Invitrogen

ITGB1 Monoclonal Antibody (3B6)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures
3 References
View all (125) Integrin beta 1 (CD29) antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-17103

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3B6

Immunogen

Purified recombinant fragment of human ITGB1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538574

Product Specific Information

MA5-17103 targets ITGB1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-17103 immunogen is purified recombinant fragment of human ITGB1 expressed in E. Coli.

MA5-17103 detects ITGB1 which has a predicted molecular weight of approximately 88.4kDa.

Target Information

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; very late activation protein, beta polypeptide; VLA-4 subunit beta

View more View less

Gene Aliases: 4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; FNRB; Gm9863; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

View more View less

UniProt ID: (Human) P05556, (Mouse) Q8BVU1

View more View less

Entrez Gene ID: (Human) 3688, (Mouse) 16412

View more View less

Function(s)
virus receptor activity glycoprotein binding fibronectin binding protease binding actin binding integrin binding protein binding coreceptor activity protein kinase binding protein domain specific binding C-X3-C chemokine binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion cadherin binding involved in cell-cell adhesion receptor activity receptor binding collagen binding kinase binding integrin
Process(es)
G1/S transition of mitotic cell cycle in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis cellular calcium ion homeostasis cellular defense response homophilic cell adhesion via plasma membrane adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning regulation of collagen catabolic process positive regulation of cell-substrate adhesion positive regulation of neuron projection development response to activity cell migration formation of radial glial scaffolds B cell differentiation extracellular matrix organization positive regulation of cell migration negative regulation of cell projection organization cell-substrate adhesion receptor internalization protein transport within lipid bilayer cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion response to gonadotropin negative regulation of Rho protein signal transduction response to drug positive regulation of apoptotic process stress fiber assembly positive regulation of MAPK cascade positive regulation of GTPase activity sarcomere organization negative regulation of neuron differentiation positive regulation of endocytosis viral entry into host cell mesodermal cell differentiation axon extension dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response leukocyte migration leukocyte tethering or rolling regulation of cell cycle cardiac muscle cell differentiation maternal process involved in female pregnancy bicellular tight junction assembly cellular response to mechanical stimulus cellular response to vitamin D cellular response to low-density lipoprotein particle stimulus cellular response to ionizing radiation positive regulation of establishment of protein localization to plasma membrane negative regulation of anoikis cell adhesion neuron projection development negative regulation of cell differentiation positive regulation of neuron differentiation cardiac muscle tissue development
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support