Invitrogen

HLA-DR Monoclonal Antibody (LN3), Alexa Fluor™ 514

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

753-9956-82

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

LN3

Conjugate

Alexa Fluor™ 514 Alexa Fluor™ 514 Alexa Fluor™ 514

Excitation/Emission Max

518/543 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

Product Specific Information

Description: The LN3 antibody reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.

Applications Reported: The LN3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Immunohistochemistry can be performed using frozen and Bouin's, or formalin/paraffin, human tissues.

Applications Tested: This LN3 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DASS-397D15.1; DR beta-5; DR-9; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; leukocyte antigen; leukocyte antigen class II; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen HLA-DR-beta; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; p33; XXbac-BPG161M6.1

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Gene Aliases: CD74; DHLAG; DR-4; DR4; DRB1; DRB4; DRw10; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; Ia-GAMMA; II; MLRW; SS1

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UniProt ID: (Human) P04233, (Human) P01903, (Human) Q5UBA2, (Human) P79483, (Human) P13762, (Human) Q30154

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Entrez Gene ID: (Human) 972, (Human) 3122, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127

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Function(s)
beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding MHC class II receptor activity peptide antigen binding major histocompatibility complex protein scaffold/adaptor protein
Process(es)
activation of MAPK activity prostaglandin biosynthetic process positive regulation of cytokine-mediated signaling pathway positive regulation of dendritic cell antigen processing and presentation negative regulation of peptide secretion positive regulation of type 2 immune response negative regulation of mature B cell apoptotic process protein complex assembly intracellular protein transport defense response signal transduction cell proliferation immunoglobulin mediated immune response antigen processing and presentation of endogenous antigen antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of B cell proliferation macrophage migration inhibitory factor signaling pathway regulation of macrophage activation negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator T cell selection positive thymic T cell selection negative thymic T cell selection negative regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration chaperone mediated protein folding requiring cofactor positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production peptide antigen assembly with MHC class II protein complex antigen processing and presentation of peptide or polysaccharide antigen via MHC class II polysaccharide assembly with MHC class II protein complex immune response viral process T cell costimulation T cell receptor signaling pathway cognition interferon-gamma-mediated signaling pathway
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