Invitrogen

INSR alpha Monoclonal Antibody (83-14)

Advanced Verification

IP-mass spectrometry

5 References
View all (15) INSR alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-13759

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:10-1:100
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

2 µg/mL
View 3 publications 3 publications

Neutralization (Neu)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

83-14

Immunogen

IM-9 lymphocytes followed by purified insulin receptor
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985953

Product Specific Information

MA5-13759 targets Insulin Receptor alpha in WB, ICC/IF, ELISA and IP applications and shows reactivity with Human samples.

The MA5-13759 immunogen is iM-9 lymphocytes followed by purified insulin receptor.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD220; CD220 beta; Insulin receptor; IR; IR alpha; IR beta

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Gene Aliases: CD220; HHF5; INSR

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UniProt ID: (Human) P06213

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Entrez Gene ID: (Human) 3643

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Function(s)
protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding protein binding ATP binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor I binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding PTB domain binding transmembrane signal receptor
Process(es)
activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis carbohydrate metabolic process regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway epidermis development male gonad development response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation male sex determination adrenal gland development positive regulation of cell migration exocrine pancreas development activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity cellular response to insulin stimulus response to vitamin D response to testosterone response to tumor necrosis factor peptidyl-tyrosine autophosphorylation glucose homeostasis positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process fat cell differentiation response to ethanol positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import protein autophosphorylation positive regulation of developmental growth protein heterotetramerization response to glucocorticoid positive regulation of meiotic cell cycle positive regulation of protein kinase B signaling positive regulation of respiratory burst cellular response to growth factor stimulus regulation of female gonad development
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