Invitrogen

IL-17A Monoclonal Antibody (eBio17B7), eFluor™ 506, eBioscience™

IL-17A Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 97

IL-17A Antibody (69-7177-82) in Flow

Th17-polarized mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (Product # 00-4975-03) (right) and then intracellularly stained with Anti-Mouse CD4 APC (Product # 17-0042-82) and 0.25 µg of Anti-Mouse/Rat IL-17A eFluor® 506, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

IL-17A Antibody in Immunohistochemistry (IHC)

IL-17A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IL-17A Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

IL-17A Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

69-7177-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 75 publications 75 publications

ELISA (ELISA)

View 3 publications 3 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 506, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio17B7

Conjugate

eFluor™ 506 eFluor™ 506 eFluor™ 506

Excitation/Emission Max

419/508 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637303

Product Specific Information

Description: The eBio17B7 antibody reacts with mouse and rat IL-17A with no recognition of IL-17F. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13, an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Applications Reported: This eBio17B7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio17B7 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Intracellular staining with eBio17B7 may also be done using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online.

eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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International journal of molecular sciences

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Scientific reports

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EMBO reports

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MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

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Thu Feb 01 00:00:00 EST 2018

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Experimental and therapeutic medicine

Liu Y,Sun L,Chen W,Chuai J,Shang Y,Zhang D,Fu B,Tian H,Jiang S

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Thu Feb 01 00:00:00 EST 2018

Baicalin Downregulates RLRs Signaling Pathway to Control Influenza A Virus Infection and Improve the Prognosis.

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Pang P,Zheng K,Wu S,Xu H,Deng L,Shi Y,Chen X

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eLife

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Vibrio vulnificus RtxA Is a Major Factor Driving Inflammatory T Helper Type 17 Cell Responses in vitro and in vivo.

Frontiers in immunology

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ITGB4 deficiency in bronchial epithelial cells directs airway inflammation and bipolar disorder-related behavior.

Journal of neuroinflammation

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Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation.

Nature communications

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JCI insight

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Nature

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miR-146a modulates autoreactive Th17 cell differentiation and regulates organ-specific autoimmunity.

The Journal of clinical investigation

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Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

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Critical role of caspase-8-mediated IL-1 signaling in promoting Th2 responses during asthma pathogenesis.

Mucosal immunology

Qi X,Gurung P,Malireddi RK,Karmaus PW,Sharma D,Vogel P,Chi H,Green DR,Kanneganti TD

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PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

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Wed Feb 15 00:00:00 EST 2017

PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Webb LM,Amici SA,Jablonski KA,Savardekar H,Panfil AR,Li L,Zhou W,Peine K,Karkhanis V,Bachelder EM,Ainslie KM,Green PL,Li C,Baiocchi RA,Guerau-de-Arellano M

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Placenta‑derived mesenchymal stem cells improve airway hyperresponsiveness and inflammation in asthmatic rats by modulating the Th17/Treg balance.

Molecular medicine reports

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Fri Dec 01 00:00:00 EST 2017

Upregulation of SOCS3 in lung CD4+ T cells in a mouse model of chronic PA lung infection and suppression of Th17‑mediated neutrophil recruitment in exogenous SOCS3 transfer in vitro.

Molecular medicine reports

Ding FM,Liao RM,Chen YQ,Xie GG,Zhang PY,Shao P,Zhang M

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation.

Scientific reports

Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2017

Microsomal Prostaglandin E Synthase-1 Facilitates an Intercellular Interaction between CD4⁺ T Cells through IL-1β Autocrine Function in Experimental Autoimmune Encephalomyelitis.

International journal of molecular sciences

Takemiya T,Takeuchi C,Kawakami M

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Immunofluorescence

Tue Dec 19 00:00:00 EST 2017

Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice.

JCI insight

Robert R,Ang C,Sun G,Juglair L,Lim EX,Mason LJ,Payne NL,Bernard CC,Mackay CR

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells.

Frontiers in pharmacology

Li M,Gao J,Tang Y,Liu M,Wu S,Qu K,Long X,Li H,Liu M,Liu Y,Yuan J,Mao L,Liu Y,Zheng X,Wang E,Wang J,Yang Y

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome.

Cell

Chudnovskiy A,Mortha A,Kana V,Kennard A,Ramirez JD,Rahman A,Remark R,Mogno I,Ng R,Gnjatic S,Amir ED,Solovyov A,Greenbaum B,Clemente J,Faith J,Belkaid Y,Grigg ME,Merad M

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Thu Oct 06 00:00:00 EDT 2016

Oligodendrocyte death results in immune-mediated CNS demyelination.

Nature neuroscience

Traka M,Podojil JR,McCarthy DP,Miller SD,Popko B

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Nuclear receptor expression atlas in BMDCs: Nr4a2 restricts immunogenicity of BMDCs and impedes EAE.

European journal of immunology

Saini A,Mahajan S,Gupta P

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

p(⁷⁰S⁶K¹) in the TORC1 pathway is essential for the differentiation of Th17 Cells, but not Th1, Th2, or Treg cells in mice.

European journal of immunology

Sasaki CY,Chen G,Munk R,Eitan E,Martindale J,Longo DL,Ghosh P

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Rapamycin ameliorates CCl4-induced liver fibrosis in mice through reciprocal regulation of the Th17/Treg cell balance.

Molecular medicine reports

Gu L,Deng WS,Sun XF,Zhou H,Xu Q

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Age-dependent divergent effects of OX40L treatment on the development of diabetes in NOD mice.

Autoimmunity

Haddad CS,Bhattacharya P,Alharshawi K,Marinelarena A,Kumar P,El-Sayed O,Elshabrawy HA,Epstein AL,Prabhakar BS

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Tropomyosin Receptor Kinase C Targeted Delivery of a Peptidomimetic Ligand-Photosensitizer Conjugate Induces Antitumor Immune Responses Following Photodynamic Therapy.

Scientific reports

Kue CS,Kamkaew A,Voon SH,Kiew LV,Chung LY,Burgess K,Lee HB

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Thu Nov 17 00:00:00 EST 2016

Tamm-Horsfall Protein Regulates Granulopoiesis and Systemic Neutrophil Homeostasis.

Journal of the American Society of Nephrology : JASN

Micanovic R,Chitteti BR,Dagher PC,Srour EF,Khan S,Hato T,Lyle A,Tong Y,Wu XR,El-Achkar TM

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2015

Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Li JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.

Stem cell reports

Wang WB,Yen ML,Liu KJ,Hsu PJ,Lin MH,Chen PM,Sudhir PR,Chen CH,Chen CH,Sytwu HK,Yen BL

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with Staphylococcus aureus.

PloS one

Wang XY,Huang ZX,Chen YG,Lu X,Zhu P,Wen K,Fu N,Liu BY

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Tue May 24 00:00:00 EDT 2016

Upregulation of Programmed Death-1 and Its Ligand in Cardiac Injury Models: Interaction with GADD153.

PloS one

Baban B,Liu JY,Qin X,Weintraub NL,Mozaffari MS

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Tue Apr 12 00:00:00 EDT 2016

A chitin-like component on sclerotic cells of Fonsecaea pedrosoi inhibits Dectin-1-mediated murine Th17 development by masking β-glucans.

PloS one

Dong B,Li D,Li R,Chen SC,Liu W,Liu W,Chen L,Chen Y,Zhang X,Tong Z,Xia Y,Xia P,Wang Y,Duan Y

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2015

R-phycoerythrin-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Kim MS,Kim TS

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Interleukin-22 as a molecular adjuvant facilitates IL-17-producing CD8+ T cell responses against a HBV DNA vaccine in mice.

Human vaccines & immunotherapeutics

Wu B,Zou Q,Hu Y,Wang B

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Prostaglandin E2 suppresses antifungal immunity by inhibiting interferon regulatory factor 4 function and interleukin-17 expression in T cells.

Immunity

Valdez PA,Vithayathil PJ,Janelsins BM,Shaffer AL,Williamson PR,Datta SK

69-7177 was used in Flow cytometry/Cell sorting to study the effect of prostaglandin E2 on the function of Th17 cells during differentiation.

Fri Apr 20 00:00:00 EDT 2012

Soluble MOG35-55/I-A(b) dimers ameliorate experimental autoimmune encephalomyelitis by reducing encephalitogenic T cells.

PloS one

Gong Y,Wang Z,Liang Z,Duan H,Ouyang L,Yu Q,Xu Z,Shen G,Weng X,Wu X

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Fri Jul 19 00:00:00 EDT 2013

Anti-IL-23 antibody blockade of IL-23/IL-17 pathway attenuates airway obliteration in rat orthotopic tracheal transplantation.

International immunopharmacology

Cao H,Lan Q,Shi Q,Zhou X,Liu G,Liu J,Tang G,Qiu C,Qiu C,Xu J,Fan H,Liu Z

Sun May 01 00:00:00 EDT 2011

Role of epithelial integrin-linked kinase in promoting intestinal inflammation: effects on CCL2, fibronectin and the T cell repertoire.

BMC immunology

Assi K,Patterson S,Dedhar S,Owen D,Levings M,Salh B

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2011

T cells from Programmed Death-1 deficient mice respond poorly to Mycobacterium tuberculosis infection.

PloS one

Tousif S,Singh Y,Prasad DV,Sharma P,Van Kaer L,Das G

Thu May 12 00:00:00 EDT 2011

Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Ellestad KK,Tsutsui S,Noorbakhsh F,Warren KG,Yong VW,Pittman QJ,Power C

Wed Jul 01 00:00:00 EDT 2009

IDO activates regulatory T cells and blocks their conversion into Th17-like T cells.

Journal of immunology (Baltimore, Md. : 1950)

Baban B,Chandler PR,Sharma MD,Pihkala J,Koni PA,Munn DH,Mellor AL

Published figure using IL-17A monoclonal antibody (Product # 69-7177-82) in Flow Cytometry

Sat Aug 15 00:00:00 EDT 2009

Adult-like anti-mycobacterial T cell and in vivo dendritic cell responses following neonatal immunization with Ag85B-ESAT-6 in the IC31 adjuvant.

PloS one

Kamath AT,Rochat AF,Valenti MP,Agger EM,Lingnau K,Andersen P,Lambert PH,Siegrist CA

69-7177 was used in Flow cytometry/Cell sorting to assess the immunogenicity of a novel candidate vaccine comprising a mycobacterial fusion protein, Ag85B-ESAT-6, in a neonatal murine immunization model.

Tue Dec 23 00:00:00 EST 2008

Bioinformatics

Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A

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Gene Aliases: CTLA-8; Ctla8; IL-17; IL-17A; Il17; Il17a

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UniProt ID: (Mouse) Q62386

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Entrez Gene ID: (Mouse) 16171, (Rat) 301289

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Function(s)

cytokine activity

Process(es)

inflammatory response positive regulation of necrotic cell death positive regulation of interleukin-23 production positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to interleukin-1 cellular response to glucocorticoid stimulus fibroblast activation positive regulation of cytokine production involved in inflammatory response response to amino acid

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IL-17A Monoclonal Antibody (eBio17B7), eFluor™ 506, eBioscience™

IL-17A Antibody (69-7177-82) in Flow

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69-7177-82

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