Invitrogen

HK1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (25) HK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

HK1 Antibody (PA5-87969) in ICC/IF

Immunofluorescence analysis of HK1 in NIH/3T3 cells. Samples were incubated with HK1 Polyclonal antibody (Product # PA5-87969) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

HK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HK1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

HK1 Antibody — Antibody specificity was demonstrated by knockout of the target protein. Loss of signal was observed for target protein in HK1 knockout (KO) 293T cell line using Anti-HK1 Polyclonal Antibody (Product # PA5-87969). {KO}

Product Details

PA5-87969

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-219 of human HK1 (NP_0001792)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.51 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804550

Product Specific Information

Immunogen sequence: KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGY

Positive Samples: MCF7, 293T, Jurkat, THP-1, NIH/3T3, K-562, Mouse skeletal muscle, rat testis; Cellular Location: Mitochondrion outer membrane

Target Information

This gene encodes a member of the ARHGAP family of Rho/Rac/Cdc42-like GTPase activating proteins. The encoded protein interacts with the Ras-related protein Gem through its N-terminal domain. Separately, it interacts with RhoA through a RhoGAP domain, and stimulates RhoA-dependent GTPase activity. Alternatively spliced variants that encode different protein isoforms have been described but their full-length nature has not been determined.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Brain form hexokinase; downeast anemia; glycolytic enzyme; hexokinase I; hexokinase IR; Hexokinase type I; Hexokinase, tumor isozyme; Hexokinase-1; Hexokinase-A; HK I

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Gene Aliases: BB404130; dea; HEXOKIN; hexokinase; HK; Hk-1; HK1; Hk1-s; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; mHk1-s

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UniProt ID: (Human) P19367, (Rat) P05708, (Mouse) P17710

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Entrez Gene ID: (Human) 3098, (Rat) 25058, (Mouse) 15275

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Function(s)

glucokinase activity hexokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity protein kinase activity ATPase activity protein complex binding protein homodimerization activity nucleotide binding catalytic activity kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor kinase

Process(es)

cellular glucose homeostasis glycolytic process glucose transport carbohydrate phosphorylation glucose 6-phosphate metabolic process canonical glycolysis establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion response to ischemia protein phosphorylation regulation of anion channel activity peptidyl-serine phosphorylation peptidyl-threonine phosphorylation peptidyl-tyrosine phosphorylation hexose metabolic process negative regulation of apoptotic process protein autophosphorylation carbohydrate metabolic process metabolic process phosphorylation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HK1 Polyclonal Antibody

HK1 Antibody (PA5-87969) in ICC/IF

Product Details

PA5-87969

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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