Invitrogen

HLA-DR Monoclonal Antibody (LN3), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

25 Published Figures

25 References

View all (121) HLA-DR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HLA-DR Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 27

HLA-DR Antibody (25-9956-42) in Flow

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human HLA-DR PE-Cyanine7 (filled histogram). Cells in the lymphocyte (right) or monocyte (left) gate were used for analysis.

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Product Details

25-9956-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.015 µg)/test

View 25 publications 25 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

LN3

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582284

Product Specific Information

Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.

Applications Reported: This LN3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Activated CD4⁺ T cells and CD14^hiCD16⁺ monocytes correlate with antibody response following influenza virus infection in humans.

Cell reports. Medicine

Wong SS,Oshansky CM,Guo XJ,Ralston J,Wood T,Reynolds GE,Seeds R,Jelley L,Waite B,Jeevan T,Zanin M,Widdowson MA,Huang QS,Thomas PG,Webby RJ

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Tue Apr 20 00:00:00 EDT 2021

The role of irreversible electroporation in promoting M1 macrophage polarization via regulating the HMGB1-RAGE-MAPK axis in pancreatic cancer.

Oncoimmunology

He C,Sun S,Zhang Y,Xie F,Li S

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Thu Mar 11 00:00:00 EST 2021

Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model.

Stem cell research & therapy

Fan Y,Sun J,Zhang Q,Lai D

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Wed Mar 24 00:00:00 EDT 2021

GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure.

Cell death & disease

Yu H,Yang W,Huang J,Miao X,Wang B,Ren X,Gu Y,Wang Q,Ding X,Guo X,Qian F,Zhang Y,Xu H,Zheng L,Jin M

25-9956-42 was used in Flow cytometry/Cell sorting to investigate whether GPR120, the receptor of polyunsaturated long chain fatty acids, is involved in fulminant hepatic failure (FHF).

Thu Dec 16 00:00:00 EST 2021

Lung transplantation for patients with severe COVID-19.

Science translational medicine

Bharat A,Querrey M,Markov NS,Kim S,Kurihara C,Garza-Castillon R,Manerikar A,Shilatifard A,Tomic R,Politanska Y,Abdala-Valencia H,Yeldandi AV,Lomasney JW,Misharin AV,Budinger GRS

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation.

PLoS pathogens

Oliveira AER,Pereira MCA,Belew AT,Ferreira LRP,Pereira LMN,Neves EGA,Nunes MDCP,Burleigh BA,Dutra WO,El-Sayed NM,Gazzinelli RT,Teixeira SMR

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Characterization of HLA-G Regulation and HLA Expression in Breast Cancer and Malignant Melanoma Cell Lines upon IFN-γ Stimulation and Inhibition of DNA Methylation.

International journal of molecular sciences

Jørgensen N,Sayed A,Jeppesen HB,Persson G,Weisdorf I,Funck T,Hviid TVF

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Wed Jun 17 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

New GMP manufacturing processes to obtain thermostable HIV-1 gp41 virosomes under solid forms for various mucosal vaccination routes.

NPJ vaccines

Amacker M,Smardon C,Mason L,Sorrell J,Jeffery K,Adler M,Bhoelan F,Belova O,Spengler M,Punnamoottil B,Schwaller M,Bonduelle O,Combadière B,Stegmann T,Naylor A,Johnson R,Wong D,Fleury S

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantation.

Nature communications

Esfahani K,Al-Aubodah TA,Thebault P,Lapointe R,Hudson M,Johnson NA,Baran D,Bhulaiga N,Takano T,Cailhier JF,Piccirillo CA,Miller WH

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Thu Oct 17 00:00:00 EDT 2019

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

Large-Scale Human Dendritic Cell Differentiation Revealing Notch-Dependent Lineage Bifurcation and Heterogeneity.

Cell reports

Balan S,Arnold-Schrauf C,Abbas A,Couespel N,Savoret J,Imperatore F,Villani AC,Vu Manh TP,Bhardwaj N,Dalod M

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Tue Aug 14 00:00:00 EDT 2018

Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells.

Stem cells (Dayton, Ohio)

Sontag S,Förster M,Qin J,Wanek P,Mitzka S,Schüler HM,Koschmieder S,Rose-John S,Seré K,Zenke M

Sat Apr 01 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Gut-homing Δ42PD1⁺Vδ2 T cells promote innate mucosal damage via TLR4 during acute HIV type 1 infection.

Nature microbiology

Cheung AKL,Kwok HY,Huang Y,Chen M,Mo Y,Wu X,Lam KS,Kong HK,Lau TCK,Zhou J,Li J,Cheng L,Kiat Lee B,Peng Q,Lu X,An M,Wang H,Shang H,Zhou B,Wu H,Xu A,Yuen KY,Chen Z

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

FOXP1 suppresses immune response signatures and MHC class II expression in activated B-cell-like diffuse large B-cell lymphomas.

Leukemia

Brown PJ,Wong KK,Felce SL,Lyne L,Spearman H,Soilleux EJ,Pedersen LM,Møller MB,Green TM,Gascoyne DM,Banham AH

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using HLA-DR monoclonal antibody (Product # 25-9956-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Cellular immune activation in cerebrospinal fluid from ugandans with cryptococcal meningitis and immune reconstitution inflammatory syndrome.

The Journal of infectious diseases

Meya DB,Okurut S,Zziwa G,Rolfes MA,Kelsey M,Cose S,Joloba M,Naluyima P,Palmer BE,Kambugu A,Mayanja-Kizza H,Bohjanen PR,Eller MA,Wahl SM,Boulware DR,Manabe YC,Janoff EN

25-9956 was used in Flow cytometry/Cell sorting to study the immunopathogenesis of cryptococcal meningitis immune reconstitution inflammatory syndrome following initiation of antiretroviral therapy at the site of infection.

Fri May 15 00:00:00 EDT 2015

Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.

Human immunology

Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA

25-9956 was used in Flow cytometry/Cell sorting to show that relative to term, prematurity and chorioamnionitis decrease Treg cell functionality without affecting their frequency or phenotype.

Thu Jan 01 00:00:00 EST 2015

Multiparametric bioinformatics distinguish the CD4/CD8 ratio as a suitable laboratory predictor of combined T cell pathogenesis in HIV infection.

Journal of immunology (Baltimore, Md. : 1950)

Buggert M,Frederiksen J,Noyan K,Svärd J,Barqasho B,Sönnerborg A,Lund O,Nowak P,Karlsson AC

25-9956 was used in Flow cytometry/Cell sorting to investigate how the CD4/CD8 ratio would be a suitable laboratory predictor in future clinical and therapeutic settings to monitor pathological T cell events in HIV infection.

Sat Mar 01 00:00:00 EST 2014

Humanized mice, a new model to study the influence of drug treatment on neonatal sepsis.

Infection and immunity

Ernst W,Zimara N,Hanses F,Männel DN,Seelbach-Göbel B,Wege AK

25-9956 was used in Flow cytometry/Cell sorting to investigate the use of humanised mice as a model of GBS-induced sepsis.

Wed May 01 00:00:00 EDT 2013

Leishmania major infection in humanized mice induces systemic infection and provokes a nonprotective human immune response.

PLoS neglected tropical diseases

Wege AK,Florian C,Ernst W,Zimara N,Schleicher U,Hanses F,Schmid M,Ritter U

25-9956 was used in Flow cytometry/Cell sorting to describe Leishmania major infection in humanised mice for the first time, characterising disease development and the induction of the human immune response.

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DASS-397D15.1; DR beta-5; DR-9; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; leukocyte antigen; leukocyte antigen class II; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen HLA-DR-beta; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; p33; XXbac-BPG161M6.1

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Gene Aliases: CD74; DHLAG; DR-4; DR4; DRB1; DRB4; DRw10; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; Ia-GAMMA; II; MLRW; SS1

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UniProt ID: (Human) P04233, (Human) P01903, (Human) Q5UBA2, (Human) P79483, (Human) P13762, (Human) Q30154

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Entrez Gene ID: (Human) 972, (Human) 3122, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127

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Function(s)

beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding MHC class II receptor activity peptide antigen binding major histocompatibility complex protein scaffold/adaptor protein

Process(es)

activation of MAPK activity prostaglandin biosynthetic process positive regulation of cytokine-mediated signaling pathway positive regulation of dendritic cell antigen processing and presentation negative regulation of peptide secretion positive regulation of type 2 immune response negative regulation of mature B cell apoptotic process protein complex assembly intracellular protein transport defense response signal transduction cell proliferation immunoglobulin mediated immune response antigen processing and presentation of endogenous antigen antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of B cell proliferation macrophage migration inhibitory factor signaling pathway regulation of macrophage activation negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator T cell selection positive thymic T cell selection negative thymic T cell selection negative regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration chaperone mediated protein folding requiring cofactor positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production peptide antigen assembly with MHC class II protein complex antigen processing and presentation of peptide or polysaccharide antigen via MHC class II polysaccharide assembly with MHC class II protein complex immune response viral process T cell costimulation T cell receptor signaling pathway cognition interferon-gamma-mediated signaling pathway

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HLA-DR Monoclonal Antibody (LN3), PE-Cyanine7, eBioscience™

HLA-DR Antibody (25-9956-42) in Flow

Product Details

25-9956-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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