Invitrogen

HK1 Monoclonal Antibody (4B7)

HK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

HK1 Antibody (MA5-49200) in ICC/IF

Immunocytochemistry analysis of HK1 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in HK1 Monoclonal antibody (Product # MA5-49200) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the... View More

HK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HK1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

Product Details

MA5-49200

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

4B7

Immunogen

E.coli-derived human Hexokinase 1/HK1 recombinant protein (Position: D17-R323).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074858

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

This gene encodes a member of the ARHGAP family of Rho/Rac/Cdc42-like GTPase activating proteins. The encoded protein interacts with the Ras-related protein Gem through its N-terminal domain. Separately, it interacts with RhoA through a RhoGAP domain, and stimulates RhoA-dependent GTPase activity. Alternatively spliced variants that encode different protein isoforms have been described but their full-length nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Brain form hexokinase; downeast anemia; glycolytic enzyme; hexokinase I; hexokinase IR; Hexokinase type I; Hexokinase, tumor isozyme; Hexokinase-1; Hexokinase-A; HK I

View more View less

Gene Aliases: BB404130; dea; HEXOKIN; hexokinase; HK; Hk-1; HK1; Hk1-s; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; mHk1-s

View more View less

UniProt ID: (Human) P19367, (Rat) P05708, (Mouse) P17710

View more View less

Entrez Gene ID: (Human) 3098, (Rat) 25058, (Mouse) 15275

View more View less

Function(s)

glucokinase activity hexokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity protein kinase activity ATPase activity protein complex binding protein homodimerization activity nucleotide binding catalytic activity kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor kinase

Process(es)

cellular glucose homeostasis glycolytic process glucose transport carbohydrate phosphorylation glucose 6-phosphate metabolic process canonical glycolysis establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion response to ischemia protein phosphorylation regulation of anion channel activity peptidyl-serine phosphorylation peptidyl-threonine phosphorylation peptidyl-tyrosine phosphorylation hexose metabolic process negative regulation of apoptotic process protein autophosphorylation carbohydrate metabolic process metabolic process phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HK1 Monoclonal Antibody (4B7)

HK1 Antibody (MA5-49200) in ICC/IF

Product Details

MA5-49200

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!