Invitrogen

IBA1 Polyclonal Antibody

IBA1 Antibody in Immunocytochemistry (ICC/IF)

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IBA1 Antibody (PA5-18519) in ICC/IF

Immunocytochemistry analysis of IBA1 using IBA1 Polyclonal Antibody (Product # PA5-18519) in paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-18519

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

10 µg/mL

Flow Cytometry (Flow)

10 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (YEEKAREKEKP) corresponding to the C-terminus amino acids of AIF1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10980175

Product Specific Information

This antibody is predicted to react with canine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Target Information

AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fgr contributes to hemorrhage-induced thalamic pain by activating NF-κB/ERK1/2 pathways.

JCI insight

Huang T,Fu G,Gao J,Zhang Y,Cai W,Wu S,Jia S,Xia S,Bachmann T,Bekker A,Tao YX

PA5-18519 was used in Immunohistochemistry (PFA fixed) to suggest that Fgr is a key player in thalamic pain and a potential target for the therapeutic management of this disorder.

Thu Oct 15 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: AIF-1; Allograft inflammatory factor 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; Protein G1

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Gene Aliases: AIF-1; AIF1; G1; IBA1; IRT-1; IRT1

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UniProt ID: (Human) P55008

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Entrez Gene ID: (Human) 199

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Function(s)

molecular_function calcium ion binding actin filament binding calmodulin-related

Process(es)

microglial cell activation positive regulation of protein phosphorylation phagocytosis, engulfment inflammatory response negative regulation of gene expression positive regulation of muscle hyperplasia Rac protein signal transduction actin filament polymerization cellular response to extracellular stimulus cellular response to hormone stimulus positive regulation of T cell proliferation negative regulation of apoptotic process positive regulation of nitric oxide biosynthetic process positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation response to axon injury actin filament bundle assembly response to glucocorticoid response to electrical stimulus cellular response to morphine cellular response to interferon-gamma cellular response to hydroperoxide negative regulation of smooth muscle cell chemotaxis positive regulation of smooth muscle cell chemotaxis positive regulation of monocyte chemotaxis ruffle assembly positive regulation of G1/S transition of mitotic cell cycle positive regulation of T cell migration

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IBA1 Polyclonal Antibody

IBA1 Antibody (PA5-18519) in ICC/IF

Product Details

PA5-18519

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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