF

Influenza A Virus PA antibody detects polymerase PA (influenza A H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: polymerase PA (influenza A H1N1) protein stained by Influenza A Virus PA antibody (Product # PA5-31315) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C,30 min.

Influenza A PA Antibody in Western Blot (WB)

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Product Details

PA5-31315

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 465 and 746 of H1N1 Influenza A Virus PA

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.91 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2548789

Product Specific Information

Recommended positive controls: Influenza virus- infected H1299 lysates, Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates, influenza virus (A/WSN/33) infected MDCK cells.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Influenza viruses consists of three types (Influenza A, B, and C), which make up three of the five genera in the Orthomyxoviridae family. Commonly know as the flu, influenza strains can affect both birds and mammals. Influenza strains form roughly spherical particles and are similar in composition with viral envelope containing two main types of glycoproteins (hemagglutinin and neuraminidase), wrapped around the central core of the viral RNA genome and other viral proteins that package and protect the RNA. Unusually for a virus, the influenzavirus genome is not a single piece of nucleic acid; rather a seven to eight segmented fragments of negative-sense RNA with each fragment expressing different viral proteins. Influenza spreads seasonally, resulting in about three to five million yearly cases of severe illness and about 250,000 to 500,000 deaths annually or into the millions in pandemic years. Influenza A virus contains eight single stranded, negative sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase which consists of PA, PB1 and PB2 proteins. Recent reports indicate the involvement of PA in 'cap-snatching' mechanism via its endonuclease activity which is essential for cRNA-dependent viral RNA synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Influenza PA

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