Invitrogen

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP

Rabbit IgG (H+L) Secondary Antibody in Western Blot (WB)

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Rabbit IgG (H+L) Secondary Antibody (32460) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of K562 (Lane 1) and PC-3 (Lane 2). The blots were probed with Anti-Cyclophilin B Rabbit Polyclonal Antibody (Product # TAB1002, 0.5 µg/mL) and detected using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Product # 32460) at dilutions 1:500 (Fig. 1), 1:2,000 (Fig. 2) and 1:5,000 (Fig. 3). A 23 kDa band corresponding to Cy... View More

Rabbit IgG (H+L) Secondary Antibody in Immunohistochemistry (IHC)

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Product Details

32460

Applications

Tested Dilution

Western Blot (WB)

1:500-1:5,000

Immunohistochemistry (IHC)

1:6-1:60

ELISA (ELISA)

1:60-1:500

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Purified Rabbit IgG, whole molecule

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

0.01 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_1185567

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

This antibody may cross-react with immunoglobulins from other species.

Working dilutions for Western blotting using SuperSignal West Substrates:
SuperSignal West Pico Substrate - 1:250-1:1250
SuperSignal West Dura Substrate - 1:600-1:3000
SuperSignal West Femto Substrate - 1:1250-1:6000
ECL Western Blotting Substrate - 1:20-1:125

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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