Invitrogen

IRF4 Monoclonal Antibody (3E4), PE, eBioscience™

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IRF4 Antibody (12-9858-82) in Flow

Normal human peripheral blood cells were either unstimulated (blue historgam) or stimulated for 3 days with Anti-Human CD3 (Product # 16-0037-81) and Anti-Human CD28 (Product # 16-0289-81) (purple histogram). Cells were then fixed and permeabilized with the Foxp3 Fixation/Permeabilization Set (Product # 00-5523-00) and stained with Anti-Human CD4 FITC (Product # 11-0049-42) and 0.06 µg Anti-Human/Mouse IRF4 PE. CD4 positive cells were used for analysis and staining of stimulated cells with Rat IgG1 K Isotype Control PE (Product # 12-4301-8... View More

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Product Details

12-9858-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3E4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10852721

Product Specific Information

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), Th2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.

Applications Reported:This 3E4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 3E4 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids.

ACS central science

Moeller TD,Shah SB,Lai K,Lopez-Barbosa N,Desai P,Wang W,Zhong Z,Redmond D,Singh A,DeLisa MP

12-9858-82 was used in Flow cytometry/Cell sorting to highlight the potential of synthetic organoids for rapidly predicting conjugate vaccine efficacy as well as expediting antigen-specific antibody discovery.

Wed Apr 26 00:00:00 EDT 2023

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

12-9858-82 was used in Flow Cytometry to show that interleukin-27 production by B cells contributes to their impact on primary, but not memory, CD8 responses.

Tue Aug 24 00:00:00 EDT 2021

The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice.

eLife

Lehrke MJ,Shapiro MJ,Rajcula MJ,Kennedy MM,McCue SA,Medina KL,Shapiro VS

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Thu Nov 11 00:00:00 EST 2021

Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans.

Immunity

Cytlak U,Resteu A,Pagan S,Green K,Milne P,Maisuria S,McDonald D,Hulme G,Filby A,Carpenter B,Queen R,Hambleton S,Hague R,Lango Allen H,Thaventhiran JED,Doody G,Collin M,Bigley V

12-9858 was used in Flow cytometry/Cell sorting to demonstrate that cDC2 (CD1c+DC) heterogeneity originates from two distinct pathways of development.

Tue Aug 18 00:00:00 EDT 2020

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

The Amount of BCL6 in B Cells Shortly after Antigen Engagement Determines Their Representation in Subsequent Germinal Centers.

Cell reports

Robinson MJ,Ding Z,Pitt C,Brodie EJ,Quast I,Tarlinton DM,Zotos D

12-9858-82 was used in Flow Cytometry to propose that setting BCL6 amounts very early in activated B cells will be central in determining clonal representation in the GC and thus memory populations.

Tue Feb 04 00:00:00 EST 2020

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

Noc4L-Mediated Ribosome Biogenesis Controls Activation of Regulatory and Conventional T Cells.

Cell reports

Zhu X,Zhang W,Guo J,Zhang X,Li L,Wang T,Yan J,Zhang F,Hou B,Gao N,Gao GF,Zhou X

12-9858 was used in Flow cytometry/Cell sorting to identify the control of Treg activation and immune tolerance maintenance is due to Noc4L mediated ribosome biogenesis.

Tue Apr 23 00:00:00 EDT 2019

Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction.

Immunity

Li X,Gadzinsky A,Gong L,Tong H,Calderon V,Li Y,Kitamura D,Klein U,Langdon WY,Hou F,Zou YR,Gu H

12-9858-82 was used in Flow Cytometry to show that deleting E3 ubiquitin ligases Cbl and Cbl-b (Cbls) in GC B cells results in the early exit of high-affinity antigen-specific B cells from the GC reaction and thus impaired clonal expansion.

Tue Mar 20 00:00:00 EDT 2018

CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development.

Frontiers in immunology

Cobbold SP,Adams E,Howie D,Waldmann H

12-9858 was used in Flow cytometry/Cell sorting to develop an in vitro model of T cell differentiation suited to testing how manipulation of components of the microenvironment might be therapeutically exploited.

Mon Jan 31 00:00:00 EST 2022

ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cells.

Scientific reports

Chen W,Nyuydzefe MS,Weiss JM,Zhang J,Waksal SD,Zanin-Zhorov A

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Fri Nov 09 00:00:00 EST 2018

Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3⁺RORγt⁺ Regulatory T Cells.

Cell reports

Ogawa C,Bankoti R,Nguyen T,Hassanzadeh-Kiabi N,Nadeau S,Porritt RA,Couse M,Fan X,Dhall D,Eberl G,Ohnmacht C,Martins GA

12-9858-82 was used in Flow Cytometry to study the role of Blimp-1 as a crucial regulator of the Foxp3+RORγt+ regulatory T cell subset.

Tue Oct 02 00:00:00 EDT 2018

Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF-IRF4 transcription factor complex.

Nature immunology

Iwata A,Durai V,Tussiwand R,Briseño CG,Wu X,Grajales-Reyes GE,Egawa T,Murphy TL,Murphy KM

Mon May 01 00:00:00 EDT 2017

Batf3 selectively determines acquisition of CD8⁺ dendritic cell phenotype and function.

Immunology and cell biology

Chandra J,Kuo PT,Hahn AM,Belz GT,Frazer IH

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

γ-Tocopherol supplementation of allergic female mice augments development of CD11c+CD11b+ dendritic cells in utero and allergic inflammation in neonates.

American journal of physiology. Lung cellular and molecular physiology

Abdala-Valencia H,Soveg F,Cook-Mills JM

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

Batf3 maintains autoactivation of Irf8 for commitment of a CD8α(+) conventional DC clonogenic progenitor.

Nature immunology

Grajales-Reyes GE,Iwata A,Albring J,Wu X,Tussiwand R,Kc W,Kretzer NM,Briseño CG,Durai V,Bagadia P,Haldar M,Schönheit J,Rosenbauer F,Murphy TL,Murphy KM

12-9858 was used in Flow cytometry/Cell sorting to investigate the role of the transcription factors Batf3 and IRF8 in the development of CD8α(+) conventional dendritic cells (cDCs)..

Wed Jul 01 00:00:00 EDT 2015

Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.

Journal of immunology (Baltimore, Md. : 1950)

Nündel K,Green NM,Shaffer AL,Moody KL,Busto P,Eilat D,Miyake K,Oropallo MA,Cancro MP,Marshak-Rothstein A

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Beta-catenin signaling drives differentiation and proinflammatory function of IRF8-dependent dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cohen SB,Smith NL,McDougal C,Pepper M,Shah S,Yap GS,Acha-Orbea H,Jiang A,Clausen BE,Rudd BD,Denkers EY

Published figure using IRF4 monoclonal antibody (Product # 12-9858-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP

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Gene Aliases: AI385587; IRF-4; IRF4; LSIRF; MUM1; NF-EM5; SHEP8; Spip

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UniProt ID: (Human) Q15306, (Mouse) Q64287

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Entrez Gene ID: (Human) 3662, (Mouse) 16364

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein-lysine N-methyltransferase activity sequence-specific DNA binding regulatory region DNA binding DNA binding winged helix/forkhead transcription factor

Process(es)

transcription from RNA polymerase II promoter peptidyl-lysine methylation negative regulation of toll-like receptor signaling pathway T cell activation defense response to protozoan myeloid dendritic cell differentiation positive regulation of DNA binding histone H3 acetylation histone H4 acetylation positive regulation of interleukin-10 biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-4 biosynthetic process regulation of T-helper cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter interferon-gamma-mediated signaling pathway type I interferon signaling pathway T-helper 17 cell lineage commitment transcription, DNA-templated regulation of transcription, DNA-templated protein methylation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRF4 Monoclonal Antibody (3E4), PE, eBioscience™

IRF4 Antibody (12-9858-82) in Flow

Product Details

12-9858-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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