Invitrogen

HSP27 Polyclonal Antibody

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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FIGURE: 1 / 5

HSP27 Antibody (AHO1132) in WB

Western blot was performed using Anti-HSP27 Polyclonal Antibody(Product # AHO1132) and a 25kDa band corresponding to HSP27 was observed across cell lines tested except HT-1080 which is reported to be negative. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), PC-3 (Lane 3), DU 145 (Lane 4), HeLa (Lane 5), LNCaP (Lane 6) and HT-1080 (Lane 7) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by i... View More

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HSP27 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of HSP27 was observed in MCF-7, MDA-MB-231, PC-3, DU 145, HeLa and LNCaP in comparison to HT-1080 using Anti-HSP27 Polyclonal Antibody (Product # AHO1132) in Western Blot. {RE}

Product Details

AHO1132

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full length human HSP27 expressed in E. coli.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536323

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; heat shock protein, 25 kDa; HS; HSP; HSP 25; HSP 27; HspB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25

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Gene Aliases: 27kDa; CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27

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UniProt ID: (Human) P04792, (Mouse) P14602

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Entrez Gene ID: (Human) 3315, (Mouse) 15507, (Rat) 24471

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Function(s)

protein kinase C binding protein binding protein kinase C inhibitor activity protein kinase binding identical protein binding ubiquitin binding poly(A) RNA binding

Process(es)

negative regulation of protein kinase activity response to unfolded protein positive regulation of interleukin-1 beta production intracellular signal transduction cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of tumor necrosis factor biosynthetic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis platelet aggregation negative regulation of protein serine/threonine kinase activity negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of endothelial cell chemotaxis negative regulation of apoptotic signaling pathway retina homeostasis regulation of translational initiation movement of cell or subcellular component response to virus regulation of autophagy negative regulation of apoptotic process regulation of mRNA stability vascular endothelial growth factor receptor signaling pathway

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HSP27 Polyclonal Antibody

HSP27 Antibody (AHO1132) in WB

Product Details

AHO1132

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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