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Invitrogen
IL-17AF Monoclonal Antibody (20LJS09), eFluor™ 450, eBioscience™
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IL-17AF Antibody (48-9179-42) in Flow
Product Details
48-9179-42
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Product Specific Information
Description: The 20LJS09 monoclonal antibody reacts with human interleukin (IL)-17AF heterodimer. This antibody specifically reacts with this heterodimer and has no reactivity for IL-17A or IL-17F homodimers. IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.
Applications Reported: This 20LJS09 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 20LJS09 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of in vitro-polarized human Th17 cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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References (0)
Bioinformatics
Protein Aliases: CTLA-8; Cytokine ML-1; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; IL-17F; ILN; Interleukin; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin-17F
Gene Aliases: CANDF6; CTLA-8; CTLA8; IL-17; IL-17A; IL-17F; IL17; IL17A; IL17F; ML-1; ML1
UniProt ID: (Human) Q16552, (Human) Q96PD4
Entrez Gene ID: (Human) 3605, (Human) 112744
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