Invitrogen

IL-13 Monoclonal Antibody (eBio13A), eFluor™ 450, eBioscience™

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IL-13 Antibody (48-7133-82) in Flow

BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), Mouse IL-2 Recombinant Protein (Product # 14-8021-64), and Mouse IL-4 Recombinant Protein (Product # 14-8041-80). Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized and then stained with Anti-Mouse CD4 FITC (Product # 11-004... View More

IL-13 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

IL-13 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

48-7133-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 32 publications 32 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio13A

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11219690

Product Specific Information

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2-polarized mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Self-amplifying mRNA seasonal influenza vaccines elicit mouse neutralizing antibody and cell-mediated immunity and protect ferrets.

NPJ vaccines

Cheung M,Chang C,Rathnasinghe R,Rossignol E,Zhang Y,Ferrari A,Patel H,Huang Y,Sanchez Guillen M,Scalzo T,Lee C,Otten GR,Settembre EC,Music N,Palladino G,Wen Y

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Wed Oct 04 00:00:00 EDT 2023

Monitoring Group 2 Innate Lymphoid Cell Biology in Models of Lung Inflammation.

Bio-protocol

Badrani JH,Strohm AN,Haung YA,Doherty TA

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Thu Jul 20 00:00:00 EDT 2023

Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.

Nature communications

Cheong LY,Wang B,Wang Q,Jin L,Kwok KHM,Wu X,Shu L,Lin H,Chung SK,Cheng KKY,Hoo RLC,Xu A

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

SLAMF1 Is Dispensable for Vaccine-Induced T Cell Development but Required for Resistance to Fungal Infection.

Journal of immunology (Baltimore, Md. : 1950)

Kohn EM,Dos Santos Dias L,Dobson HE,He X,Wang H,Klein BS,Wüthrich M

48-7133-82 was used in Flow cytometry/Cell sorting to report that SLAMF1 is indispensable for host resistance to primary and vaccine-induced protection against fungal infection.

Tue Mar 15 00:00:00 EDT 2022

Mitochondrial transcription factor A in RORγt⁺ lymphocytes regulate small intestine homeostasis and metabolism.

Nature communications

Fu Z,Dean JW,Xiong L,Dougherty MW,Oliff KN,Chen ZE,Jobin C,Garrett TJ,Zhou L

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Thu Jul 22 00:00:00 EDT 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in ELISA

Fri Oct 22 00:00:00 EDT 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in ELISA

Fri Oct 22 00:00:00 EDT 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

6-Phosphogluconate dehydrogenase (6PGD), a key checkpoint in reprogramming of regulatory T cells metabolism and function.

eLife

Daneshmandi S,Cassel T,Higashi RM,Fan TW,Seth P

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Thu Oct 28 00:00:00 EDT 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2.

iScience

Park JG,Oladunni FS,Rohaim MA,Whittingham-Dowd J,Tollitt J,Hodges MDJ,Fathallah N,Assas MB,Alhazmi W,Almilaibary A,Iqbal M,Chang P,Escalona R,Shivanna V,Torrelles JB,Worthington JJ,Jackson-Jones LH,Martinez-Sobrido L,Munir M

48-7133-82 was used in Flow Cytometry to evaluate a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy against SARS-CoV-2.

Fri Sep 24 00:00:00 EDT 2021

A helminth-derived suppressor of ST2 blocks allergic responses.

eLife

Vacca F,Chauché C,Jamwal A,Hinchy EC,Heieis G,Webster H,Ogunkanbi A,Sekne Z,Gregory WF,Wear M,Perona-Wright G,Higgins MK,Nys JA,Cohen ES,McSorley HJ

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice.

Nature communications

Wang G,Xu J,Zhao J,Yin W,Liu D,Chen W,Hou SX

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation.

Cell reports

Jaeger N,McDonough RT,Rosen AL,Hernandez-Leyva A,Wilson NG,Lint MA,Russler-Germain EV,Chai JN,Bacharier LB,Hsieh CS,Kau AL

48-7133 was used in Flow cytometry/Cell sorting to investigate how chronic colonization impacts mucosal immunity and the development of allergic airway inflammation (AAI).

Tue Nov 03 00:00:00 EST 2020

CD62L Is a Functional and Phenotypic Marker for Circulating Innate Lymphoid Cell Precursors.

Journal of immunology (Baltimore, Md. : 1950)

Bar-Ephraim YE,Koning JJ,Burniol Ruiz E,Konijn T,Mourits VP,Lakeman KA,Boon L,Bögels M,van Maanen JP,Den Haan JMM,van Egmond M,Bouma G,Reijmers RM,Mebius RE

48-7133-82 was used in Flow Cytometry to demonstrate that CD62L not only serves as a functional marker of ILCP, but has potential to be used in the clinic as a diagnostic marker in inflammatory disorders.

Tue Jan 01 00:00:00 EST 2019

Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes.

Nature communications

Galle-Treger L,Sankaranarayanan I,Hurrell BP,Howard E,Lo R,Maazi H,Lewis G,Banie H,Epstein AL,Hu P,Rehan VK,Gilliland FD,Allayee H,Soroosh P,Sharpe AH,Akbari O

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Feb 12 00:00:00 EST 2019

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

48-7133 was used in Flow cytometry/Cell sorting to investigate the role of TNF/TNFR2 axis in pulmonary ILC2s for possible use in treating ILC2-dependent asthma, in murine and human subjects.

Tue Dec 24 00:00:00 EST 2019

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

48-7133 was used in Flow cytometry/Cell sorting to investigate the role of TNF/TNFR2 axis in pulmonary ILC2s for possible use in treating ILC2-dependent asthma, in murine and human subjects.

Tue Dec 24 00:00:00 EST 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Autoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis.

Immunity

Hirota K,Hashimoto M,Ito Y,Matsuura M,Ito H,Tanaka M,Watanabe H,Kondoh G,Tanaka A,Yasuda K,Kopf M,Potocnik AJ,Stockinger B,Sakaguchi N,Sakaguchi S

48-7133 was used in Flow cytometry/Cell sorting to study the signalling pathway responsible for mediating the stimulation of fibroblast-like synoviocytes by arthritogenic Th17 cells.

Tue Jun 19 00:00:00 EDT 2018

A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.

Immunity

Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM

48-7133-82 was used in Flow Cytometry to point to an M-CSF-producing γδ T cell subset that fulfills a specialized protective role in the later stage of malaria infection when αβ T cells have declined.

Tue Feb 20 00:00:00 EST 2018

Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells.

Arthritis research & therapy

Saigusa R,Asano Y,Taniguchi T,Hirabayashi M,Nakamura K,Miura S,Yamashita T,Takahashi T,Ichimura Y,Toyama T,Yoshizaki A,Trojanowska M,Sato S

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Wed Feb 07 00:00:00 EST 2018

Nematode-Infected Mice Acquire Resistance to Subsequent Infection With Unrelated Nematode by Inducing Highly Responsive Group 2 Innate Lymphoid Cells in the Lung.

Frontiers in immunology

Yasuda K,Adachi T,Koida A,Nakanishi K

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.

Genome medicine

Pramanik J,Chen X,Kar G,Henriksson J,Gomes T,Park JE,Natarajan K,Meyer KB,Miao Z,McKenzie ANJ,Mahata B,Teichmann SA

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

Group 2 innate lymphoid cells protect lung endothelial cells from pyroptosis in sepsis.

Cell death & disease

Lai D,Tang J,Chen L,Fan EK,Scott MJ,Li Y,Billiar TR,Wilson MA,Fang X,Shu Q,Fan J

48-7133 was used in Flow cytometry/Cell sorting to describe the function of ILC2 in the regulation of lung inflammation by protecting lung endothelial cell from pyroptosis.

Tue Mar 06 00:00:00 EST 2018

Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.

Immunity

Minutti CM,Drube S,Blair N,Schwartz C,McCrae JC,McKenzie AN,Kamradt T,Mokry M,Coffer PJ,Sibilia M,Sijts AJ,Fallon PG,Maizels RM,Zaiss DM

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.

Immunity

Osbourn M,Soares DC,Vacca F,Cohen ES,Scott IC,Gregory WF,Smyth DJ,Toivakka M,Kemter AM,le Bihan T,Wear M,Hoving D,Filbey KJ,Hewitson JP,Henderson H,Gonzàlez-Cìscar A,Errington C,Vermeren S,Astier AL,Wallace WA,Schwarze J,Ivens AC,Maizels RM,McSorley HJ

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region.

PloS one

Kim K,Kim N,Lee GR

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Thu Jul 07 00:00:00 EDT 2016

Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection.

PLoS pathogens

Wiesner DL,Specht CA,Lee CK,Smith KD,Mukaremera L,Lee ST,Lee CG,Elias JA,Nielsen JN,Boulware DR,Bohjanen PR,Jenkins MK,Levitz SM,Nielsen K

48-7133 was used in Flow cytometry/Cell sorting to investigate Th2 cell responses to pulmonary fungal infection, showing that chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection.

Sun Mar 01 00:00:00 EST 2015

The Alarmin HMGN1 contributes to antitumor immunity and is a potent immunoadjuvant.

Cancer research

Wei F,Yang D,Tewary P,Li Y,Li S,Chen X,Howard OM,Bustin M,Oppenheim JJ

48-7133 was used in Flow cytometry/Cell sorting to demonstrate the alarmin HMGN1 contributes to antitumor immunity and may offer an effective adjuvant to heighten responses to cancer vaccines.

Sat Nov 01 00:00:00 EDT 2014

Type 2 innate lymphoid cells control eosinophil homeostasis.

Nature

Nussbaum JC,Van Dyken SJ,von Moltke J,Cheng LE,Mohapatra A,Molofsky AB,Thornton EE,Krummel MF,Chawla A,Liang HE,Locksley RM

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Flow Cytometry

Thu Oct 10 00:00:00 EDT 2013

Bacterial colonization dampens influenza-mediated acute lung injury via induction of M2 alveolar macrophages.

Nature communications

Wang J,Li F,Sun R,Gao X,Wei H,Li LJ,Tian Z

Published figure using IL-13 monoclonal antibody (Product # 48-7133-82) in Neutralization

Mon Dec 30 00:00:00 EST 2013

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

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Gene Aliases: Il-13; Il13

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UniProt ID: (Mouse) P20109

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Entrez Gene ID: (Mouse) 16163

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Function(s)

cytokine activity cytokine receptor binding interleukin-13 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-13 Monoclonal Antibody (eBio13A), eFluor™ 450, eBioscience™

IL-13 Antibody (48-7133-82) in Flow

Product Details

48-7133-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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